Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody (ab77098)
Key features and details
- Goat polyclonal to Muscarinic Acetylcholine Receptor 1/CHRM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody -
Description
Goat polyclonal to Muscarinic Acetylcholine Receptor 1/CHRM1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Muscarinic Acetylcholine Receptor 1/CHRM1 aa 438-453 (C terminal).
Sequence:C-DKRRWRKIPKRPGSVH
Database link: NP_000729.2 -
Positive control
- Human brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab77098 is purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 51 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 51 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Relevance
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. Muscarinic receptors are so called as they are more sensitive to the effects of the alkaloid muscarine, rather than nicotine. -
Cellular localization
Cell Membrane; multi-pass membrane protein -
Database links
- Entrez Gene: 1128 Human
- Entrez Gene: 12669 Mouse
- Entrez Gene: 397099 Pig
- Entrez Gene: 25229 Rat
- Omim: 118510 Human
- SwissProt: P11229 Human
- SwissProt: P12657 Mouse
- SwissProt: Q5R949 Orangutan
see all -
Alternative names
- AChR antibody
- Cholinergic receptor muscarinic 1 antibody
- CHRM1 antibody
see all
Images
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Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody (ab77098) at 0.300000011920929 µg/ml + human brain lysate (in RIPA buffer) at 35 µg
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (2)
ab77098 has been referenced in 2 publications.
- Tsolias A & Medalla M Muscarinic Acetylcholine Receptor Localization on Distinct Excitatory and Inhibitory Neurons Within the ACC and LPFC of the Rhesus Monkey. Front Neural Circuits 15:795325 (2021). PubMed: 35087381
- Winder M et al. Autonomic Receptor-mediated Regulation of Production and Release of Nitric Oxide in Normal and Malignant Human Urothelial Cells. Basic Clin Pharmacol Toxicol 121:257-265 (2017). PubMed: 28437032