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RecombinantRabMAb

Recombinant Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)

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Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
  • Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10891(2)] to MYH6 + Slow Skeletal Myosin Heavy chain - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR10891(2)] to MYH6 + Slow Skeletal Myosin Heavy chain - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 78%,76%,76%,76%,72%homology with Myosin 2 1 3 8 4, respectively. Cross-reactivity with these proteins has not been confirmed experimentally.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His tagged recombinant Human Myosin-7 and 6 protein, mouse and rat heart lysates. IHC: Human skeletal muscle

  • General notes

    ab250868 is the carrier-free version of ab185967.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 224 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 224 kDa).

Target

Images

  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Lane 1 : Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/1000 dilution
    Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/1000 dilution (His Tag)

    All lanes : His tagged recombinant Human Myosin-7 protein(aa 1191-1335)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 224 kDa



    ab185967 could recognize both Myosin 6 and 7 protein

  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Lane 1 : Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/1000 dilution
    Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/1000 dilution

    All lanes : His tagged recombinant Human Myosin-6 protein(aa 1193-1337)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated)

    Predicted band size: 224 kDa



    ab185967 could recognize both Myosin 6 and 7 protein

  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    All lanes : Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/500 dilution

    Lane 1 : Human adult heart lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IR680 at 1/10000 dilution

    Predicted band size: 224 kDa



    This data was developed using ab185967, the same antibody clone in a different buffer formulation.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab185967 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)

    This data was developed using ab185967, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain with ab185967 at 1/2000 dilution. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)
    Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free (ab250868)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab250868? Please let us know so that we can cite the reference in this datasheet.

ab250868 has not yet been referenced specifically in any publications.

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