Recombinant Anti-MYH7B antibody [EPR12290] (ab172967)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12290] to MYH7B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MYH7B antibody [EPR12290]
See all MYH7B primary antibodies -
Description
Rabbit monoclonal [EPR12290] to MYH7B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal muscle, fetal heart and skeletal muscle lysates. IHC-P: Human skeletal muscle, cardiac muscle, pancreas and heart tissues. ICC/IF: Rat glial tumor cell line.
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General notes
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12290 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 221 kDa.
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IHC-P | (1) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified format use at 1/250 - 1/500. |
Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 221 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified format use at 1/250 - 1/500. |
Target
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Function
Involved in muscle contraction. -
Tissue specificity
Expressed in heart, skeletal muscle, testis, and all specific brain regions examined. Slightly lower expression was detected in ovary and kidney, and intermediate expression was detected in lung, pancreas, spleen and liver. -
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
Developmental stage
Found in fetal liver and brain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 57644 Human
- SwissProt: A7E2Y1 Human
- SwissProt: A2AQP0 Mouse
- Unigene: 414122 Human
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Alternative names
- Antigen MLAA-21 antibody
- cardiac muscle beta isoform antibody
- Myh7b antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded Human heart tissue labeling MYH7B with ab172967 at a 1/250 dilution.
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Anti-MYH7B antibody [EPR12290] (ab172967) at 0.05 µg/ml (Purified) + Human fetal muscle lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 221 kDa
Observed band size: 221 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-MYH7B antibody [EPR12290] (ab172967) at 0.1 µg/ml (Purified)
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 221 kDa
Observed band size: 221 kDaBlocking and diluting buffer: 5% NFDM/TBST
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ab172967 showing +ve staining in Human normal pancreas tissue.
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Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling MYH7B with ab172967 at a 1/250 dilution.
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ab172967 showing -ve staining in Human normal liver tissue.
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ab172967 showing -ve staining in Human normal breast tissue.
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All lanes : Anti-MYH7B antibody [EPR12290] (ab172967) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal muscle lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Human skeletal muscle lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 221 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab172967 has been referenced in 17 publications.
- Yang Y et al. The lncRNA MIAT regulates CPT-1a mediated cardiac hypertrophy through m6A RNA methylation reading protein Ythdf2. Cell Death Discov 8:167 (2022). PubMed: 35383152
- Benkner A et al. Riociguat attenuates the changes in left ventricular proteome and microRNA profile after experimental aortic stenosis in mice. Br J Pharmacol 179:4575-4592 (2022). PubMed: 35751875
- Svedlund Eriksson E et al. Castration of Male Mice Induces Metabolic Remodeling of the Heart. J Endocr Soc 6:bvac132 (2022). PubMed: 36249410
- Yang Y et al. Oxytocin Protects Against Isoproterenol-Induced Cardiac Hypertrophy by Inhibiting PI3K/AKT Pathway via a lncRNA GAS5/miR-375-3p/KLF4-Dependent Mechanism. Front Pharmacol 12:766024 (2021). PubMed: 34925023
- Huo S et al. Alleviation of Inflammation and Oxidative Stress in Pressure Overload-Induced Cardiac Remodeling and Heart Failure via IL-6/STAT3 Inhibition by Raloxifene. Oxid Med Cell Longev 2021:6699054 (2021). PubMed: 33824698