Anti-MTCO3 antibody [DA5BC4] (ab110259)
![Western blot - Anti-MTCO3 antibody [DA5BC4] (ab110259)](https://www.abcam.com/ps/products/110/ab110259/Images/ab110259-123444-anti-mtco3-antibody-da5bc4-western-blot.jpg "Western blot - Anti-MTCO3 antibody [DA5BC4] (ab110259)")
Key features and details
- Mouse monoclonal [DA5BC4] to MTCO3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae
- Isotype: IgG2a
Overview
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Product name
Anti-MTCO3 antibody [DA5BC4] -
Description
Mouse monoclonal [DA5BC4] to MTCO3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Isolated mitochondria from Human , Rat and Mouse hearts.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab110259 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Near homogeneity ab110259 was judged as near homogeneity by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
DA5BC4 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 30 kDa.
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| Notes |
|---|
|
WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 30 kDa. |
Target
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Function
Subunits I, II and III form the functional core of the enzyme complex. -
Involvement in disease
Defects in MT-CO3 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
Defects in MT-CO3 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
Defects in MT-CO3 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine. -
Sequence similarities
Belongs to the cytochrome c oxidase subunit 3 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4514 Human
- Entrez Gene: 17710 Mouse
- Entrez Gene: 26204 Rat
- Omim: 516050 Human
- SwissProt: P00414 Human
- SwissProt: P00416 Mouse
- SwissProt: P05505 Rat
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Alternative names
- COIII antibody
- COX3 antibody
- COX3_HUMAN antibody
see all
Images
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Western blot - Anti-MTCO3 antibody [DA5BC4] (ab110259)All lanes : Anti-MTCO3 antibody [DA5BC4] (ab110259) at 4 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria Rat heart at 10 µg
Lane 3 : Isolated mitochondria from Mouse heart at 10 µg
Predicted band size: 30 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (38)
ab110259 has been referenced in 38 publications.
- Esparza-Moltó PB et al. Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition. PLoS Biol 19:e3001252 (2021). PubMed: 33983919
- Jiang C et al. Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1. Front Cell Dev Biol 8:603688 (2020). PubMed: 33409279
- Lee RG et al. Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis. J Cell Sci 133:N/A (2020). PubMed: 32576663
- Timón-Gómez A et al. Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis. Cell Rep 31:107607 (2020). PubMed: 32375044
- Xie Y et al. Mefloquine Inhibits Esophageal Squamous Cell Carcinoma Tumor Growth by Inducing Mitochondrial Autophagy. Front Oncol 10:1217 (2020). PubMed: 32850358