For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/myc-tag-antibody-ab9106.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Epitope Tags Myc Tag
Share by email

Anti-Myc tag antibody (ab9106)

  • Datasheet
  • SDS
Reviews (39)Q&A (11)References (415)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Myc tag antibody (ab9106)
  • Immunoprecipitation - Anti-Myc tag antibody (ab9106)
  • Immunoprecipitation - Anti-Myc tag antibody (ab9106)
  • Immunohistochemistry (Frozen sections) - Anti-Myc tag antibody (ab9106)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myc tag antibody (ab9106)
  • Electron Microscopy - Anti-Myc tag antibody (ab9106)

Key features and details

  • Rabbit polyclonal to Myc tag
  • Suitable for: IHC-Fr, IP, WB, IHC-P, ICC, Electron Microscopy
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Myc tag antibody [9E10] (ab206486)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Myc tag antibody
    See all Myc tag primary antibodies
  • Description

    Rabbit polyclonal to Myc tag
  • Host species

    Rabbit
  • Specificity

    ELISA: Antibody specificity was verified by ELISA against the peptide (EQKLISEEDL). A 1:60,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP-conjugated Goat Anti Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run.
  • Tested applications

    Suitable for: IHC-Fr, IP, WB, IHC-P, ICC, Electron Microscopymore details
  • Immunogen

    Synthetic peptide corresponding to Myc tag conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab13837)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg of IgG.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Tumor biomarkers
    • Oncoproteins

Associated products

  • Assay kits

    • c-Myc Transcription Factor Assay Kit (Colorimetric) (ab207200)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-Myc tag antibody [9E10] (ab32)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
1/500.
IP (9)
Use at an assay dependent concentration.
WB (14)
Use at an assay dependent concentration.
IHC-P (3)
Use at an assay dependent concentration.
ICC (2)
Use at an assay dependent concentration.
Electron Microscopy
Use at an assay dependent concentration. See Abreview for further details (submitted by Eeva-Liisa Eskelinen).
Notes
IHC-Fr
1/500.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Electron Microscopy
Use at an assay dependent concentration. See Abreview for further details (submitted by Eeva-Liisa Eskelinen).

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization

    Nuclear
  • Alternative names

    • c-myc tag antibody
    • Myc Epitope Tag antibody

Images

  • Western blot - Anti-Myc tag antibody (ab9106)
    Western blot - Anti-Myc tag antibody (ab9106)Pais et al PLoS One. 2013;8(2):e56292. doi: 10.1371/journal.pone.0056292. Epub 2013 Feb 19. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Y. enterocolitica ΔHOPEMT carrying plasmids encoding Myc-tagged CT082 or CT695, and HA-tagged Slc1 (as indicated) were grown in non-secreting conditions. Chloramphenicol was added (time?=?0 min) to stop bacterial protein synthesis and samples were taken at the indicated time points. Samples were analyzed by immunoblotting using ab9106, anti-HA, and anti-TEM antibodies.

  • Immunoprecipitation - Anti-Myc tag antibody (ab9106)
    Immunoprecipitation - Anti-Myc tag antibody (ab9106)This image is courtesy of an anonymous abreview.

    ab9106 at 4 µg/mg lysate used in Huh7 (Human cell line) whole cell lysate (1x106 cells).

    Cells expressed myc-tagged CDC42 protein. Immunoprecipitation step performed using Protein A matrix. Incubation time 4 hours at 4°C. Western Blot antibody used at 1/300 dilution.

    See Abreview

  • Immunoprecipitation - Anti-Myc tag antibody (ab9106)
    Immunoprecipitation - Anti-Myc tag antibody (ab9106)

    ab9106 used at 1/250 in the immunoprecipitation of transfected human 293FT cells (whole cell lysate).

    In this experiment cells were co-transfected with myc tagged cdc25 along with an HA tagged viral protein which binds to cdc25. The cell lysate was immunoprecipitated using ab9106, run on a 10% gel. The blot was probed for HA and HA tagged protein (which co-IPed with Myc tagged protein) and this is seen at ~15KDa.

    Lane 1: Input non-transfected cells

    Lane 2: Immunoprecipitated non-transfected cells

    Lane 3: Input co-transfected cells

    Lane 4: Immunoprecipitated co-transfected cells

    This image is courtesy of an Abreview submitted on 23 March 2006.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Myc tag antibody (ab9106)
    Immunohistochemistry (Frozen sections) - Anti-Myc tag antibody (ab9106)This image is courtesy of an anonymous abreview.

