Anti-MTCO2 antibody [4B12A5] (ab110271)
![Western blot - Anti-MTCO2 antibody [4B12A5] (ab110271)](https://www.abcam.com/ps/products/110/ab110271/Images/ab110271-123464-anti-mtco2-antibody-4b12a5-western-blot.jpg "Western blot - Anti-MTCO2 antibody [4B12A5] (ab110271)")
Key features and details
- Mouse monoclonal [4B12A5] to MTCO2
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG2a
Overview
-
Product name
Anti-MTCO2 antibody [4B12A5]
See all MTCO2 primary antibodies -
Description
Mouse monoclonal [4B12A5] to MTCO2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Mitochondria from yeast membrane extract
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110271 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
4B12A5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 26 kDa.
|
| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 26 kDa. |
Target
-
Function
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. -
Involvement in disease
Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. -
Sequence similarities
Belongs to the cytochrome c oxidase subunit 2 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
-
Database links
- Entrez Gene: 285521 Saccharomyces cerevisiae
- SwissProt: P00410 Saccharomyces cerevisiae
-
Alternative names
- COII antibody
- COX 2 antibody
- COX II antibody
see all
Images
-
Western blot - Anti-MTCO2 antibody [4B12A5] (ab110271)Anti-MTCO2 antibody [4B12A5] (ab110271) at 2 µg/ml + Mitochondria from yeast membrane extract at 10 µg
Predicted band size: 26 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (37)
ab110271 has been referenced in 37 publications.
- Dietz JV et al. Mitochondrial contact site and cristae organizing system (MICOS) machinery supports heme biosynthesis by enabling optimal performance of ferrochelatase. Redox Biol 46:102125 (2021). PubMed: 34517185
- Hijazi I et al. Retrograde signaling mediates an adaptive survival response to endoplasmic reticulum stress. J Cell Sci N/A:N/A (2020). WB . PubMed: 32005698
- Hose J et al. The genetic basis of aneuploidy tolerance in wild yeast. Elife 9:N/A (2020). PubMed: 31909711
- Ghosh S et al. An essential role for cardiolipin in the stability and function of the mitochondrial calcium uniporter. Proc Natl Acad Sci U S A 117:16383-16390 (2020). PubMed: 32601238
- Lee J et al. The plasticity of the pyruvate dehydrogenase complex confers a labile structure that is associated with its catalytic activity. PLoS One 15:e0243489 (2020). PubMed: 33370314