For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    By product type
    Proteins and Peptides
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/mtco1-antibody-1d6e1a8-ab14705.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Subcellular Markers Organelles Mitochondria
Share by email
Recombinant

Recombinant Anti-MTCO1 antibody [1D6E1A8] (ab14705)

  • Datasheet
  • SDS
Reviews (42)Q&A (27)References (474)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1D6E1A8] to MTCO1
  • Suitable for: ICC, IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647

You may also be interested in

Primary
Product image
Anti-NDUFA9 antibody [20C11B11B11] (ab14713)
Primary
Product image
Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
Primary
Product image
Anti-MTCO1 antibody [EPR19628] (ab203912)

View more associated products

Overview

  • Product name

    Anti-MTCO1 antibody [1D6E1A8]
    See all MTCO1 primary antibodies
  • Description

    Mouse monoclonal [1D6E1A8] to MTCO1
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Goat, Cat, Dog, Pig, Caenorhabditis elegans, Zebrafish, Quail, Rhesus monkey, Chinese hamster, Common marmoset
  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF7, C6, PC-12, Neuro-2a, RAW 264.7 whole cell lysates. Human skeletal, Rat heart and Rat liver tissue lysates. IHC-P: Human kidney, Mouse colon, and Rat colon tissue. ICC: Human cervical adenocarcinoma, Mouse neuroblastoma neuroblast, and rat glial tumor cells. Flow Cyt: HeLa, Neuro-2a, and C6 cells.
  • General notes

    This product has switched from a hybridoma to recombinant production method on 24th May 2023.


    Western blot protocol advice:

    For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal.

    Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

    This antibody clone [1D6E1A8] is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    1D6E1A8
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex IV
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)
    • Alexa Fluor® 647 Anti-MTCO1 antibody [1D6E1A8] (ab198600)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Positive Controls

    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab14705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC (2)
1/100.
IHC-P (13)
1/5000.
WB (18)
1/1000.
Flow Cyt (1)
1/500.
Notes
ICC
1/100.
IHC-P
1/5000.
WB
1/1000.
Flow Cyt
1/500.

Target

  • Function

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
  • Pathway

    Energy metabolism; oxidative phosphorylation.
  • Involvement in disease

    Defects in MT-CO1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-CO1 are a cause of anemia sideroblastic acquired idiopathic (AISA) [MIM:516030]; a disease characterized by inadequate formation of heme and excessive accumulation of iron in mitochondria.
    Defects in MT-CO1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in MT-CO1 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.
    Defects in MT-CO1 are a cause of deafness sensorineural mitochondrial (DFNM) [MIM:500008]. DFNM is a form of non-syndromic deafness with maternal inheritance. Affected individuals manifest progressive, postlingual, sensorineural hearing loss involving high frequencies.
    Defects in MT-CO1 are a cause of colorectal cancer (CRC) [MIM:114500].
  • Sequence similarities

    Belongs to the heme-copper respiratory oxidase family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P00395 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2565700 Caenorhabditis elegans
    • Entrez Gene: 4512 Human
    • Entrez Gene: 17708 Mouse
    • Entrez Gene: 26195 Rat
    • Entrez Gene: 140539 Zebrafish
    • Omim: 516030 Human
    • SwissProt: P24893 Caenorhabditis elegans
    • SwissProt: Q9ZZ64 Dog
    • SwissProt: P00395 Human
    • SwissProt: P00397 Mouse
    • SwissProt: O79876 Pig
    • SwissProt: P05503 Rat
    • SwissProt: O78749 Sheep
    • SwissProt: Q9MIY8 Zebrafish
    see all
  • Alternative names

    • COI antibody
    • COX I antibody
    • COX1 antibody
    • COX1_HUMAN antibody
    • COXI antibody
    • Cytochrome c oxidase polypeptide I antibody
    • Cytochrome c oxidase subunit 1 antibody
    • Cytochrome C Oxidase subunit I antibody
    • Mitochondrially encoded cytochrome c oxidase I antibody
    • MT CO1 antibody
    • MT-CO1 antibody
    • MTCO 1 antibody
    • MTCO1 antibody
    see all

Images

  • Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    All lanes : Anti-MTCO1 antibody [1D6E1A8] (ab14705) at 1/1000 dilution

    Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) non-mitochondrial fraction
    Lane 2 : HeLa mitochondrial fraction
    Lane 3 : MCF7(human breast adenocarcinoma epithelial cell) non-mitochondrial fraction
    Lane 4 : MCF7 mitochondrial fraction

