Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)

Reviews (1) Submit a question References (13)
Western blot - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
  • Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
  • Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
  • Western blot - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
  • Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
  • Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
  • Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12285] to mtTFA - Mitochondrial Marker
  • Suitable for: IHC-P, WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

Overview

  • Product name

    Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker
    See all mtTFA primary antibodies

  • Description

    Rabbit monoclonal [EPR12285] to mtTFA - Mitochondrial Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human mtTFA aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q00059

  • Positive control

    • HeLa, A431, MCF7 and 293T cell lysates; Human kidney and prostate tissues; MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified, use 1/50 - 1/100.
WB
1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Notes
IHC-P
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified, use 1/50 - 1/100.
WB
1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.

Target

  • Function

    Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.

  • Sequence similarities

    Contains 2 HMG box DNA-binding domains.

  • Cellular localization

    Mitochondrion.
  • Information by UniProt

  • Database links

    • Alternative names

      • anscription factor 6-like 1 antibody
      • Mitochondrial transcription factor 1 antibody
      • mitochondrial transcription factor A antibody
      • MtTF1 antibody
      • mtTFA antibody
      • TCF 6 antibody
      • TCF-6 antibody
      • TCF6 antibody
      • TCF6L1 antibody
      • TCF6L2 antibody
      • TCF6L3 antibody
      • TFAM antibody
      • TFAM_HUMAN antibody
      • Transcription factor 6 antibody
      • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
      • Transcription factor 6 like 2 antibody
      • Transcription factor 6-like 2 antibody
      • transcription factor 6-like 3 antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial precursor antibody
      see all

    Images

    • Western blot - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      Western blot - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      All lanes : Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558) at 1/10000 dilution (purified)

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HEK293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Predicted band size: 29 kDa
      Observed band size: 24 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      Immunohistochemical staining of paraffin embedded human thyroid carcinoma with purified ab176558 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)

      Immunofluorescence staining of MCF7 cells with purified ab176558 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab176558 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)

      ab176558 (purified) at 1/40 immunoprecipitating mtTFA in 10 μg HEK293 (Lanes 1 and 2, observed at 24 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      Western blot - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      All lanes : Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558) at 1/10000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 29 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling mtTFA using unpurified ab176558 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue, labeling mtTFA using unpurified ab176558 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)

      Immunofluorescence analysis of MCF7 cells, labeling mtTFA using unpurified ab176558 at a 1/100 dilution.

    • Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      Immunoprecipitation - Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)

      Western blot analysis on Immunoprecipitation pellet from either 1) 293T cell lysate, or 2) 1xPBS (negative control), showing mtTFA, using unpurified ab176558 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)
      Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (ab176558)

    References (13)

    Publishing research using ab176558? Please let us know so that we can cite the reference in this datasheet.

    ab176558 has been referenced in 13 publications.

    • Li Y  et al. Sorafenib induces mitochondrial dysfunction and exhibits synergistic effect with cysteine depletion by promoting HCC cells ferroptosis. Biochem Biophys Res Commun 534:877-884 (2021). PubMed: 33162029
    • Špacková J  et al. Biochemical Background in Mitochondria Affects 2HG Production by IDH2 and ADHFE1 in Breast Carcinoma. Cancers (Basel) 13:N/A (2021). PubMed: 33916579
    • Zhao N  et al. Treadmill Exercise Attenuates Aß-Induced Mitochondrial Dysfunction and Enhances Mitophagy Activity in APP/PS1 Transgenic Mice. Neurochem Res 45:1202-1214 (2020). PubMed: 32125561
    • Liu Y  et al. Effect of TFAM on ATP content in tachypacing primary cultured cardiomyocytes and atrial fibrillation patients. Mol Med Rep 22:5105-5112 (2020). PubMed: 33174030
    • Chen C  et al. Investigation of mitochondrial biogenesis defects in single substantia nigra neurons using post-mortem human tissues. Neurobiol Dis 134:104631 (2020). PubMed: 31689514
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Feb 29 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com