Recombinant Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
![Western blot - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)](https://www.abcam.com/ps/products/109/ab109116/Images/ab109116-121693-anti-myeloperoxidase-antibody-epr4792-western-blot.jpg "Western blot - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4792] to Myeloperoxidase
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Myeloperoxidase antibody [EPR4792]
See all Myeloperoxidase primary antibodies -
Description
Rabbit monoclonal [EPR4792] to Myeloperoxidase -
Host species
Rabbit -
Specificity
ab109116 recognizes Myeloperoxidase (85-90 kDa) and its light chain (15 kDa). -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HL-60 cell lysate IHC-P: Human colon, lung and spleen tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4792 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
|
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 84 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 4353 Human
- Omim: 606989 Human
- SwissProt: P05164 Human
- Unigene: 458272 Human
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Alternative names
- 84 kDa myeloperoxidase antibody
- 89 kDa myeloperoxidase antibody
- EC 1.11.1.7 antibody
see all
Images
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Western blot - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)Anti-Myeloperoxidase antibody [EPR4792] (ab109116) at 1/1000 dilution + HL-60 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 85-90 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa (possible cleavage fragment) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)](https://www.abcam.com/ps/products/109/ab109116/Images/ab109116-121692-anti-myeloperoxidase-antibody-epr4792-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)](https://www.abcam.com/ps/products/109/ab109116/Images/ab109116-121691-anti-myeloperoxidase-antibody-epr4792-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human lung tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)](https://www.abcam.com/ps/products/109/ab109116/Images/ab109116-121690-anti-myeloperoxidase-antibody-epr4792-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human spleen tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)](https://www.abcam.com/ps/products/109/ab109116/Images/ab109116-121689-anti-myeloperoxidase-antibody-epr4792-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)ab109116 showing positive staining in Normal liver tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)](https://www.abcam.com/ps/products/109/ab109116/Images/ab109116-121688-anti-myeloperoxidase-antibody-epr4792-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)ab109116 showing positive staining in Bone marrow tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)](https://www.abcam.com/ps/products/109/ab109116/Images/ab109116-121687-anti-myeloperoxidase-antibody-epr4792-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)ab109116 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Myeloperoxidase antibody [EPR4792] (ab109116)](https://www.abcam.com/ps/products/109/ab109116/Images/ab109116-8-benefits-of-recombinant-antibodies.png)
Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109116 has been referenced in 2 publications.
- Khamwong M et al. Burkholderia pseudomallei biofilm phenotypes confined but surviving in neutrophil extracellular traps of varying appearance. Front Immunol 13:926788 (2022). PubMed: 36059509
- Baek JH et al. Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin. Toxins (Basel) 6:1244-59 (2014). WB, ICC/IF ; Guinea pig . PubMed: 24691127