Anti-MTCO2 antibody [12C4F12] (ab110258)
![Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258)](https://www.abcam.com/ps/products/110/ab110258/Images/ab110258-123443-anti-mtco2-antibody-12c4f12-western-blot.jpg "Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258)")
Key features and details
- Mouse monoclonal [12C4F12] to MTCO2
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-MTCO2 antibody [12C4F12]
See all MTCO2 primary antibodies -
Description
Mouse monoclonal [12C4F12] to MTCO2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- WB: Mitochondrial lysate from human heart tissue. ICC/IF: Cultured human embryonic lung derived fibroblasts. Flow Cyt: HeLa cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab110258 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110258 was judged near homogeneity by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
12C4F12 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (11) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
|
| ICC/IF | (2) |
Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal)
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. |
|
ICC/IF
Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal) |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. -
Involvement in disease
Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. -
Sequence similarities
Belongs to the cytochrome c oxidase subunit 2 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4513 Human
- Omim: 516040 Human
- SwissProt: P00403 Human
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Alternative names
- COII antibody
- COX 2 antibody
- COX II antibody
see all
Images
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Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258)Anti-MTCO2 antibody [12C4F12] (ab110258) at 1 µg/ml + Mitochondrial lysate from human heart tissue at 5 µg
Predicted band size: 26 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (ab110258)](https://www.abcam.com/ps/products/110/ab110258/Images/ab110258-123442-anti-mtco2-antibody-12c4f12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (ab110258)Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 µg/ml. An Alexa Fluor® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.
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![Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (ab110258)](https://www.abcam.com/ps/products/110/ab110258/Images/ab110258-123441-anti-mtco2-antibody-12c4f12-flow-cytometry.jpg)
Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (ab110258)ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue).An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (198)
ab110258 has been referenced in 198 publications.
- Meng F et al. A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation. Nucleic Acids Res 49:1075-1093 (2021). PubMed: 33398350
- Sun X et al. Mitochondrial gene COX2 methylation and downregulation is a biomarker of aging in heart mesenchymal stem cells. Int J Mol Med 47:161-170 (2021). PubMed: 33416107
- Inokuchi S et al. Suppression of optineurin impairs the progression of hepatocellular carcinoma through regulating mitophagy. Cancer Med 10:1501-1514 (2021). PubMed: 33600074
- Inak G et al. Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome. Nat Commun 12:1929 (2021). PubMed: 33771987
- D'Angelo L et al. NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate. Cell Rep 35:109002 (2021). PubMed: 33882309