Recombinant Anti-MUC4 antibody [EPR27199-56] (ab307546)
![Western blot - Anti-MUC4 antibody [EPR27199-56] (ab307546)](https://www.abcam.com/ps/products/307/ab307546/Images/ab307546-498271-anti-muc4-antibody-epr27199-56-western-blot-capan-1-human-pancreas-adenocarcinoma-epithelial-cell-whole-cell-lysate-20-ug.jpg "Western blot - Anti-MUC4 antibody [EPR27199-56] (ab307546)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27199-56] to MUC4
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MUC4 antibody [EPR27199-56]
See all MUC4 primary antibodies -
Description
Rabbit monoclonal [EPR27199-56] to MUC4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Capan-1 whole cell lysate. Human stomach tissue lysate. IHC-P: Human colon tissue. Human cervical carcinoma tissue. IP: Capan-1 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR27199-56 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab307546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 232 kDa.
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| IHC-P |
1/500.
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 232 kDa. |
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IHC-P
1/500. |
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IP
1/30. |
Target
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Function
May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. -
Tissue specificity
Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines. -
Sequence similarities
Contains 1 AMOP domain.
Contains 2 EGF-like domains.
Contains 1 NIDO domain.
Contains 1 VWFD domain. -
Developmental stage
Expressed early in the primitive gut before respiratory and digestive epithelial cells have acquired their tissue and cell specificity. Expressed at the basal surface of the epithelium from week 14 to 26 weeks and then predominantly localized in only parietal cells. Immediately before birth, found in the cytoplasm of the mucous columnar epithelial cells. In the embryo expressed in skin, then disappears late in gestation. -
Post-translational
modificationsProteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
mucrnin-4 alpha chain is highly O-glycosylated.
mucin-4 beta chain is predominantly N-glycosylated. -
Cellular localization
Secreted; Cell membrane and Membrane. Secreted. Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing. - Information by UniProt
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Database links
- Entrez Gene: 4585 Human
- Omim: 158372 Human
- SwissProt: Q99102 Human
- Unigene: 369646 Human
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Alternative names
- Ascites sialoglycoprotein 1 antibody
- Ascites sialoglycoprotein 2 antibody
- Ascites sialoglycoprotein antibody
see all
Images
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Western blot - Anti-MUC4 antibody [EPR27199-56] (ab307546)All lanes : Anti-MUC4 antibody [EPR27199-56] (ab307546) at 1/1000 dilution
Lane 1 : Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate 20 µg
Lane 2 : MIA PaCa-2 (human pancreas epithelial cell) whole cell lysate 20 µg
Lane 3 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 232 kDa
Observed band size: 100-170 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: MIA PaCa-2 (PMID: 21886786), PANC-1 (PMID: 23152882)
Exposure time: 147 seconds
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![Western blot - Anti-MUC4 antibody [EPR27199-56] (ab307546)](https://www.abcam.com/ps/products/307/ab307546/Images/ab307546-498272-anti-muc4-antibody-epr27199-56-western-blot-human-stomach-tissue-lysate-20-ug.jpg)
Western blot - Anti-MUC4 antibody [EPR27199-56] (ab307546)All lanes : Anti-MUC4 antibody [EPR27199-56] (ab307546) at 1/1000 dilution
Lane 1 : Human stomach tissue lysate 20 µg
Lane 2 : Human skeletal muscle cytoplasma tissue lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 232 kDa
Observed band size: 100-170 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle.
This blot was developed using a high sensitivity ECL substrate.
The identity of the bands below 75 kDa are unknown.
Exposure time: 180 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] (ab307546)](https://www.abcam.com/ps/products/307/ab307546/Images/ab307546-3-anti-muc4-antibody-epr27199-56-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] (ab307546)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MUC4 with ab307546 at 1/500 dilution (0.96 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human colon.
The section was incubated with ab307546 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] (ab307546)](https://www.abcam.com/ps/products/307/ab307546/Images/ab307546-4-anti-muc4-antibody-epr27199-56-immunohistochemistry-human-cervical-carci.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] (ab307546)Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling MUC4 with ab307546 at 1/500 dilution (0.96 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cervical carcinoma.
The section was incubated with ab307546 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] (ab307546)](https://www.abcam.com/ps/products/307/ab307546/Images/ab307546-5-anti-muc4-antibody-epr27199-56-immunohistochemistry-human-skeletal-muscl.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] (ab307546)Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling MUC4 with ab307546 at 1/500 dilution (0.96 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human skeletal muscle.
The section was incubated with ab307546 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunoprecipitation - Anti-MUC4 antibody [EPR27199-56] (ab307546)](https://www.abcam.com/ps/products/307/ab307546/Images/ab307546-2-anti-muc4-antibody-epr27199-56-immunoprecipitation-capan-1.jpg)
Immunoprecipitation - Anti-MUC4 antibody [EPR27199-56] (ab307546)MUC4 was immunoprecipitated from 0.35 mg Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab307546 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307546 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Capan-1 whole cell lysate 10 µg
Lane 2: ab307546 IP in Capan-1 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307546 in Capan-1 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 93 seconds.
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![Anti-MUC4 antibody [EPR27199-56] (ab307546)](https://www.abcam.com/ps/products/307/ab307546/Images/ab307546-1-benefitsofrecombinantantibodies.jpg)
Anti-MUC4 antibody [EPR27199-56] (ab307546)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab307546 has not yet been referenced specifically in any publications.