Recombinant Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a - Chimeric (ab300650)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with AB300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).
Positive staining on human spleen.
The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling Myeloperoxidase with AB300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).
Positive staining on human gastric carcinoma.
The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with AB300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).
Positive staining on mouse spleen.
The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with AB300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).
Positive staining on rat spleen.
The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HL-60 (human acute promyelocytic leukemia promyeloblast) cells labeling Myeloperoxidase with AB300650 at 1/50 dilution (20.26 µg/ml), followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
Confocal image showing cytoplasmic staining in HL-60 cell line.
Negative control: HeLa (PMID: 12040446).
ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution (4 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).
-ve control 1: AB300650 at a 1/50 dilution followed by ab150080 at a 1/500 dilution.
-ve control 2: ab179513 at a 1/200 dilution followed by ab150161 at a 1/1000 dilution.

Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left) / HL-60 (human acute promyelocytic leukemia promyeloblast, Right) cells labeling Myeloperoxidase with AB300650 at 1/1000 dilution (0.1µg) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor^® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Negative control: HeLa (PMID: 12040446).

Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized mouse blood cells labeling Myeloperoxidase with AB300650 at 1/1000 dilution (0.1µg)/ Right compared with a rat monoclonal IgG / Left. Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor^® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.