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    products/primary-antibodies/myeloperoxidase-antibody-sp72-ab93665.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-Myeloperoxidase antibody [SP72] (ab93665)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [SP72] (ab93665)
  • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [SP72] (ab93665)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [SP72] (ab93665)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [SP72] (ab93665)
  • Anti-Myeloperoxidase antibody [SP72] (ab93665)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP72] to Myeloperoxidase
  • Suitable for: IHC-P, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 APC Carrier Free HRP PE

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Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136)
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Recombinant Human Myeloperoxidase protein (ab158915)
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Human c-Myc peptide (ab166837)

View more associated products

Overview

  • Product name

    Anti-Myeloperoxidase antibody [SP72]
    See all Myeloperoxidase primary antibodies
  • Description

    Rabbit monoclonal [SP72] to Myeloperoxidase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt (Intra)more details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Myeloperoxidase aa 600-700 (C terminal). The exact sequence is proprietary.
    Database link: P05164

  • Epitope

    C terminus
  • Positive control

    • IHC: human tonsil and human spleen tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    SP72
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Other
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Arterial
    • Cardiovascular
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    • Antioxidants
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-Myeloperoxidase antibody [SP72] - BSA and Azide free (ab236218)
    • Alexa Fluor® 647 Anti-Myeloperoxidase antibody [SP72] (ab279686)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Myeloperoxidase protein (ab158915)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab93665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100.

30 minutes at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
IHC-P
1/100.

30 minutes at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease

    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization

    Lysosome.
  • Target information above from: UniProt accession P05164 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4353 Human
    • Omim: 606989 Human
    • SwissProt: P05164 Human
    • Unigene: 458272 Human
    • Alternative names

      • 84 kDa myeloperoxidase antibody
      • 89 kDa myeloperoxidase antibody
      • EC 1.11.1.7 antibody
      • EC1.11.2.2 antibody
      • fj80f04 antibody
      • MPO antibody
      • mpx antibody
      • myeloid-specific peroxidase antibody
      • Myeloperoxidase antibody
      • Myeloperoxidase heavy chain antibody
      • Myeloperoxidase light chain antibody
      • PERM_HUMAN antibody
      • wu:fj80f04 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [SP72] (ab93665)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [SP72] (ab93665)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling Myeloperoxidase with ab93665 at 1/00 dilution (2.49 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human spleen, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab93665 for 30 mins at room temperature.

    • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [SP72] (ab93665)
      Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [SP72] (ab93665)

      Overlay histogram showing HeLa cells stained with ab93665 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93665, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [SP72] (ab93665)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [SP72] (ab93665)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil
      tissue sections labeling Myeloperoxidase with ab93665 at 1/00 dilution (2.49 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically positive staining on human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab93665 for 30 mins at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [SP72] (ab93665)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [SP72] (ab93665)
      ab93665, at a 1/100 dilution, staining Myeloperoxidase in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry.
    • Anti-Myeloperoxidase antibody [SP72] (ab93665)
      Anti-Myeloperoxidase antibody [SP72] (ab93665)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab93665? Please let us know so that we can cite the reference in this datasheet.

    ab93665 has been referenced in 1 publication.

    • Weckbach LT  et al. Midkine drives cardiac inflammation by promoting neutrophil trafficking and NETosis in myocarditis. J Exp Med 216:350-368 (2019). PubMed: 30647120

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