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RecombinantRabMAb

Recombinant Anti-Myeloperoxidase antibody [EPR17996] (ab188211)

Submit a review Submit a question References (4)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
  • Western blot - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
  • Western blot - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
  • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
  • Anti-Myeloperoxidase antibody [EPR17996] (ab188211)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17996] to Myeloperoxidase
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-Myeloperoxidase antibody [EPR17996]
    See all Myeloperoxidase primary antibodies

  • Description

    Rabbit monoclonal [EPR17996] to Myeloperoxidase

  • Host species

    Rabbit

  • Specificity

    This antibody is specific to Myeloperoxidase light chain.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat spleen lysates. IHC-P: Human, mouse and rat spleen tissues. Flow Cyt (intra): Mouse PBMC.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 89, 74, 13 kDa (predicted molecular weight: 83 kDa).
IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 89, 74, 13 kDa (predicted molecular weight: 83 kDa).
IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

  • Involvement in disease

    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.

  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.

  • Cellular localization

    Lysosome.
  • Information by UniProt

  • Database links

    • Alternative names

      • 84 kDa myeloperoxidase antibody
      • 89 kDa myeloperoxidase antibody
      • EC 1.11.1.7 antibody
      • EC1.11.2.2 antibody
      • fj80f04 antibody
      • MPO antibody
      • mpx antibody
      • myeloid-specific peroxidase antibody
      • Myeloperoxidase antibody
      • Myeloperoxidase heavy chain antibody
      • Myeloperoxidase light chain antibody
      • PERM_HUMAN antibody
      • wu:fj80f04 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of human spleen [PMID: 19566938].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of mouse spleen [PMID: 19566938].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)

      Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab188211 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neutrophils of rat spleen [PMID: 19566938].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
      Western blot - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
      All lanes : Anti-Myeloperoxidase antibody [EPR17996] (ab188211) at 1/1000 dilution

      Lane 1 : Mouse spleen lysate
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 83 kDa
      Observed band size: 13,74,89 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature. PMID: 2154223. 89 kDa (MPO), 74 kDa (intermediate form), 13 kDa (light chain)

      Negative control: NIH/3T3  PMID: 9001423.

    • Western blot - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
      Western blot - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
      Anti-Myeloperoxidase antibody [EPR17996] (ab188211) at 1/1000 dilution + Rat spleen lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 83 kDa
      Observed band size: 13,74 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature PMID: 2154223.

    • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
      Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR17996] (ab188211)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed mouse PBMC labeling Myeloperoxidase with ab188211 at 1/500 dilution (Right) compared with a rabbit monoclonal IgG isotype control (ab172730; Left). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

      Mouse peripheral blood mononuclear cells stained intracellularly with ab188211 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged. 

       

       

    • Anti-Myeloperoxidase antibody [EPR17996] (ab188211)
      Anti-Myeloperoxidase antibody [EPR17996] (ab188211)

    References (4)

    Publishing research using ab188211? Please let us know so that we can cite the reference in this datasheet.

    ab188211 has been referenced in 4 publications.

    • Samain R  et al. Pharmacologic Normalization of Pancreatic Cancer-Associated Fibroblast Secretome Impairs Prometastatic Cross-Talk With Macrophages. Cell Mol Gastroenterol Hepatol 11:1405-1436 (2021). PubMed: 33482394
    • Zhang SR  et al. IL-37 increases in patients after ischemic stroke and protects from inflammatory brain injury, motor impairment and lung infection in mice. Sci Rep 9:6922 (2019). PubMed: 31061403
    • Lee JS  et al. PCSK9 inhibition as a novel therapeutic target for alcoholic liver disease. Sci Rep 9:17167 (2019). PubMed: 31748600
    • Tian X  et al. Protective Role of Nuclear Factor-Erythroid 2-Related Factor 2 Against Radiation-Induced Lung Injury and Inflammation. Front Oncol 8:542 (2018). PubMed: 30533397

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