Name: Anti-Myeloperoxidase antibody [EPR20257]
Brand: RabMAb
SKU: ab208670
Rating: 5 (6 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20257] to Myeloperoxidase
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 APC Carrier Free

Product name

Anti-Myeloperoxidase antibody [EPR20257]
See all Myeloperoxidase primary antibodies
Description

Rabbit monoclonal [EPR20257] to Myeloperoxidase
Host species

Rabbit
Specificity

This antibody is specific to Myeloperoxidase heavy chain.
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal spleen lysate; Rat and mouse spleen lysates; HL-60 whole cell lysate. IHC-P: Human spleen and stomach cancer tissues; Mouse and rat spleen tissues. ICC/IF: HL-60 cells. Flow Cyt (intra): Mouse PBMC and HL-60 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20257
Isotype

IgG
Research areas

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab208670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/500.
WB		1/1000. Detects a band of approximately 89, 59 kDa (predicted molecular weight: 83 kDa).
IHC-P	(3)	1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/100.

Notes
Flow Cyt (Intra) 1/500.
WB 1/1000. Detects a band of approximately 89, 59 kDa (predicted molecular weight: 83 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Function

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Involvement in disease

Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
Sequence similarities

Belongs to the peroxidase family. XPO subfamily.
Cellular localization

Lysosome.
Target information above from: UniProt accession P05164 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4353 Human
- Entrez Gene: 17523 Mouse
- Entrez Gene: 303413 Rat
- Omim: 606989 Human
- SwissProt: P05164 Human
- SwissProt: P11247 Mouse
- Unigene: 458272 Human
- Unigene: 4668 Mouse
Alternative names
- 84 kDa myeloperoxidase antibody
- 89 kDa myeloperoxidase antibody
- EC 1.11.1.7 antibody
- EC1.11.2.2 antibody
- fj80f04 antibody
- MPO antibody
- mpx antibody
- myeloid-specific peroxidase antibody
- Myeloperoxidase antibody
- Myeloperoxidase heavy chain antibody
- Myeloperoxidase light chain antibody
- PERM_HUMAN antibody
- wu:fj80f04 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of human spleen is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

All lanes : Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/1000 dilution

Lane 1 : Human fetal spleen lysate
Lane 2 : Rat spleen lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID: 3029127 PMID: 2154223.

Negative control: HeLa PMID 12040446.
Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunofluorescent analysis of 100% methanol-fixed HL-60 (Human promyelocytic leukemia cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Myeloperoxidase with ab208670 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on HL-60 cell line.

Negative control: HeLa (PMID: 12040446).

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of human stomach cancer is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa cells (left panel) and HL-60cells (right panel)labeling Myeloperoxidase with ab208670 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control ( (ab172730);black)andunlabelled control (cells without incubation with primary and secondary antibodies; blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HeLa (PMID: 12040446).
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/5000 dilution + Mouse spleen lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID: 3029127 PMID: 2154223.
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/20000 dilution + HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 39,59,89 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID: 2154223 PMID: 8384653.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of mouse spleen is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of rat spleen is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Mouse PBMC cells labeling Myeloperoxidase with ab208670 at 1/500 dilution (right), compared with a rabbit monoclonal IgG isotype control (ab172730;left). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody. Mouse peripheral blood mononuclear cells stained intracellularly with ab208670 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.
Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab208670? Please let us know so that we can cite the reference in this datasheet.

ab208670 has been referenced in 95 publications.

Zhu H et al. Comparison of intratumor and local immune response between MV X-ray FLASH and conventional radiotherapies. Clin Transl Radiat Oncol 38:138-146 (2023). PubMed: 36425537
Zhang ZT et al. Dexmedetomidine alleviates acute lung injury by promoting Tregs differentiation via activation of AMPK/SIRT1 pathway. Inflammopharmacology 31:423-438 (2023). PubMed: 36534240
Shi K et al. Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo. Int J Mol Sci 23:N/A (2022). PubMed: 36555773
Chen Q et al. Prognostic value of tumor-associated N1/N2 neutrophil plasticity in patients following radical resection of pancreas ductal adenocarcinoma. J Immunother Cancer 10:N/A (2022). PubMed: 36600557
Pawlik K et al. Pharmacological Evidence of the Important Roles of CCR1 and CCR3 and Their Endogenous Ligands CCL2/7/8 in Hypersensitivity Based on a Murine Model of Neuropathic Pain. Cells 12:N/A (2022). PubMed: 36611891

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1-6 of 6 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Dog Tissue sections (spleen & colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9

Permeabilization

Specification

spleen & colon

Blocking step

(agent) for 10 minute(s) · Concentration: 100% · Temperature: 21°C

Fixative

10% NBF

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 26 2022

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Spleen)

Permeabilization

Specification

Spleen

Blocking step

10% Normal Serum, 1% BSA in TBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Fixative

Methanol

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Brett Bell

Verified customer

Submitted Oct 20 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Liver)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0

Permeabilization

Specification

Liver

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 01 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Connective tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate

Permeabilization

Yes - Triton

Specification

Connective tissue

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tina Yuan

Verified customer

Submitted Jan 05 2020

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (connective tissue)

Permeabilization

Yes - Triton

Specification

connective tissue

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tina Yuan

Verified customer

Submitted Jan 05 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Purified neutrophils)

Permeabilization

Yes - 0.1% Triton

Specification

Purified neutrophils

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 11 2018

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Assay kits

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (95)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myeloperoxidase antibody [EPR20257]

Immunohistochemistry (Frozen sections) abreview for Anti-Myeloperoxidase antibody [EPR20257]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myeloperoxidase antibody [EPR20257]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myeloperoxidase antibody [EPR20257]

Immunohistochemistry (Frozen sections) abreview for Anti-Myeloperoxidase antibody [EPR20257]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myeloperoxidase antibody [EPR20257]