Recombinant Anti-Mucin 5AC antibody [45M1] (ab3649)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [45M1] to Mucin 5AC
- Suitable for: ICC/IF, IHC-Fr, mIHC, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Mucin 5AC antibody [45M1]
See all Mucin 5AC primary antibodies -
Description
Mouse monoclonal [45M1] to Mucin 5AC -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Fr, mIHC, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human, mouse, and rat stomach tissues; IHC-Fr: Mouse and rat stomach tissues; ICC/IF: A549 cells. mIHC: Human stomach tissue.
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General notes
This product has switched from a hybridoma to recombinant production method on 8th March 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
45M1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (2) |
1/100.
|
IHC-Fr | (2) |
1/100.
|
mIHC |
Use at an assay dependent concentration.
|
|
IHC-P | (3) |
1/100 - 1/5000.
|
Notes |
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ICC/IF
1/100. |
IHC-Fr
1/100. |
mIHC
Use at an assay dependent concentration. |
IHC-P
1/100 - 1/5000. |
Target
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Function
Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. -
Tissue specificity
Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 3 TIL (trypsin inhibitory-like) domains.
Contains 4 VWFC domains.
Contains 4 VWFD domains. -
Domain
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding. -
Post-translational
modificationsC-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis.
Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4586 Human
- Entrez Gene: 17833 Mouse
- Entrez Gene: 682837 Rat
- Omim: 158373 Human
- SwissProt: P98088 Human
- Unigene: 534332 Human
- Unigene: 558950 Human
- Unigene: 703588 Human
see all -
Alternative names
- Apomucin antibody
- Apomucin Major Airway Glycoprotein antibody
- Gastric mucin antibody
see all
Images
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Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Mucin 5AC (ab3649, gray; Opal™690), anti-MUC-6 (ab223846, green; Opal™520) and anti-Rab3D (ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Mucin 5AC stained on surface mucous cells. Panel D: anti-Rab3D stained on Chief cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab3649 (1/5000 dilution), ab223846 (1/1000 dilution), and ab128997 (1/10000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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Immunocytochemistry analysis of A549 (human lung carcinoma cell line) cells labeling Mucin 5AC with ab3649 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-beta Tubulin rabbit monoclonal antibody (ab179513) at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Negative control 1: ab3649 (Mouse monoclonal antibody to Mucin 5AC at 1/100 dilution) and ab150080 (anti-Rabbit secondary Alexa Fluor® 594 at 1/1000 dilution)
Negative control 2: ab179513 (Rabbit monoclonal antibody to beta Tubulin at 1/200 dilution) and ab150113 (anti-Mouse secondary Alexa Fluor® 488 at 1/1000 dilution)
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Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue labeling Mucin 5AC with ab3649 at 1/100 dilution. Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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Immunohistochemistry (Frozen sections) analysis of rat stomach tissue labeling Mucin 5AC with ab3649 at 1/100 dilution. Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)Image from Kikuchi M et al., BMC Gastroenterol. 2010 Jun 18;10:65. doi: 10.1186/1471-230X-10-65.; Fig 5.; 18 June 2010, BMC Gastroenterology 2010, 10:65
This image was generated from the hybridoma version of the product.
Immunohistochemical analysis of rat stomach tissue, staining Mucin 5AC with ab3649.
Antigen retrieval was performed via heat mediation in a citrate buffer. Samples were blocked before incubating with primary antibody (1/100) for 1 hour at room temperature. An HRP-conjugated anti-mouse IgG was used as the secondary antibody and staining was detected using DAB.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (113)
ab3649 has been referenced in 113 publications.
- Song G et al. Single-cell transcriptomic analysis suggests two molecularly subtypes of intrahepatic cholangiocarcinoma. Nat Commun 13:1642 (2022). PubMed: 35347134
- Kim S et al. Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids. Nat Commun 13:1692 (2022). PubMed: 35354790
- Gan Y et al. Estradiol inhibits autophagy of Mycobacterium tuberculosis‑infected 16HBE cells and controls the proliferation of intracellular Mycobacterium tuberculosis. Mol Med Rep 25:N/A (2022). PubMed: 35425995
- Arai J et al. Clinicopathological Features of Gastric Cancer with Autoimmune Gastritis. Biomedicines 10:N/A (2022). PubMed: 35453635
- Shibata O et al. Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method. Mol Ther Nucleic Acids 28:342-352 (2022). PubMed: 35474735