Name: Anti-MTCO2 antibody [EPR3314]
Brand: RabMAb
SKU: ab79393
Rating: 5 (2 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-MTCO2 antibody [EPR3314] (ab79393)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3314] to MTCO2
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
Reacts with: Human

Alexa Fluor® 647 Carrier Free HRP

Product name

Anti-MTCO2 antibody [EPR3314]
See all MTCO2 primary antibodies
Description

Rabbit monoclonal [EPR3314] to MTCO2
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human MTCO2 aa 200-300. The exact sequence is proprietary.
Positive control
- K562, MCF7, THP1 and HeLa cell lysates; human heart, liver and heart muscle tissues. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3314
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 membrane extract lysate (ab29539)
- HeLa membrane extract lysate (ab29547)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab79393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF		Use a concentration of 5 µg/ml.
WB	(2)	1/5000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 25 kDa).
IP		1/50.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
Flow Cyt (Intra) 1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/5000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 25 kDa).
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Involvement in disease

Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
Sequence similarities

Belongs to the cytochrome c oxidase subunit 2 family.
Cellular localization

Mitochondrion inner membrane.
Target information above from: UniProt accession P00403 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4513 Human
- Omim: 516040 Human
- SwissProt: P00403 Human
Alternative names
- COII antibody
- COX 2 antibody
- COX II antibody
- COX2 antibody
- COX2_HUMAN antibody
- COXII antibody
- Cytochrome c oxidase II antibody
- Cytochrome c oxidase polypeptide II antibody
- Cytochrome c oxidase subunit 2 antibody
- MT CO2 antibody
- MT-CO2 antibody
- MTCO2 antibody
see all

Western blot - Anti-MTCO2 antibody [EPR3314] (ab79393)

All lanes : Anti-MTCO2 antibody [EPR3314] (ab79393) at 1/10000 dilution

Lane 1 : K562 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : THP1 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 25 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [EPR3314] (ab79393)

ab79393 at 1/100 dilution staining Cytochrome C oxidase subunit II in Human Liver (A), Human Heart(B) and HeLa Cell Line(C) by Immunohistochemistry, Paraffin-embedded tissues. Detection method of A and B was HRP-conjugated anti-rabbit with DAB substrate used for staining.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [EPR3314] (ab79393)

ICC/IF image of ab79393 stained HepG2 cells. The cells were 100% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79393, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunoprecipitation - Anti-MTCO2 antibody [EPR3314] (ab79393)

Purified ab79393 at 1/50 dilution (2µg) immunoprecipitating MTCO2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab79393 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab79393 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 21 kDa
Flow Cytometry (Intracellular) - Anti-MTCO2 antibody [EPR3314] (ab79393)

Overlay histogram showing HepG2 cells stained with ab79393 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79393, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-MTCO2 antibody [EPR3314] (ab79393)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab79393? Please let us know so that we can cite the reference in this datasheet.

ab79393 has been referenced in 30 publications.

Remsik J et al. Leptomeningeal metastatic cells adopt two phenotypic states. Cancer Rep (Hoboken) 5:e1236 (2022). PubMed: 33372403
Bertola N et al. Mutated FANCA Gene Role in the Modulation of Energy Metabolism and Mitochondrial Dynamics in Head and Neck Squamous Cell Carcinoma. Cells 11:N/A (2022). PubMed: 35954197
Fang Q et al. Targeting mitochondrial tyrosyl-tRNA synthetase YARS2 suppresses colorectal cancer progression. Cancer Biol Ther 23:1-8 (2022). PubMed: 36154909
Yang W et al. Downregulation of lncRNA ZNF582-AS1 due to DNA hypermethylation promotes clear cell renal cell carcinoma growth and metastasis by regulating the N(6)-methyladenosine modification of MT-RNR1. J Exp Clin Cancer Res 40:92 (2021). PubMed: 33691743
Aminzadeh-Gohari S et al. Targeting Mitochondria in Melanoma. Biomolecules 10:N/A (2020). PubMed: 33007949

View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa WT vs HeLa rho-0)

Loading amount

13 µg

Specification

HeLa WT vs HeLa rho-0

Gel Running Conditions

Reduced Denaturing (Reduced Denaturing (4-15%))

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 31 2012

Application

Western blot

Sample

Human Cell lysate - whole cell (PBMC)

Loading amount

20 µg

Specification

PBMC

Gel Running Conditions

Reduced Denaturing

Blocking step

Fluorescence special buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 04 2011

Question

Abcam community

Verified customer

Asked on Sep 21 2012

Answer

Thank you for contacting us.

The immunogens are as follows;

ab79393; A synthetic peptide corresponding to residues in human Cytochrome C oxidase subunit II
ab109739; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
ab101802; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
ab91317; A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.
ab110258: exact epitope is not determined. Antigen was full length protein
ab110271: exact epitope is not determined. Antigen was full length protein

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Abcam Scientific Support

Answered on Sep 21 2012

Question

I'm pretty new to using antibodies, so I have a really basic question. When diluting an antibody for IHC or ICC, what do you dilute it with. Specifically we are using the anti-COX II ab79393 and anti-amyloid beta ab11132. What buffers or solutions do you recommend for diluting these antibodies?

Abcam community

Verified customer

Asked on Jun 07 2012

Answer

Thank you for contacting Abcam.

For IHC and ICC, antibodies are typically diluted in PBS or in a 1-3% BSA in PBS solution. Some researchers will use TBS instead, but either is fine. I have included links below to our IHC and ICC protocols for your reference.

https://www.abcam.com/index.html?pageconfig=resource&rid=11384

https://www.abcam.com/index.html?pageconfig=resource&rid=11417

I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Abcam Scientific Support

Answered on Jun 07 2012

Question

Inquiry: I am using product ab79393, anti-Cytochrome C oxidase subunit II antibody for IHC in paraffin for research purposes. I now need to run an IgG negative control and have located your product ab25938, rabbit IgG monoclonal (SP137) isotype control, and now need to know the protein concentrations of EACH of the products in order to do so. Thank you in advance for your prompt response!

Abcam community

Verified customer

Asked on May 23 2012

Answer

Thank you for your enquiry.

I have confirmed that ab79393, anti-Cytochrome C oxidase subunit II antibody antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml.

While we do not offer a product with catalog number ab25938 our Rabbit IgG, monoclonal [SP137] - isotype control with designation ab128142. We recommend using this product at a dilution of 1:100 for IHC-P control.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Abcam Scientific Support

Answered on May 23 2012

Question

What is the immunogen seq for this ab? I would like to use it on cat an rat tissue.

Abcam community

Verified customer

Asked on Nov 15 2011

Answer

The immunogen of ab79393 shares 70% identity with rat Cytochrome c oxidase subunit 2. It also shares 70% identity with cat. Based on these number I would not predict cross-reactivity with either species.I have looked through the other antibodies in our catalogue and I do not think that they will react either cat or rat tissue.

Abcam Scientific Support

Answered on Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (30)

Customer reviews and Q&As

Western blot abreview for Anti-MTCO2 antibody [EPR3314]

Western blot abreview for Anti-MTCO2 antibody [EPR3314]