Name: Anti-MYH7B antibody [EPR12290]
Brand: RabMAb
SKU: ab172967
Rating: 4 (3 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12290] to MYH7B
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-MYH7B antibody [EPR12290]
See all MYH7B primary antibodies
Description

Rabbit monoclonal [EPR12290] to MYH7B
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal muscle, fetal heart and skeletal muscle lysates. IHC-P: Human skeletal muscle, cardiac muscle, pancreas and heart tissues. ICC/IF: Rat glial tumor cell line.
General notes
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12290
Isotype

IgG
Research areas

Alternative Versions
- Anti-MYH7B antibody [EPR12290] - BSA and Azide free (ab240173)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/1000 - 1/5000. Predicted molecular weight: 221 kDa.
IHC-P	(1)	1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified format use at 1/250 - 1/500.

Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 221 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified format use at 1/250 - 1/500.

Function

Involved in muscle contraction.
Tissue specificity

Expressed in heart, skeletal muscle, testis, and all specific brain regions examined. Slightly lower expression was detected in ovary and kidney, and intermediate expression was detected in lung, pancreas, spleen and liver.
Sequence similarities

Contains 1 IQ domain.
Contains 1 myosin head-like domain.
Developmental stage

Found in fetal liver and brain.
Cellular localization

Membrane.
Target information above from: UniProt accession A7E2Y1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 57644 Human
- SwissProt: A7E2Y1 Human
- SwissProt: A2AQP0 Mouse
- Unigene: 414122 Human
Alternative names
- Antigen MLAA-21 antibody
- cardiac muscle beta isoform antibody
- Myh7b antibody
- MYH7B_HUMAN antibody
- Myosin cardiac muscle beta chain antibody
- Myosin heavy chain 7B antibody
- Myosin-7B antibody
- Slow A MYH14 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling MYH7B with ab172967 at a 1/250 dilution.
Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)

Anti-MYH7B antibody [EPR12290] (ab172967) at 0.05 µg/ml (Purified) + Human fetal muscle lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 221 kDa
Observed band size: 221 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)

All lanes : Anti-MYH7B antibody [EPR12290] (ab172967) at 0.1 µg/ml (Purified)

Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 221 kDa
Observed band size: 221 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

ab172967 showing +ve staining in Human normal pancreas tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling MYH7B with ab172967 at a 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

ab172967 showing -ve staining in Human normal liver tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)

ab172967 showing -ve staining in Human normal breast tissue.
Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)

All lanes : Anti-MYH7B antibody [EPR12290] (ab172967) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal muscle lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Human skeletal muscle lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 221 kDa
Anti-MYH7B antibody [EPR12290] (ab172967)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab172967? Please let us know so that we can cite the reference in this datasheet.

ab172967 has been referenced in 13 publications.

Huo S et al. Alleviation of Inflammation and Oxidative Stress in Pressure Overload-Induced Cardiac Remodeling and Heart Failure via IL-6/STAT3 Inhibition by Raloxifene. Oxid Med Cell Longev 2021:6699054 (2021). PubMed: 33824698
Barbeau PA et al. Ablating the Rab-GTPase activating protein TBC1D1 predisposes rats to high-fat diet-induced cardiomyopathy. J Physiol 598:683-697 (2020). PubMed: 31845331
Park SJ et al. Dual stem cell therapy synergistically improves cardiac function and vascular regeneration following myocardial infarction. Nat Commun 10:3123 (2019). PubMed: 31311935
Kar S et al. Hydrogen Sulfide Ameliorates Homocysteine-Induced Cardiac Remodeling and Dysfunction. Front Physiol 10:598 (2019). PubMed: 31178749
Kar S et al. Exercise Training Promotes Cardiac Hydrogen Sulfide Biosynthesis and Mitigates Pyroptosis to Prevent High-Fat Diet-Induced Diabetic Cardiomyopathy. Antioxidants (Basel) 8:N/A (2019). PubMed: 31835893

View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Skeletal muscle)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0

Permeabilization

Specification

Skeletal muscle

Blocking step

(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Helder Fonseca

Verified customer

Submitted Aug 17 2022

Application

Immunohistochemistry (Frozen sections)

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Sample

Human Tissue sections (heart)

Specification

heart

Permeabilization

Yes - Triton x-100, 0.01%

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Shyam Sundar Nandi

Verified customer

Submitted Nov 20 2014

Application

Western blot

Loading amount

40 µg

Gel Running Conditions

Reduced Denaturing (10% PAGE)

Sample

Human Cell lysate - whole cell (heart)

Specification

heart

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Shyam Sundar Nandi

Verified customer

Submitted Nov 19 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Developmental stage

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (13)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MYH7B antibody [EPR12290]

Immunohistochemistry (Frozen sections) abreview for Anti-MYH7B antibody [EPR12290]

Western blot abreview for Anti-MYH7B antibody [EPR12290]