Recombinant Anti-Mu Opioid Receptor antibody [UMB3] - Low endotoxin, Azide free (ab227067)
![Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - Low endotoxin, Azide free (ab227067)](https://www.abcam.com/ps/products/227/ab227067/Images/ab227067-288824-anti-mu-opioid-receptor-antibody-umb3-western-blot.jpg "Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - Low endotoxin, Azide free (ab227067)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UMB3] to Mu Opioid Receptor - Low endotoxin, Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Mu Opioid Receptor antibody [UMB3] - Low endotoxin, Azide free
See all Mu Opioid Receptor primary antibodies -
Description
Rabbit monoclonal [UMB3] to Mu Opioid Receptor - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Mu Opioid Receptor transfected HEK293 lysate.
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General notes
ab227067 is the carrier-free version of ab134054.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
UMB3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa).
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4988 Human
- Entrez Gene: 18390 Mouse
- Entrez Gene: 25601 Rat
- Omim: 600018 Human
- SwissProt: P35372 Human
- SwissProt: P42866 Mouse
- SwissProt: P33535 Rat
- Unigene: 2353 Human
see all -
Alternative names
- hMOP antibody
- LMOR antibody
- M-OR-1 antibody
see all
Images
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Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - Low endotoxin, Azide free (ab227067)All lanes : Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) at 1/1000 dilution
Lane 1 : Mu Opioid Receptor transfected HEK-293 (Human epithelial cell line from embryonic kidney) lysate
Lane 2 : HEK-293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
![Anti-Mu Opioid Receptor antibody [UMB3] - Low endotoxin, Azide free (ab227067)](https://www.abcam.com/ps/products/227/ab227067/Images/ab227067-2-benefits-of-recombinant-antibodies.png)
Anti-Mu Opioid Receptor antibody [UMB3] - Low endotoxin, Azide free (ab227067)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (9)
ab227067 has been referenced in 9 publications.
- Vatter T et al. Against Repurposing Methadone for Glioblastoma Therapy. Biomolecules 10:N/A (2020). PubMed: 32560384
- Crittenden JR et al. Striosome-dendron bouquets highlight a unique striatonigral circuit targeting dopamine-containing neurons. Proc Natl Acad Sci U S A 113:11318-11323 (2016). IHC-Fr ; Mouse . PubMed: 27647894
- Ozawa A et al. Knock-In Mice with NOP-eGFP Receptors Identify Receptor Cellular and Regional Localization. J Neurosci 35:11682-93 (2015). PubMed: 26290245
- Hassan B et al. Alteration of the mu opioid receptor: Ca2+ channel signaling pathway in a subset of rat sensory neurons following chronic femoral artery occlusion. J Neurophysiol 112:3104-15 (2014). PubMed: 25231620
- Just S et al. Differentiation of opioid drug effects by hierarchical multi-site phosphorylation. Mol Pharmacol 83:633-9 (2013). PubMed: 23239825
- Gallagher SK et al. Dopaminergic amacrine cells express opioid receptors in the mouse retina. Vis Neurosci 29:203-9 (2012). WB ; Mouse . PubMed: 22643230
- Lupp A et al. UMB-3, a novel rabbit monoclonal antibody, for assessing µ-opioid receptor expression in mouse, rat and human formalin-fixed and paraffin-embedded tissues. Regul Pept 167:9-13 (2011). PubMed: 20851148
- Doll C et al. Agonist-selective patterns of µ-opioid receptor phosphorylation revealed by phosphosite-specific antibodies. Br J Pharmacol 164:298-307 (2011). PubMed: 21449911
- Grecksch G et al. Analgesic tolerance to high-efficacy agonists but not to morphine is diminished in phosphorylation-deficient S375A µ-opioid receptor knock-in mice. J Neurosci 31:13890-6 (2011). IP, IHC-P ; Mouse . PubMed: 21957251
