Anti-Myc tag antibody (ab9106)
Key features and details
- Rabbit polyclonal to Myc tag
- Suitable for: IHC-Fr, IP, WB, IHC-P, ICC/IF, Electron Microscopy
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Myc tag antibody
See all Myc tag primary antibodies -
Description
Rabbit polyclonal to Myc tag -
Host species
Rabbit -
Specificity
ELISA: Antibody specificity was verified by ELISA against the peptide (EQKLISEEDL). A 1:60,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP-conjugated Goat Anti Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run. -
Tested applications
Suitable for: IHC-Fr, IP, WB, IHC-P, ICC/IF, Electron Microscopymore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to Myc tag conjugated to keyhole limpet haemocyanin.
(Peptide available asab13837) -
Positive control
- ICC/IF: CHO cells transfected with 12-tags constructs. IHC-P: Mouse brain tissue stained for Myc tag. EM: Mouse embryonic fibroblasts expressing myc-tagged protein. IP: Huh7 expressing myc-tagged CDC42 protein and transfected 293FT cells. WB: Y. enterocolitica ?HOPEMT carrying plasmids encoding Myc-tagged CT082 or CT695, and HA-tagged Slc1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
1/500.
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| IP | (9) |
Use at an assay dependent concentration.
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| WB | (14) |
Use at an assay dependent concentration.
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| IHC-P | (3) |
Use at an assay dependent concentration.
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| ICC/IF | (10) |
1/1000.
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| Electron Microscopy |
Use at an assay dependent concentration. See Abreview for further details (submitted by Eeva-Liisa Eskelinen).
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| Notes |
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IHC-Fr
1/500. |
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
1/1000. |
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Electron Microscopy
Use at an assay dependent concentration. See Abreview for further details (submitted by Eeva-Liisa Eskelinen). |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (ab9106)
Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilized with 0.1% Triton X-100 in CHO cells transfected with 12-tags constructs (named as CHO-12 tags, Top) and parental CHO cells (Bottom) labelling MYC with ab9106 at 1/1000 dilution (1 μg/mL), followed by Donkey Anti-rabbit IgG (H&L), FITC conjugated antibody at 1/1000 dilution (1 μg/mL) (Green). Nucleus was counterstained with DAPI (Blue).
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Western blot - Anti-Myc tag antibody (ab9106)Pais et al PLoS One. 2013;8(2):e56292. doi: 10.1371/journal.pone.0056292. Epub 2013 Feb 19. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Y. enterocolitica ΔHOPEMT carrying plasmids encoding Myc-tagged CT082 or CT695, and HA-tagged Slc1 (as indicated) were grown in non-secreting conditions. Chloramphenicol was added (time?=?0 min) to stop bacterial protein synthesis and samples were taken at the indicated time points. Samples were analyzed by immunoblotting using ab9106, anti-HA, and anti-TEM antibodies.
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Immunoprecipitation - Anti-Myc tag antibody (ab9106)This image is courtesy of an anonymous abreview.ab9106 at 4 µg/mg lysate used in Huh7 (Human cell line) whole cell lysate (1x106 cells).
Cells expressed myc-tagged CDC42 protein. Immunoprecipitation step performed using Protein A matrix. Incubation time 4 hours at 4°C. Western Blot antibody used at 1/300 dilution.
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Immunoprecipitation - Anti-Myc tag antibody (ab9106)ab9106 used at 1/250 in the immunoprecipitation of transfected human 293FT cells (whole cell lysate).
In this experiment cells were co-transfected with myc tagged cdc25 along with an HA tagged viral protein which binds to cdc25. The cell lysate was immunoprecipitated using ab9106, run on a 10% gel. The blot was probed for HA and HA tagged protein (which co-IPed with Myc tagged protein) and this is seen at ~15KDa.
Lane 1: Input non-transfected cells
Lane 2: Immunoprecipitated non-transfected cells
Lane 3: Input co-transfected cells
Lane 4: Immunoprecipitated co-transfected cells
This image is courtesy of an Abreview submitted on 23 March 2006.
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Immunohistochemistry (Frozen sections) - Anti-Myc tag antibody (ab9106)This image is courtesy of an anonymous abreview.Paraformaldehyde-fixed frozen mouse brain tissue stained for Myc tag using ab9106 at a 1/500 dilution (2hrs at 25°C), followed by a goat anti-rabbit Alexa-Fluor® 568 secondary used at a 1/500 dilution.
For further details please see abreview.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myc tag antibody (ab9106)This image is courtesy of Karen Lee by Abreview.Paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized mouse brain tissue stained for Myc tag using ab9106 at a 1/500 dilution (15 hrs, 4°C) followed by and Alexa-Fluor® 555 donkey anti-rabbit secondary used at a 1/500 dilution.
For further details please see abreview.
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Electron Microscopy - Anti-Myc tag antibody (ab9106)This image is courtesy of an Abreview submitted by Dr Eeva-Liisa Eskelinenab9106 at 1/2000 staining mouse embryonic fibroblasts expressing myc-tagged protein by ICC.
The cells were paraformaldehyde fixed and blocked with 3% BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit IgG antibody (10 nm gold conjugated) was used as the secondary.
The image shown is immuno electron microscopical staining with thawed cryosections.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (472)
ab9106 has been referenced in 472 publications.
- Yuan L et al. The ubiquitin ligase RNF2 stabilizes ERα and modulates breast cancer progression. Hum Cell 36:353-365 (2023). PubMed: 36271315
- Binda O et al. SMA-linked SMN mutants prevent phase separation properties and SMN interactions with FMRP family members. Life Sci Alliance 6:N/A (2023). PubMed: 36375840
- Liu F et al. circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis. Cancers (Basel) 14:N/A (2022). PubMed: 36497344
- Yang H et al. RNF31 represses cell progression and immune evasion via YAP/PD-L1 suppression in triple negative breast Cancer. J Exp Clin Cancer Res 41:364 (2022). PubMed: 36581998
- Barber KW et al. CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling. Cell Rep Methods 2:100318 (2022). PubMed: 36313802