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  1. Link

    products/primary-antibodies/musashi-1--msi1-antibody-y2709-ab129819.pdf

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Neuroscience Cell Type Marker Neural Stem Cell marker
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Validated using a knockout cell line

Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)
  • Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)
  • Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)

Key features and details

  • Mouse monoclonal [Y2709] to Musashi 1 / Msi1
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-Musashi 1 / Msi1 antibody [Y2709]
    See all Musashi 1 / Msi1 primary antibodies
  • Description

    Mouse monoclonal [Y2709] to Musashi 1 / Msi1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    corresponding to Musashi 1/ Msi1.

  • Positive control

    • WB: human brain membrane; E14 rat embryonic brain; SHSY5Y whole cell; 46C whole cell. ICC/IF: HAP1-MSI1 cells
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    Y2709
  • Isotype

    IgG2a
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Cytoplasmic
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Nuclear
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Stem Cells
    • Neural Stem Cells
    • Glial Restricted Lineage
    • Astrocyte

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • Tissue specificity

    Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
  • Sequence similarities

    Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain

    The first RNA recognition motif binds more strongly to RNA compared to the second one.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession O43347 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4440 Human
    • Entrez Gene: 17690 Mouse
    • Entrez Gene: 259272 Rat
    • Omim: 603328 Human
    • SwissProt: O43347 Human
    • SwissProt: Q61474 Mouse
    • SwissProt: Q8K3P4 Rat
    • Unigene: 158311 Human
    • Unigene: 5077 Mouse
    • Unigene: 162146 Rat
    see all
  • Alternative names

    • Msi 1 antibody
    • Msi1 antibody
    • MSI1H_HUMAN antibody
    • Musashi homolog 1 antibody
    • Musashi RNA binding protein 1 antibody
    • Musashi-1 antibody
    • Musashi1 antibody
    • RNA binding protein Musashi homolog 1 antibody
    • RNA-binding protein Musashi homolog 1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)
    Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)

    ab129819 staining Musashi 1 / Msi1 in HAP1-MSI1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab129819 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)
    Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)
    All lanes : Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/ml

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : MSI1 knockout HAP1 whole cell lysate
    Lane 3 : SH-SY5Y whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab129819 observed at 39 kDa. Red - loading control, ab52866, observed at 50 kDa.

    ab129819 was shown to specifically react with Musashi 1 / Msi1 in wild-type HAP1 cells as signal was lost in MSI1 knockout cells. Wild-type and MSI1 knockout samples were subjected to SDS-PAGE. Ab129819 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 5 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)
    Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)
    All lanes : Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/ml

    Lane 1 : Brain (Human) Membrane Lysate - fetal normal tissue (ab29457)
    Lane 2 : E14 Rat Embryo Brain Tissue Lysate
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 4 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 12 minutes

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab129819? Please let us know so that we can cite the reference in this datasheet.

ab129819 has been referenced in 1 publication.

  • Kuwako K  et al. Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression. Neuron 67:407-21 (2010). IP . PubMed: 20696379

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