Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)
![Western blot - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)](https://www.abcam.com/ps/products/156/ab156031/Images/ab156031-163386-anti-mtco2-antibody-mm7d-5a11ca9-western-blot.jpg "Western blot - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)")
Key features and details
- Mouse monoclonal [MM7D-5A11CA9] to MTCO2
- Suitable for: ICC/IF, WB, Flow Cyt, IP
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-MTCO2 antibody [MM7D-5A11CA9]
See all MTCO2 primary antibodies -
Description
Mouse monoclonal [MM7D-5A11CA9] to MTCO2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human, African green monkey
Does not react with: Mouse, Rat -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Human liver and heart mitochondrial extracts; Human heart homogenate; 143B(Human osteosarcoma), HeLa and COS7 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab156031 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
MM7D-5A11CA9 -
Isotype
IgG1 -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
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| WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 25.5 kDa).
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
|
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 25.5 kDa). |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IP
Use at an assay dependent concentration. |
Target
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Function
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. -
Involvement in disease
Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. -
Sequence similarities
Belongs to the cytochrome c oxidase subunit 2 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4513 Human
- Omim: 516040 Human
- SwissProt: P00403 Human
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Alternative names
- COII antibody
- COX 2 antibody
- COX II antibody
see all
Images
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Western blot - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)All lanes : Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031) at 5 µg/ml
Lane 1 : Human Liver Mitochondria Extract
Lane 2 : Human Heart Mitochondria Extrac
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution
Predicted band size: 25.5 kDa
1X Blocking Buffer (ab126587) in H2O was used for blocking and incubation steps. -
![Immunoprecipitation - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)](https://www.abcam.com/ps/products/156/ab156031/Images/ab156031-163385-anti-mtco2-antibody-mm7d-5a11ca9-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)Immunoprecipitation Coomassie stained gel for Anti-COXII antibody (ab156031).
Lane 1: Ladder
Lane 2: COXII Immunoprecipitated from 1mg Human Heart Homogenate (HHH) Extract. ab156031 anti-COXII captures the entire Cytochrome c oxidase complex in immunoprecipitation. -
![Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)](https://www.abcam.com/ps/products/156/ab156031/Images/ab156031-163384-anti-mtco2-antibody-mm7d-5a11ca9-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)Immunocytochemical analysis of 143B (Human osteosarcoma) wild type (WT) and mtDNA lacking (?0) cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labeling COXII with ab156031 at 1µg/ml (red) and anti-HSP60 (ab46798) at 1/1000 (green) as a counter stain. Secondary: Alexa 594 goat anti-mouse IgG at 1/1000 and Alexa 488 goat anti-rat IgG at 1/1000. ab156031 shows good reactivity with the wild type (143B-WT) cell line, but shows no signal with the mtDNA lacking (143B-?0) cell line. Cytochrome c oxidase 2 is exclusively encoded by mitochondrial DNA. The anti-HSP60 counter stain antibody identified mitochondria in both cell lines. -
![Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)](https://www.abcam.com/ps/products/156/ab156031/Images/ab156031-163383-anti-mtco2-antibody-mm7d-5a11ca9-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)Immunocytochemical analysis of COS7 (monkey kidney fibroblast) cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labeling COXII with ab156031 at 1µg/ml (red). Secondary: Alexa 594 goat anti-mouse IgG at 1/1000. ab156031 shows good reactivity with the COS7 cell type indicating cross reactivity with monkey cytochrome c oxidase. -
![Flow Cytometry - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)](https://www.abcam.com/ps/products/156/ab156031/Images/ab156031-163382-anti-mtco2-antibody-mm7d-5a11ca9-flow-cytometry.jpg)
Flow Cytometry - Anti-MTCO2 antibody [MM7D-5A11CA9] (ab156031)Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labeling COXII with ab156031 (blue) at 1µg/ml and an equal amount of an isotype control antibody (red).
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab156031 has been referenced in 4 publications.
- Ou L et al. Sialylation of FGFR1 by ST6Gal-I overexpression contributes to ovarian cancer cell migration and chemoresistance. Mol Med Rep 21:1449-1460 (2020). PubMed: 32016470
- Wright JN et al. Acute increases in O-GlcNAc indirectly impair mitochondrial bioenergetics through dysregulation of LonP1-mediated mitochondrial protein complex turnover. Am J Physiol Cell Physiol 316:C862-C875 (2019). PubMed: 30865517
- Lee AR et al. Involvement of mitochondrial biogenesis during the differentiation of human periosteum-derived mesenchymal stem cells into adipocytes, chondrocytes and osteocytes. Arch Pharm Res 42:1052-1062 (2019). PubMed: 31802425
- Djeungoue-Petga MA et al. Intramitochondrial Src kinase links mitochondrial dysfunctions and aggressiveness of breast cancer cells. Cell Death Dis 10:940 (2019). PubMed: 31819039
