Recombinant Anti-mTOR antibody [EPR390(N)] (ab134903)
![Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)](https://www.abcam.com/ps/products/134/ab134903/Images/ab134903-315389-anti-mtor-antibody-epr390n-western-blot.jpg "Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR390(N)] to mTOR
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-mTOR antibody [EPR390(N)]
See all mTOR primary antibodies -
Description
Rabbit monoclonal [EPR390(N)] to mTOR -
Host species
Rabbit -
Specificity
Expression levels of the target protein vary with sample type and some optimisation may be required.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain, heart, liver and kidney lysates, Mouse brain heart, liver and kidney lysates, Rat brain, heart and kidney lysates, HeLa, Jurkat, MCF7, K562, Raji, HepG2, and HEK-293T cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR390(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000. Predicted molecular weight: 289 kDa.
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| Notes |
|---|
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WB
1/10000. Predicted molecular weight: 289 kDa. |
Target
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Function
Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. -
Tissue specificity
Expressed in numerous tissues, with highest levels in testis. -
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 7 HEAT repeats.
Contains 1 PI3K/PI4K domain. -
Post-translational
modificationsAutophosphorylated; when part of mTORC1 or mTORC2. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB. - Information by UniProt
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Database links
- Entrez Gene: 2475 Human
- Entrez Gene: 56717 Mouse
- Entrez Gene: 56718 Rat
- Omim: 601231 Human
- SwissProt: P42345 Human
- SwissProt: Q9JLN9 Mouse
- SwissProt: P42346 Rat
- Unigene: 338207 Human
see all -
Alternative names
- dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
- FK506 binding protein 12 rapamycin associated protein 1 antibody
- FK506 binding protein 12 rapamycin associated protein 2 antibody
see all
Images
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Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)All lanes : Anti-mTOR antibody [EPR390(N)] (ab134903) at 1/10000 dilution
Lane 1 : Wild-type HEK293T whole cell lysate
Lane 2 : MTOR knockout HEK293T whole cell lysate
Lane 3 : K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 289 kDaLanes 1 - 3: Merged signal (red and green). Green - ab134903 observed at 289 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab134903 was shown to specifically react with mTOR in wild-type HEK293T cells as signal was lost in MTOR knockout cells. Wild-type and MTOR knockout samples were subjected to SDS-PAGE. Ab134903 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)](https://www.abcam.com/ps/products/134/ab134903/Images/ab134903-4-anti-mtor-antibody-epr390n-western-blot-human-lysate.jpg)
Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)All lanes : Anti-mTOR antibody [EPR390(N)] (ab134903) at 1/10000 dilution
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : MTOR knockout HEK-293 cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : MTOR knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 289 kDaLanes 1 - 4: Merged signal (red and green). Green - ab134903 observed at 289 kDa. Red - loading control, ab130007 observed at 125 kDa.
ab134903 was shown to react with mTOR in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255411 (knockout cell lysate ab263789) was used. Wild-type and mTOR knockout samples were subjected to SDS-PAGE. ab134903 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)](https://www.abcam.com/ps/products/134/ab134903/Images/ab134903-344311-antimtorantibody-EPR390N-westernblot-breast-human.jpg)
Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)All lanes : Anti-mTOR antibody [EPR390(N)] (ab134903) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : Human brain lysates
Lane 4 : Mouse brain lysates
Lane 5 : Rat brain lysates
Lane 6 : Human liver lysates
Lane 7 : Mouse liver lysates
Lane 8 : Human heart lysates
Lane 9 : Mouse heart lysates
Lane 10 : Rat heart lysates
Lane 11 : Human kidney lysates
Lane 12 : Mouse kidney lysates
Lane 13 : Rat kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 289 kDa
Observed band size: 290 kDa why is the actual band size different from the predicted?Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBSTExposure time:
Lane 1 to 5: 80 seconds
Lane 6 to 13: 180 secondsThis antibody detects non-specific bands. It doesn’t detect the target band in some mouse and rat tissues.
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![Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)](https://www.abcam.com/ps/products/134/ab134903/Images/ab134903-149023-anti-mtor-antibody-epr390n-western-blot.jpg)
Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)All lanes : Anti-mTOR antibody [EPR390(N)] (ab134903) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDa -
![Anti-mTOR antibody [EPR390(N)] (ab134903)](https://www.abcam.com/ps/products/134/ab134903/Images/ab134903-6-benefits-of-recombinant-antibodies.png)
Anti-mTOR antibody [EPR390(N)] (ab134903)
Datasheets and documents
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SDS download
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Datasheet download
References (43)
ab134903 has been referenced in 43 publications.
- Huang WK et al. ARHGAP25 Inhibits Pancreatic Adenocarcinoma Growth by Suppressing Glycolysis via AKT/mTOR Pathway. Int J Biol Sci 17:1808-1820 (2021). PubMed: 33994864
- Su C et al. Roux-en-Y Gastric Bypass Improves Hepatic Glucose Metabolism Involving Upregulation of Sirt1 in Type 2 Diabetes Mellitus. Diabetes Metab Syndr Obes 14:2269-2280 (2021). PubMed: 34045876
- Liao YX et al. CXCR4 blockade sensitizes osteosarcoma to doxorubicin by inducing autophagic cell death via PI3K-Akt-mTOR pathway inhibition. Int J Oncol 59:N/A (2021). PubMed: 34080667
- Wang B et al. ANLN promotes carcinogenesis in oral cancer by regulating the PI3K/mTOR signaling pathway. Head Face Med 17:18 (2021). PubMed: 34082790
- Guo H et al. miR-125b-5p inhibits cell proliferation by targeting ASCT2 and regulating the PI3K/AKT/mTOR pathway in an LPS-induced intestinal mucosa cell injury model. Exp Ther Med 22:838 (2021). PubMed: 34149884