Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
  • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
  • Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
  • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
  • Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20257] to Myeloperoxidase - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 APC Unconjugated

Overview

  • Product name

    Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free
    See all Myeloperoxidase primary antibodies

  • Description

    Rabbit monoclonal [EPR20257] to Myeloperoxidase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab221847 is the carrier-free version of ab208670.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab221847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 89, 59 kDa (predicted molecular weight: 83 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 89, 59 kDa (predicted molecular weight: 83 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

  • Involvement in disease

    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.

  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.

  • Cellular localization

    Lysosome.
  • Information by UniProt

  • Database links

    • Alternative names

      • 84 kDa myeloperoxidase antibody
      • 89 kDa myeloperoxidase antibody
      • EC 1.11.1.7 antibody
      • EC1.11.2.2 antibody
      • fj80f04 antibody
      • MPO antibody
      • mpx antibody
      • myeloid-specific peroxidase antibody
      • Myeloperoxidase antibody
      • Myeloperoxidase heavy chain antibody
      • Myeloperoxidase light chain antibody
      • PERM_HUMAN antibody
      • wu:fj80f04 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasmic staining on neutrophils of human spleen is observed [PMID: 19566938].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

    • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
      Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

       Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa cells (left panel) and HL-60 cells (right panel) labeling  Myeloperoxidase with ab208670 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control ( (ab172730); black) and unlabelled control (cells without incubation with primary and secondary antibodies; blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.   Negative control: HeLa (PMID: 12040446). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

      Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasmic staining on neutrophils of human stomach cancer is observed [PMID: 19566938].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasmic staining on neutrophils of mouse spleen is observed [PMID: 19566938].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

      Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasmic staining on neutrophils of rat spleen is observed [PMID: 19566938].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

    • Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
      Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

      Immunofluorescent analysis of 100% methanol-fixed HL-60 (Human promyelocytic leukemia cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Myeloperoxidase with ab208670 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic staining on HL-60 cell line.

      Negative control: HeLa (PMID: 12040446).

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

    • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
      Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

       Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Mouse PBMC cells labeling  Myeloperoxidase with ab208670 at 1/500 dilution (right), compared with a rabbit monoclonal IgG isotype control (ab172730; left). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.   Mouse peripheral blood mononuclear cells stained intracellularly with ab208670 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670). 

       

       

    • Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
      Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab221847? Please let us know so that we can cite the reference in this datasheet.

    ab221847 has been referenced in 1 publication.

    • McDowell SAC  et al. Neutrophil oxidative stress mediates obesity-associated vascular dysfunction and metastatic transmigration. Nat Cancer 2:545-562 (2021). PubMed: 35122017

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab221847.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com