    Paraformaldehyde-fixed frozen mouse brain tissue stained for Myc tag using ab9106 at a 1/500 dilution (2hrs at 25°C), followed by a goat anti-rabbit Alexa-Fluor® 568 secondary used at a 1/500 dilution.

    For further details please see abreview.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myc tag antibody (ab9106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myc tag antibody (ab9106)This image is courtesy of Karen Lee by Abreview.

    Paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized mouse brain tissue stained for Myc tag using ab9106 at a 1/500 dilution (15 hrs, 4°C) followed by and Alexa-Fluor® 555 donkey anti-rabbit secondary used at a 1/500 dilution.

    For further details please see abreview.

    See Abreview

  • Electron Microscopy - Anti-Myc tag antibody (ab9106)
    Electron Microscopy - Anti-Myc tag antibody (ab9106)This image is courtesy of an Abreview submitted by Dr Eeva-Liisa Eskelinen

    ab9106 at 1/2000 staining mouse embryonic fibroblasts expressing myc-tagged protein by ICC.

    The cells were paraformaldehyde fixed and blocked with 3% BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit IgG antibody (10 nm gold conjugated) was used as the secondary.

    The image shown is immuno electron microscopical staining with thawed cryosections.

    See Abreview

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (415)

Publishing research using ab9106? Please let us know so that we can cite the reference in this datasheet.

ab9106 has been referenced in 415 publications.

  • Cui X  et al. Mechanism of TREM2/DAP12 complex affecting ß-amyloid plaque deposition in Alzheimer's disease modeled mice through mediating inflammatory response. Brain Res Bull 166:21-28 (2021). PubMed: 33053435
  • Yang Z  et al. HDAC3-dependent transcriptional repression of FOXA2 regulates FTO/m6A/MYC signaling to contribute to the development of gastric cancer. Cancer Gene Ther 28:141-155 (2021). PubMed: 32655129
  • Lee SH  et al. RUNX3 methylation drives hypoxia-induced cell proliferation and antiapoptosis in early tumorigenesis. Cell Death Differ 28:1251-1269 (2021). PubMed: 33116296
  • Yin XK  et al. PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer. Oncogene 40:1375-1389 (2021). PubMed: 33420374
  • Bauer S  et al. The leucine-rich repeats in allelic barley MLA immune receptors define specificity towards sequence-unrelated powdery mildew avirulence effectors with a predicted common RNase-like fold. PLoS Pathog 17:e1009223 (2021). PubMed: 33534797
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 50 Abreviews or Q&A

Western blot abreview for Anti-Myc tag antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Treatment
transfected with myc-tagged protein expressing plasmid
Specification
HEK cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 02 2019

Western blot abreview for Anti-Myc tag antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
3e+006 cells
Treatment
Transfected with 10µg of expression vector encoding a Myc-fused protein.
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 16 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myc tag antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - Triton X-100
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 16 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myc tag antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human microglial cell line)
Permeabilization
Yes - 0.1% triton X-100
Specification
human microglial cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 09 2018

Western blot abreview for Anti-Myc tag antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Treatment
Cells tranfected with empty vector or myc-tagged SIRT1 followed by IP SIRT1.
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 06 2017

Western blot abreview for Anti-Myc tag antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293 Cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
Transfected with Myc-GFP plasmid.
Specification
293 Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 19 2015

Western blot abreview for Anti-Myc tag antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Specification
hepatocytes
Treatment
lipofectamine 2000 transfected
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 10 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myc tag antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
0.3% BSA/8%donkeyserum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Karen Yee

Verified customer

Submitted Oct 02 2014

Western blot abreview for Anti-Myc tag antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat, K562, and HL-60 cells)
Loading amount
50000 cells
Specification
Jurkat, K562, and HL-60 cells
Gel Running Conditions
Reduced Denaturing (4-20% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 21 2013

Western blot abreview for Anti-Myc tag antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Tobacco Tissue lysate - whole (leaf)
Loading amount
20 µg
Specification
leaf
Gel Running Conditions
Non-reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 28 2013

1-10 of 50 Abreviews or Q&A

  •  Previous
  • 1
  • 2
  • 3
  • 4
  • 5
  • Next 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.