For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/mtco2-antibody-4b12a5-ab110271.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Mitochondrial
Share by email

Anti-MTCO2 antibody [4B12A5] (ab110271)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (36)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-MTCO2 antibody [4B12A5] (ab110271)

    Key features and details

    • Mouse monoclonal [4B12A5] to MTCO2
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG2a

    You may also be interested in

    Primary
    Product image
    Anti-VDAC1/Porin antibody [16G9E6BC4] - Mitochondrial Loading Control (ab110326)
    Primary
    Product image
    Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)
    Primary
    Product image
    Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195883)

    View more associated products

    Overview

    • Product name

      Anti-MTCO2 antibody [4B12A5]
      See all MTCO2 primary antibodies
    • Description

      Mouse monoclonal [4B12A5] to MTCO2
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Saccharomyces cerevisiae
    • Immunogen

      Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • Mitochondria from yeast membrane extract
    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

      Product was previously marketed under the MitoSciences sub-brand.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze.
    • Storage buffer

      pH: 7.5
      Preservative: 0.02% Sodium azide
      Constituent: HEPES buffered saline
    • Concentration information loading...
    • Purity

      IgG fraction
    • Purification notes

      Near homogeneity as judged by SDS-PAGE. ab110271 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
    • Clonality

      Monoclonal
    • Clone number

      4B12A5
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Integration of energy metabolism
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Integration of energy
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Oxidative phosphorylation
      • Complex IV
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Cytochromes
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab110271 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 2 µg/ml. Predicted molecular weight: 26 kDa.
    Notes
    WB
    Use a concentration of 2 µg/ml. Predicted molecular weight: 26 kDa.

    Target

    • Function

      Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
    • Involvement in disease

      Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    • Sequence similarities

      Belongs to the cytochrome c oxidase subunit 2 family.
    • Cellular localization

      Mitochondrion inner membrane.
    • Target information above from: UniProt accession P00403 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 285521 Saccharomyces cerevisiae
      • SwissProt: P00410 Saccharomyces cerevisiae
      • Alternative names

        • COII antibody
        • COX 2 antibody
        • COX II antibody
        • COX2 antibody
        • COX2_HUMAN antibody
        • COXII antibody
        • Cytochrome c oxidase II antibody
        • Cytochrome c oxidase polypeptide II antibody
        • Cytochrome c oxidase subunit 2 antibody
        • MT CO2 antibody
        • MT-CO2 antibody
        • MTCO2 antibody
        see all

      Images

      • Western blot - Anti-MTCO2 antibody [4B12A5] (ab110271)
        Western blot - Anti-MTCO2 antibody [4B12A5] (ab110271)
        Anti-MTCO2 antibody [4B12A5] (ab110271) at 2 µg/ml + Mitochondria from yeast membrane extract at 10 µg

        Predicted band size: 26 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (36)

      Publishing research using ab110271? Please let us know so that we can cite the reference in this datasheet.

      ab110271 has been referenced in 36 publications.

      • Lee J  et al. The plasticity of the pyruvate dehydrogenase complex confers a labile structure that is associated with its catalytic activity. PLoS One 15:e0243489 (2020). PubMed: 33370314
      • Hijazi I  et al. Retrograde signaling mediates an adaptive survival response to endoplasmic reticulum stress. J Cell Sci N/A:N/A (2020). WB . PubMed: 32005698
      • Hose J  et al. The genetic basis of aneuploidy tolerance in wild yeast. Elife 9:N/A (2020). PubMed: 31909711
      • Ghosh S  et al. An essential role for cardiolipin in the stability and function of the mitochondrial calcium uniporter. Proc Natl Acad Sci U S A 117:16383-16390 (2020). PubMed: 32601238
      • Bohovych I  et al. Redox Regulation of the Mitochondrial Quality Control Protease Oma1. Antioxid Redox Signal 31:429-443 (2019). PubMed: 31044600
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-MTCO2 antibody [4B12A5]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Candida albicans Cell lysate - other (Cytosol and mitochondrial fraction)
      Gel Running Conditions
      Reduced Denaturing (4-12% precast gel)
      Loading amount
      15 µg
      Treatment
      Induced with 10 mM proline for 1 h
      Specification
      Cytosol and mitochondrial fraction
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Fitz Gerald S. Silao

      Verified customer

      Submitted Apr 26 2021

      Question

      I would like to know if the epitopes recognized by the following antibodies are known:
      Code
      Name
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-12C4F12--ab110258.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-12C4F12--ab110258.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-12C4F12--ab110258.html
      Mouse monoclonal
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3314-ab79393.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3314-ab79393.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3314-ab79393.html
      Rabbit monoclonal
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-4B12A5-ab110271.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-4B12A5-ab110271.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-4B12A5-ab110271.html
      Mouse monoclonal
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab91317.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab91317.html
      Rabbit polyclonal
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3313-ab109739.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3313-ab109739.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-EPR3313-ab109739.html
      Rabbit monoclonal
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab101802.html
      https://www.abcam.com/Cytochrome-C-oxidase-subunit-II-antibody-ab101802.html
      Rabbit polyclonal
      Awaiting your kind reply, I remain

      Read More

      Abcam community

      Verified customer

      Asked on Sep 21 2012

      Answer

      Thank you for contacting us.

      The immunogens are as follows;

      ab79393; A synthetic peptide corresponding to residues in human Cytochrome C oxidase subunit II
      ab109739; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
      ab101802; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
      ab91317; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
      ab110258: exact epitope is not determined. Antigen was full length protein
      ab110271: exact epitope is not determined. Antigen was full length protein

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Sep 21 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2023 Abcam plc. All rights reserved.