Recombinant Anti-mtTFA antibody [EPR23548-120] (ab252432)
![Western blot - Anti-mtTFA antibody [EPR23548-120] (ab252432)](https://www.abcam.com/ps/products/252/ab252432/Images/ab252432-393117-anti-mttfa-antibody-epr23548120-western-blot-mouse-cerebellum-tissue-lysate-20-ug.jpg "Western blot - Anti-mtTFA antibody [EPR23548-120] (ab252432)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23548-120] to mtTFA
- Suitable for: IP, ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-mtTFA antibody [EPR23548-120]
See all mtTFA primary antibodies -
Description
Rabbit monoclonal [EPR23548-120] to mtTFA -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WB, Flow Cyt (Intra)more details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C2C12, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse cerebellum, heart and kidney tissue lysates;, Rat cerebellum and heart tissue lysates. ICC/IF: C2C12 and PC-12 cells. Flow Cyt (intra): C2C12 cells. IP: C2C12 and PC-12 cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23548-120 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| WB |
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 28 kDa).
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| Notes |
|---|
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 28 kDa). |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. -
Sequence similarities
Contains 2 HMG box DNA-binding domains. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 21780 Mouse
- Entrez Gene: 83474 Rat
- SwissProt: P40630 Mouse
- SwissProt: Q91ZW1 Rat
- Unigene: 229292 Mouse
- Unigene: 18266 Rat
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Alternative names
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
see all
Images
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Western blot - Anti-mtTFA antibody [EPR23548-120] (ab252432)All lanes : Anti-mtTFA antibody [EPR23548-120] (ab252432) at 1/1000 dilution
Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Rat cerebellum tissue lysate
Lane 5 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Flow Cytometry (Intracellular) - Anti-mtTFA antibody [EPR23548-120] (ab252432)](https://www.abcam.com/ps/products/252/ab252432/Images/ab252432-3-anti-mttfa-antibody-epr23548120-flow-cytometry-c2c12.jpg)
Flow Cytometry (Intracellular) - Anti-mtTFA antibody [EPR23548-120] (ab252432)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labelling mtTFA with ab252432 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR23548-120] (ab252432)](https://www.abcam.com/ps/products/252/ab252432/Images/ab252432-1-anti-mttfa-antibody-epr23548120-immunocytochemistry-c2c12.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR23548-120] (ab252432)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 cells labelling mtTFA with ab252432 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.
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![Immunoprecipitation - Anti-mtTFA antibody [EPR23548-120] (ab252432)](https://www.abcam.com/ps/products/252/ab252432/Images/ab252432-393388-AntimtTFA-antibody-EPR23548120immunoprecipitationc2c12.jpg)
Immunoprecipitation - Anti-mtTFA antibody [EPR23548-120] (ab252432)mtTFA was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast) whole cell lysate using ab252432 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab252432 at 1/500 dilution, followed by VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution.
Lane 1: C2C12 whole cell lysate (Input)
Lane 2: ab252432 IP in C2C12 cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252432 in C2C12 whole cell lysateBlocking /Diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 32 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR23548-120] (ab252432)](https://www.abcam.com/ps/products/252/ab252432/Images/ab252432-2-anti-mttfa-antibody-epr23548120-immunocytochemistry-pc12.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR23548-120] (ab252432)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling mtTFA with ab252432 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.
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![Immunoprecipitation - Anti-mtTFA antibody [EPR23548-120] (ab252432)](https://www.abcam.com/ps/products/252/ab252432/Images/ab252432-393387-AntimtTFA-antibody-EPR23548120immunoprecipitationpc12lysate.jpg)
Immunoprecipitation - Anti-mtTFA antibody [EPR23548-120] (ab252432)mtTFA was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate using ab252432 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab252432 at 1/500 dilution, followed by VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution.
Lane 1: PC-12 whole cell lysate (Input)
Lane 2: ab252432 IP in PC-12 cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252432 in PC-12 whole cell lysateBlocking /Diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 32 seconds
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![Western blot - Anti-mtTFA antibody [EPR23548-120] (ab252432)](https://www.abcam.com/ps/products/252/ab252432/Images/ab252432-393115-anti-mttfa-antibody-epr23548120-western-blot-c2c12.jpg)
Western blot - Anti-mtTFA antibody [EPR23548-120] (ab252432)All lanes : Anti-mtTFA antibody [EPR23548-120] (ab252432) at 1/1000 dilution
Lane 1 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 2 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 3 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 25375380)
Exposure times: Lane 1: 3 minutes; Lanes 2-5: 70 seconds.
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![Anti-mtTFA antibody [EPR23548-120] (ab252432)](https://www.abcam.com/ps/products/252/ab252432/Images/ab252432-8-benefits-of-recombinant-antibodies.png)
Anti-mtTFA antibody [EPR23548-120] (ab252432)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (8)
ab252432 has been referenced in 8 publications.
- Chen JW et al. mito-TEMPO Attenuates Oxidative Stress and Mitochondrial Dysfunction in Noise-Induced Hearing Loss via Maintaining TFAM-mtDNA Interaction and Mitochondrial Biogenesis. Front Cell Neurosci 16:803718 (2022). PubMed: 35210991
- Yan M et al. Cajaninstilbene Acid Ameliorates Acetaminophen-Induced Liver Injury Through Enhancing Sestrin2/AMPK-Mediated Mitochondrial Quality Control. Front Pharmacol 13:824138 (2022). PubMed: 35350766
- Zhu Z et al. Photobiomodulation promotes repair following spinal cord injury by restoring neuronal mitochondrial bioenergetics via AMPK/PGC-1α/TFAM pathway. Front Pharmacol 13:991421 (2022). PubMed: 36172183
- Zhao W et al. Estrogen Deficiency Induces Mitochondrial Damage Prior to Emergence of Cognitive Deficits in a Postmenopausal Mouse Model. Front Aging Neurosci 13:713819 (2021). PubMed: 34335235
- Zhang Q et al. Exosomes from adipose-derived mesenchymal stem cells alleviate liver ischaemia reperfusion injury subsequent to hepatectomy in rats by regulating mitochondrial dynamics and biogenesis. J Cell Mol Med 25:10152-10163 (2021). PubMed: 34609057
- Wang Z et al. Chronic Restraint Stress-Induced Muscle Atrophy Leads to Fatigue in Mice by Inhibiting the AMPK Signaling Pathway. Biomedicines 9:N/A (2021). PubMed: 34680438
- Sun J et al. Nrf2 Activation Attenuates Chronic Constriction Injury-Induced Neuropathic Pain via Induction of PGC-1α-Mediated Mitochondrial Biogenesis in the Spinal Cord. Oxid Med Cell Longev 2021:9577874 (2021). PubMed: 34721761
- Yi Z et al. Activator-Mediated Pyruvate Kinase M2 Activation Contributes to Endotoxin Tolerance by Promoting Mitochondrial Biogenesis. Front Immunol 11:595316 (2020). PubMed: 33542713