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 35 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    All lanes : Anti-MTCO1 antibody [1D6E1A8] (ab14705) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
    Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 4 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 5 : Human skeletal muscle tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 35 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human kidney. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse colon. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Rat colon. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human cervical adenocarcinoma epithelial cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Human cervical adenocarcinoma epithelial cells. ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse neuroblastoma neuroblast cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Mouse neuroblastoma neuroblast cells. ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat glial tumor cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Rat glial tumor cells. ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Flow cytometric analysis of Human HeLa cells labelling MTCO1 with ab14705 at 1/500 dilution (Right) compared with an isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at was used as the secondary antibody. Gated on viable cells.

  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Flow cytometric analysis of Neuro-2a cells labelling MTCO1 with ab14705 at 1/500 dilution (Right) compared with an isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at was used as the secondary antibody. Gated on viable cells.

  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    Flow cytometric analysis of C6 cells labelling MTCO1 with ab14705 at 1/500 dilution (Right) compared with an isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at was used as the secondary antibody. Gated on viable cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)Image from Phillips J et al., Sci Rep. 2016 May 16;6:26013. Fig 3b doi: 10.1038/srep26013. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    This image was generated using a previous batch manufactured using hybridoma production method.

    250 μm human cerebellar sections from control individuals and a patient with mitochondrial disease underwent passive clearing at 37 °C for 2 or 4 weeks.

    The quality of immunofluorescent staining is determined by duration of passive clearing; 2 weeks of passive clearing produced minimal labelling of the white matter in the granule cell layer (NF-H; green; 488 nm and MBP; red, 546 nm) with an absence of labelling of mitochondria (MTCO1 (COXI) (ab14705, 1/100); purple; 647 nm; Extending passive clearing to 4 weeks improved the quality of stain with identifiable Purkinje cells and their axons (NF-H, green; 488 nm) and their myelin sheaths (MBP; red, 546 nm) and mitochondria (MTCO1 (COXI) (ab14705, 1/100); purple; 647 nm.

Protocols

  • Mouse on Mouse staining protocol

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (474)

Publishing research using ab14705? Please let us know so that we can cite the reference in this datasheet.

ab14705 has been referenced in 474 publications.

  • Aoki H  et al. Thymidine Kinase 2 and Mitochondrial Protein COX I in the Cerebellum of Patients with Spinocerebellar Ataxia Type 31 Caused by Penta-nucleotide Repeats (TTCCA)n. Cerebellum 22:70-84 (2023). PubMed: 35084690
  • Xu Z  et al. Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength. Nat Commun 13:894 (2022). PubMed: 35173176
  • Bury AG  et al. A subcellular cookie cutter for spatial genomics in human tissue. Anal Bioanal Chem 414:5483-5492 (2022). PubMed: 35233697
  • Zhao Y  et al. ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models. Nat Commun 13:1121 (2022). PubMed: 35236834
  • Kiyokoba R  et al. Mitochondrial dysfunction-induced high hCG associated with development of fetal growth restriction and pre-eclampsia with fetal growth restriction. Sci Rep 12:4056 (2022). PubMed: 35260712
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 18 Abreviews or Q&A

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
60 µg
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 02 2021

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
15 µg
Specification
SH-SY5Y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 23 2018

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - other (Mitochondrial extract)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
25 µg
Specification
Mitochondrial extract
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 08 2018

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Drosophila melanogaster Tissue lysate - nuclear (brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (NuPage Gels 12% BT gels)
Loading amount
20 µg
Specification
brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 21 2017

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Drosophila melanogaster Cell lysate - other (whole fly mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
50 µg
Specification
whole fly mitochondrial fraction
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 12 2017

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - other (Fibroblasts, mitochondrial fraction.)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
25 µg
Specification
Fibroblasts, mitochondrial fraction.
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 06 2017

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
American Robin (Turdis migratorius) Cell lysate - other (Fibroblasts, mitochondrial fraction.)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
25 µg
Specification
Fibroblasts, mitochondrial fraction.
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 06 2017

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Retina, RPE)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina, RPE
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 25 2017

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Mouse retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 18 2016

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE19, hfRPE)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
ARPE19, hfRPE
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 18 2016

1-10 of 18 Abreviews or Q&A

  •  Previous
  • 1
  • 2
  • Next 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.