Anti-MYO5A antibody (ab244249)
Key features and details
- Rabbit polyclonal to MYO5A
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MYO5A antibody
See all MYO5A primary antibodies -
Description
Rabbit polyclonal to MYO5A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Chicken -
Immunogen
Recombinant fragment corresponding to Human MYO5A aa 1350-1550.
Database link: Q9Y4I1 -
Positive control
- ICC/IF: A431 cells, human cerebral cortex. cerebellum and hippocampus tissue IHC-P: Human cerebellum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation. -
Tissue specificity
Detected in melanocytes. -
Involvement in disease
Griscelli syndrome 1
Griscelli syndrome 3
Elejalde syndrome -
Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
Contains 1 dilute domain.
Contains 6 IQ domains.
Contains 1 myosin motor domain. - Information by UniProt
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Database links
- Entrez Gene: 4644 Human
- Entrez Gene: 25017 Rat
- Omim: 160777 Human
- SwissProt: Q02440 Chicken
- SwissProt: Q9Y4I1 Human
- SwissProt: Q9QYF3 Rat
- Unigene: 21213 Human
- Unigene: 596221 Human
see all -
Alternative names
- non-muscle antibody
- Dilute myosin heavy chain antibody
- GS1 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human liver tissue stained for MYO5A with ab244249 at a 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for MYO5A with ab244249 at a 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human hippocampus tissue stained for MYO5A with ab244249 at a 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cerebellum tissue stained for MYO5A with ab244249 at a 1/200 dilution in immunohistochemical analysis.
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PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling MYO5A using ab244249 at 4 µg/ml (green) in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab244249 has not yet been referenced specifically in any publications.