Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (ab119684)
Key features and details
- Mouse monoclonal [18G102B2E11] to mtTFA - Mitochondrial Marker
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker
See all mtTFA primary antibodies -
Description
Mouse monoclonal [18G102B2E11] to mtTFA - Mitochondrial Marker -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Does not react with: Mouse, Rat -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: human liver FFPE tissue sections
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab119684 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
18G102B2E11 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use a concentration of 1 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. -
Sequence similarities
Contains 2 HMG box DNA-binding domains. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 7019 Human
- Omim: 600438 Human
- SwissProt: Q00059 Human
- Unigene: 642966 Human
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Alternative names
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
see all
Images
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All lanes : Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (ab119684) at 1 µg/ml
Lane 1 : recombinant mtTFA (ab103784) at 0.025 µg
Lane 2 : HeLa whole cell lysate at 30 µg
Secondary
All lanes : HRP-conjugated Goat polyclonal to mouse IgG at 1/5000 dilution
Predicted band size: 29 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (ab119684)Immunocytochemistry image of ab119684 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (18G102B2E11, 1µg/ml) for 2h at room temperature. The secondary antibody was (green) 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). mtTFA localizes to the mitochondria.
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HeLa cells were stained with 2 µg/mL mtTFA antibody (ab119684) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (ab119684)IHC image of mtTFA staining in human liver HCC formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119684, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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Datasheet download
References (21)
ab119684 has been referenced in 21 publications.
- Alexander JF et al. Nasal administration of mitochondria reverses chemotherapy-induced cognitive deficits. Theranostics 11:3109-3130 (2021). PubMed: 33537077
- Hao Z et al. N6-Deoxyadenosine Methylation in Mammalian Mitochondrial DNA. Mol Cell 78:382-395.e8 (2020). PubMed: 32183942
- Maekawa H et al. Mitochondrial Damage Causes Inflammation via cGAS-STING Signaling in Acute Kidney Injury. Cell Rep 29:1261-1273.e6 (2019). PubMed: 31665638
- Li H et al. Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations. Autophagy 15:113-130 (2019). PubMed: 30160596
- Li Q et al. Ochratoxin A causes mitochondrial dysfunction, apoptotic and autophagic cell death and also induces mitochondrial biogenesis in human gastric epithelium cells. Arch Toxicol 93:1141-1155 (2019). PubMed: 30903243