Recombinant Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20257] to Myeloperoxidase
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Myeloperoxidase antibody [EPR20257]
See all Myeloperoxidase primary antibodies -
Description
Rabbit monoclonal [EPR20257] to Myeloperoxidase -
Host species
Rabbit -
Specificity
This antibody is specific to Myeloperoxidase heavy chain. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal spleen lysate; Rat and mouse spleen lysates; HL-60 whole cell lysate. IHC-P: Human spleen and stomach cancer tissues; Mouse and rat spleen tissues. ICC/IF: HL-60 cells. Flow Cyt (intra): Mouse PBMC and HL-60 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20257 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
- Alexa Fluor® 488 Anti-Myeloperoxidase antibody [EPR20257] (ab225474)
- APC Anti-Myeloperoxidase antibody [EPR20257] (ab225476)
- Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a - Chimeric (ab300650)
- Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a - Chimeric - BSA and Azide Free (ab300651)
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/500.
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WB |
1/1000. Detects a band of approximately 89, 59 kDa (predicted molecular weight: 83 kDa).
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IHC-P | (3) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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Notes |
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Flow Cyt (Intra)
1/500. |
WB
1/1000. Detects a band of approximately 89, 59 kDa (predicted molecular weight: 83 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Target
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Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 4353 Human
- Entrez Gene: 17523 Mouse
- Entrez Gene: 303413 Rat
- Omim: 606989 Human
- SwissProt: P05164 Human
- SwissProt: P11247 Mouse
- Unigene: 458272 Human
- Unigene: 4668 Mouse
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Alternative names
- 84 kDa myeloperoxidase antibody
- 89 kDa myeloperoxidase antibody
- EC 1.11.1.7 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of human spleen is observed [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/1000 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : Rat spleen lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID: 3029127 PMID: 2154223.
Negative control: HeLa PMID 12040446.
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Immunofluorescent analysis of 100% methanol-fixed HL-60 (Human promyelocytic leukemia cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Myeloperoxidase with ab208670 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HL-60 cell line.
Negative control: HeLa (PMID: 12040446).
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of human stomach cancer is observed [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa cells (left panel) and HL-60cells (right panel)labeling Myeloperoxidase with ab208670 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control ( (ab172730);black)andunlabelled control (cells without incubation with primary and secondary antibodies; blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HeLa (PMID: 12040446).
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Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/5000 dilution + Mouse spleen lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID: 3029127 PMID: 2154223.
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Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/20000 dilution + HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 39,59,89 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature. PMID: 2154223 PMID: 8384653.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of mouse spleen is observed [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of rat spleen is observed [PMID: 19566938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Mouse PBMC cells labeling Myeloperoxidase with ab208670 at 1/500 dilution (right), compared with a rabbit monoclonal IgG isotype control (ab172730;left). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Mouse peripheral blood mononuclear cells stained intracellularly with ab208670 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (95)
ab208670 has been referenced in 95 publications.
- Zhu H et al. Comparison of intratumor and local immune response between MV X-ray FLASH and conventional radiotherapies. Clin Transl Radiat Oncol 38:138-146 (2023). PubMed: 36425537
- Zhang ZT et al. Dexmedetomidine alleviates acute lung injury by promoting Tregs differentiation via activation of AMPK/SIRT1 pathway. Inflammopharmacology 31:423-438 (2023). PubMed: 36534240
- Shi K et al. Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo. Int J Mol Sci 23:N/A (2022). PubMed: 36555773
- Chen Q et al. Prognostic value of tumor-associated N1/N2 neutrophil plasticity in patients following radical resection of pancreas ductal adenocarcinoma. J Immunother Cancer 10:N/A (2022). PubMed: 36600557
- Pawlik K et al. Pharmacological Evidence of the Important Roles of CCR1 and CCR3 and Their Endogenous Ligands CCL2/7/8 in Hypersensitivity Based on a Murine Model of Neuropathic Pain. Cells 12:N/A (2022). PubMed: 36611891