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    products/primary-antibodies/myelin-basic-protein-antibody-ab40390.pdf

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Neuroscience Cell Adhesion Proteins Membrane Proteins
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Anti-Myelin Basic Protein antibody (ab40390)

  • Datasheet
  • SDS
Reviews (20)Q&A (3)References (186)

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Immunocytochemistry - Anti-Myelin Basic Protein antibody (ab40390)
  • Western blot - Anti-Myelin Basic Protein antibody (ab40390)
  • Immunocytochemistry - Anti-Myelin Basic Protein antibody (ab40390)

Key features and details

  • Rabbit polyclonal to Myelin Basic Protein
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Myelin Basic Protein antibody [IGX3421] (ab209328)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Myelin Basic Protein antibody
    See all Myelin Basic Protein primary antibodies
  • Description

    Rabbit polyclonal to Myelin Basic Protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Myelin Basic Protein aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: P04370
    (Peptide available as ab40389)

  • General notes

    For a recombinant version of Myelin Basic Protein antibody (WB, ICC/IF, IHC-P, Human, Mouse, Rat) - see ab209328 - clone IGX3421.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Mouse Myelin Basic Protein peptide (ab40389)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Myelin Basic Protein (Tagged) (ab43614)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (6)
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.Can be blocked with Mouse Myelin Basic Protein peptide (ab40389).
ICC
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.Can be blocked with Mouse Myelin Basic Protein peptide (ab40389).
ICC
Use a concentration of 1 µg/ml.

Target

  • Function

    The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificity

    MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in disease

    Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similarities

    Belongs to the myelin basic protein family.
  • Developmental stage

    Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    modifications

    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localization

    Myelin membrane. Cytoplasmic side of myelin.
  • Target information above from: UniProt accession P02686 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 17196 Mouse
    • Entrez Gene: 24547 Rat
    • SwissProt: P04370 Mouse
    • SwissProt: P02688 Rat
    • Unigene: 252063 Mouse
    • Unigene: 392350 Mouse
    • Unigene: 454459 Mouse
    • Alternative names

      • GDB antibody
      • Golli MBP antibody
      • Golli MBP; myelin basic protein antibody
      • Hemopoietic MBP antibody
      • HMBPR antibody
      • HUGO antibody
      • MBP antibody
      • MBP_CAVPO antibody
      • MBP_HUMAN antibody
      • MGC99675 antibody
      • MLD antibody
      • Myelin A1 protein antibody
      • Myelin A1 Protein, basic antibody
      • Myelin basic protein antibody
      • Myelin Deficient antibody
      • Myelin membrane encephalitogenic protein antibody
      • OTTHUMP00000163776 antibody
      • OTTHUMP00000174387 antibody
      • OTTHUMP00000174388 antibody
      • SHI antibody
      • Shiverer antibody
      • SP antibody
      see all

    Images

    • Immunocytochemistry - Anti-Myelin Basic Protein antibody (ab40390)
      Immunocytochemistry - Anti-Myelin Basic Protein antibody (ab40390)

      ab40390 staining Myelin Basic Protein in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab40390 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 4% paraformaldehyde (10 min).

      Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Western blot - Anti-Myelin Basic Protein antibody (ab40390)
      Western blot - Anti-Myelin Basic Protein antibody (ab40390)
      All lanes : Anti-Myelin Basic Protein antibody (ab40390) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Brain (Rat) Tissue Lysate - normal tissue

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 33 kDa
      Observed band size: 18,23 kDa why is the actual band size different from the predicted?
      Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.



      This antibody was raised against an immunogen that is predicted to recognize isoforms (5, 7, 8, 10 and 13) of Myelin Basic Protein (MBP). The predicted molecular weights of isoforms (5, 7, 8, 10 and 13) are 18.5kDa, 17kDa, 14kDa, 21kDa and 13kDa respectively.
    • Immunocytochemistry - Anti-Myelin Basic Protein antibody (ab40390)
      Immunocytochemistry - Anti-Myelin Basic Protein antibody (ab40390)
      ICC/IF image of ab40390 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40390, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (186)

    Publishing research using ab40390? Please let us know so that we can cite the reference in this datasheet.

    ab40390 has been referenced in 186 publications.

    • Patkar OL  et al. Analysis of homozygous and heterozygous Csf1r knockout in the rat as a model for understanding microglial function in brain development and the impacts of human CSF1R mutations. Neurobiol Dis 151:105268 (2021). PubMed: 33450391
    • Wan B  et al. GIT1 protects traumatically injured spinal cord by prompting microvascular endothelial cells to clear myelin debris. Aging (Albany NY) 13:7067-7083 (2021). PubMed: 33621952
    • Feng Y  et al. Dl-3-n-Butylphthalide Alleviates Demyelination and Improves Cognitive Function by Promoting Mitochondrial Dynamics in White Matter Lesions. Front Aging Neurosci 13:632374 (2021). PubMed: 33762923
    • Hsiao MY  et al. Pathogenic Hydrogel? A Novel-Entrapment Neuropathy Model Induced by Ultrasound-Guided Perineural Injections. Int J Mol Sci 22:N/A (2021). PubMed: 33800600
    • Liu Y  et al. Myelin Debris Stimulates NG2/CSPG4 Expression in Bone Marrow-Derived Macrophages in the Injured Spinal Cord. Front Cell Neurosci 15:651827 (2021). PubMed: 33815067
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 23 Abreviews or Q&A

    Western blot abreview for Anti-Myelin Basic Protein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver and brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    50 µg
    Specification
    liver and brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 12 2020

    Western blot abreview for Anti-Myelin Basic Protein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver and brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    50 µg
    Specification
    liver and brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 09 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myelin Basic Protein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (C22 mouse sciatic nerve paraffin-embedded section)
    Permeabilization
    No
    Specification
    C22 mouse sciatic nerve paraffin-embedded section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 22 2019

    Western blot abreview for Anti-Myelin Basic Protein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (cerebellar lysate)
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Loading amount
    10 µg
    Specification
    cerebellar lysate
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Maria Velasco

    Verified customer

    Submitted Nov 28 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Myelin Basic Protein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain(Hippocampus))
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% Triton X
    Specification
    Brain(Hippocampus)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. J. Y. Seo

    Verified customer

    Submitted Apr 09 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Myelin Basic Protein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brain)
    Antigen retrieval step
    None
    Specification
    brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. Francoise Geffroy

    Verified customer

    Submitted Apr 07 2018

    Western blot abreview for Anti-Myelin Basic Protein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (4%-20%)
    Loading amount
    10 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Sandra Braz

    Verified customer

    Submitted Aug 02 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myelin Basic Protein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Sensory neuronal co-culture)
    Permeabilization
    Yes - 0.5% Triton
    Specification
    Sensory neuronal co-culture
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 26 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin Basic Protein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections ( Mouse Tissue sections -Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Permeabilization
    Yes - 0.1% Tween 20 in PBS
    Specification
     Mouse Tissue sections -Brain
    Blocking step
    Serum as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Chia-Li Lin

    Verified customer

    Submitted Nov 23 2015

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Myelin Basic Protein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Horse Serum in 0.3% Triton X-100-PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Antigen retrieval step
    None
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Brandon Jun

    Verified customer

    Submitted Dec 17 2014

    1-10 of 23 Abreviews or Q&A

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