Recombinant Anti-Myc tag antibody [9E10] (ab206486)
![Western blot - Anti-Myc tag antibody [9E10] (ab206486)](https://www.abcam.com/ps/products/206/ab206486/Images/ab206486-481244-anti-myc-tag-antibody-9e10-western-blot-wildtype-hek293t-myc-knockout-hek293t-jurkat-shsy5y-ab.jpg "Western blot - Anti-Myc tag antibody [9E10] (ab206486)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [9E10] to Myc tag
- Suitable for: IHC-Fr, IHC-P, ICC/IF, IP, WB
Overview
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Product name
Anti-Myc tag antibody [9E10]
See all Myc tag primary antibodies -
Description
Rat monoclonal [9E10] to Myc tag -
Host species
Rat -
Specificity
This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC/IF, IP, WBmore details -
Immunogen
Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus (C terminal).
Database link: P01106 -
Epitope
Epitope located at aa 410-419; EQKLISEEDL -
General notes
ab206486 is a recombinant antibody that comprises the variable regions from the 9E10 hybridoma within a rat IgG1 scaffold.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
9E10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- DyLight® 650 Anti-Myc tag antibody [9E10] (ab117487)
- DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
- DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
- FITC Anti-Myc tag antibody [9E10] (ab117599)
- Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
- Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
- Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
- Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
- Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
- Anti-Myc tag antibody [9E10] (ab32)
- HRP Anti-Myc tag antibody [9E10] (ab62928)
- PE Anti-Myc tag antibody [9E10] (ab72468)
- SureLight® APC Anti-Myc tag antibody [9E10] (ab72580)
- Biotin Anti-Myc tag antibody [9E10] (ab81658)
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Assay kits
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| IHC-P |
Use at an assay dependent concentration.
|
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| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| IP | (1) |
Use at an assay dependent concentration.
|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa. |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Images
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Western blot - Anti-Myc tag antibody [9E10] (ab206486)All lanes : Anti-Myc tag antibody [9E10] (ab32) at 1/200 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : MYC CRISPR-Cas9 edited HEK-293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDaFalse colour image of Western blot: Anti-Myc tag antibody [9E10] staining at 1/200 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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![Western blot - Anti-Myc tag antibody [9E10] (ab206486)](https://www.abcam.com/ps/products/206/ab206486/Images/ab206486-356997-anti-myc-tag-antibody-9e10-westernblot-tagged-protein.png)
Western blot - Anti-Myc tag antibody [9E10] (ab206486)All lanes : Anti-Myc tag antibody [9E10] (ab206486) at 2 µg/ml
Lane 1 : HEK-293 spiked with 5 ng multitag protein
Lane 2 : HEK-293 unspiked with 5 ng multitag protein
Lane 3 : 25 ng purified multitag protein
Lysates/proteins at 35 µg per lane.
Predicted band size: 48 kDa -
![Western blot - Anti-Myc tag antibody [9E10] (ab206486)](https://www.abcam.com/ps/products/206/ab206486/Images/ab206486-356996-anti-myc-tag-antibody-9e10-westernblot-tagged-protein-2.png)
Western blot - Anti-Myc tag antibody [9E10] (ab206486)All lanes : Anti-Myc tag antibody [9E10] (ab206486) at 1 µg/ml
Lane 1 : Multitag protein at 0.025 µg
Lane 2 : Multitag protein at 0.01 µg
Lane 3 : Multitag protein at 0.005 µg
Predicted band size: 48 kDa -
![Anti-Myc tag antibody [9E10] (ab206486)](https://www.abcam.com/ps/products/206/ab206486/Images/ab206486-3-benefits-of-recombinant-antibodies.png)
Anti-Myc tag antibody [9E10] (ab206486)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab206486 has been referenced in 9 publications.
- Karabiyik C et al. Glucose starvation induces autophagy via ULK1-mediated activation of PIKfyve in an AMPK-dependent manner. Dev Cell 56:1961-1975.e5 (2021). PubMed: 34107300
- Zeng J et al. Inflammation and DNA methylation coregulate the CtBP-PCAF-c-MYC transcriptional complex to activate the expression of a long non-coding RNA CASC2 in acute pancreatitis. Int J Biol Sci 16:2116-2130 (2020). PubMed: 32549759
- Yu CH et al. TDP-43 Triggers Mitochondrial DNA Release via mPTP to Activate cGAS/STING in ALS. Cell 183:636-649.e18 (2020). PubMed: 33031745
- Compte M et al. A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity. Nat Commun 9:4809 (2018). PubMed: 30442944
- Ferro M et al. Functional mapping of brain synapses by the enriching activity-marker SynaptoZip. Nat Commun 8:1229 (2017). PubMed: 29089485
- Gali VK et al. Translesion synthesis DNA polymerase ? exhibits a specific RNA extension activity and a transcription-associated function. Sci Rep 7:13055 (2017). PubMed: 29026143
- Alonso-Camino V et al. CARbodies: Human Antibodies Against Cell Surface Tumor Antigens Selected From Repertoires Displayed on T Cell Chimeric Antigen Receptors. Mol Ther Nucleic Acids 2:e93 (2013). PubMed: 23695536
- Liu H et al. Degradation of regulator of calcineurin 1 (RCAN1) is mediated by both chaperone-mediated autophagy and ubiquitin proteasome pathways. FASEB J 23:3383-92 (2009). PubMed: 19509306
- Lin M et al. Functional expression of a biologically active fragment of soluble gp130 as an ELP-fusion protein in transgenic plants: purification via inverse transition cycling. Biochem J 398:577-83 (2006). PubMed: 16716147