Recombinant Anti-Myelin PLP antibody [plpc 1] (ab9311)

Samples are non-boiled as boiling may cause protein aggregates.

The molecular weight observed is consistent with what has been described in the literature (PMID: 9247276).

Negative control: liver (PMID: 2414013).

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.

The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.

The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.

The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Myelin PLP with ab9311 at 1/25 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1:1000 dilution (Green). is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.