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RecombinantRabMAb

Recombinant Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)

Reviews (1) Submit a question References (40)
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6431(2)] to MURF1 + MURF3 + MURF2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)]

  • Description

    Rabbit monoclonal [EPR6431(2)] to MURF1 + MURF3 + MURF2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human MURF1 aa 1-100. The exact sequence is proprietary.
    Database link: Q969Q1

  • Positive control

    • Rat muscle, Mouse muscle and Human skeletal muscle tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 40 kDa.

Target

Images

  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Mouse muscle lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

    Lane 1 : Rat muscle tissue lysate
    Lane 2 : Mouse muscle tissue lysate
    Lane 3 : Human skeletal muscle tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa

  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF1 (aa 1 to 353) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF3 (full length) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/500 dilution + His-Tagged human MURF2 (full length) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 150 seconds
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/5000 dilution + Mouse muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 150 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

  • Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)

References (40)

Publishing research using ab172479? Please let us know so that we can cite the reference in this datasheet.

ab172479 has been referenced in 40 publications.

  • Zeng X  et al. Inhibition of mitochondrial and cytosolic calpain attenuates atrophy in myotubes co-cultured with colon carcinoma cells. Oncol Lett 21:124 (2021). PubMed: 33552245
  • Takegaki J  et al. The Effect of Leucine-Enriched Essential Amino Acid Supplementation on Anabolic and Catabolic Signaling in Human Skeletal Muscle after Acute Resistance Exercise: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Comparison Trial. Nutrients 12:N/A (2020). PubMed: 32806711
  • Koutnik AP  et al. Ketone Bodies Attenuate Wasting in Models of Atrophy. J Cachexia Sarcopenia Muscle 11:973-996 (2020). PubMed: 32239651
  • Wang D  et al. Baoyuan Jiedu Decoction Alleviates Cancer-Induced Myotube Atrophy by Regulating Mitochondrial Dynamics Through p38 MAPK/PGC-1a Signaling Pathway. Front Oncol 10:523577 (2020). PubMed: 33102208
  • Mose M  et al. A model mimicking catabolic inflammatory disease; a controlled randomized study in humans. PLoS One 15:e0241274 (2020). PubMed: 33151986
View all Publications for this product

Customer reviews and Q&As

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Western blot abreview for Anti-MURF1 antibody [EPR6431(2)]

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - other (muscle (cytosolic protein))
Gel Running Conditions
Reduced Denaturing (9)
Loading amount
50 µg
Specification
muscle (cytosolic protein)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Baptiste Jude

Verified customer

Submitted Oct 13 2016

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