Anti-Myc tag antibody - ChIP Grade (ab9132)
Key features and details
- Goat polyclonal to Myc tag
- Suitable for: IP, ChIP, WB, ICC, ELISA
- Isotype: IgG
Overview
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Product name
Anti-Myc tag antibody - ChIP Grade
See all Myc tag primary antibodies -
Description
Goat polyclonal to Myc tag -
Host species
Goat -
Specificity
Antibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA). -
Tested applications
Suitable for: IP, ChIP, WB, ICC, ELISAmore details -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.80
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at 1-4 µg/mg of lysate.
Also PubMed: 20224777
|
|
| ChIP | (2) |
Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.
|
| WB | (3) |
1/1000 - 1/30000.
|
| ICC |
1/200 - 1/2000.
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| ELISA |
1/100 - 1/500.
|
| Notes |
|---|
|
IP
Use at 1-4 µg/mg of lysate. Also PubMed: 20224777
|
|
ChIP
Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. |
|
WB
1/1000 - 1/30000. |
|
ICC
1/200 - 1/2000. |
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ELISA
1/100 - 1/500. |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Images
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Western blot - Anti-Myc tag antibody - ChIP Grade (ab9132)All lanes : Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate at 0.2 µg
Lane 2 : E. coli whole cell lysate at 0.1 µg
Lane 3 : E. coli whole cell lysate at 0.05 µg -
ChIP - Anti-Myc tag antibody - ChIP Grade (ab9132)A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (143)
ab9132 has been referenced in 143 publications.
- Higerd-Rusli GP et al. The fates of internalized NaV1.7 channels in sensory neurons: Retrograde cotransport with other ion channels, axon-specific recycling, and degradation. J Biol Chem 299:102816 (2023). PubMed: 36539035
- Laut CL et al. DnaJ and ClpX Are Required for HitRS and HssRS Two-Component System Signaling in Bacillus anthracis. Infect Immun 90:e0056021 (2022). PubMed: 34748369
- Turn RE et al. The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and cilia to regulate ciliogenesis and ciliary protein traffic. Mol Biol Cell 33:ar13 (2022). PubMed: 34818063
- Wang X et al. Input and output organization of the mesodiencephalic junction for cerebro-cerebellar communication. J Neurosci Res 100:620-637 (2022). PubMed: 34850425
- Søreng K et al. ALFY localizes to early endosomes and cellular protrusions to facilitate directional cell migration. J Cell Sci 135:N/A (2022). PubMed: 35099014