Recombinant Anti-MyoD1 antibody [EPR6653-131] (ab133627)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-147518-anti-myod1-antibody-epr6653-131-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6653-131] to MyoD1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-MyoD1 antibody [EPR6653-131]
See all MyoD1 primary antibodies -
Description
Rabbit monoclonal [EPR6653-131] to MyoD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MyoD1 aa 50-150.
Database link: P15172 -
Positive control
- IHC-P: Human Rhabdomyosarcoma tissue WB: Human Rhabdomyosarcoma Cell Line Rh30, RD (Human muscle rhabdomyosarcoma) whole cell lysate ICC/IF: RD (Human muscle rhabdomyosarcoma) cell line.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6653-131 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Assay kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
Abcam recommends using milk as the blocking agent. |
|
| ICC/IF |
1/100.
|
|
| IHC-P | (5) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). Abcam recommends using milk as the blocking agent. |
|
ICC/IF
1/100. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 4654 Human
- Omim: 159970 Human
- SwissProt: P15172 Human
- Unigene: 181768 Human
-
Alternative names
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
Immunohistochemical analysis of MyoD1 in paraffin embedded Human rhabdomyosarcoma tissue, using ab133627 at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-258260-anti-myod1-antibody-epr6653-131-western-blot.jpg)
Western blot - Anti-MyoD1 antibody [EPR6653-131] (ab133627)All lanes : Anti-MyoD1 antibody [EPR6653-131] (ab133627) at 1/1000 dilution
Lane 1 : Rh30 (Human Rhabdomyosarcoma) Whole Cell Lysate at 5 µg
Lane 2 : Rh30 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab133627 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab133627 detects a band at 45 kDa, while this differs to its predicted molecular weight of 34 kDa, the banding pattern observed is consistent with what has been described in the literature PMID:19352326.
-
![Western blot - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-469821-antimyod1-antibody-epr6653131.jpg)
Western blot - Anti-MyoD1 antibody [EPR6653-131] (ab133627)All lanes : Anti-MyoD1 antibody [EPR6653-131] (ab133627) at 1/1000 dilution
Lane 1 : RD (Human muscle rhabdomyosarcoma) whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsBlocking and diluting buffer: 5% NFDM/TBST
Negative control: HEK-293, HeLa (PMID: 17028574 )
-
![Immunocytochemistry/ Immunofluorescence - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-469822-antimyod1-antibody-epr6653131-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MyoD1 antibody [EPR6653-131] (ab133627)Immunocytochemical analysis of 4% paraformaldehyde fixed, 0.1% TritonX-100 permeabilised RD cell line labeling MyoD1 with ab133627 at 1/100 diltuon. ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as a secondary antibody at 1/1000 dilution. Counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594). Nuclear staining: DAPI.
Confocal image showing nuclear staining in RD cell line
Negative control: HeLa (PMID: 17028574 ） -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-147517-anti-myod1-antibody-epr6653-131-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)ab133627 showing negative staining in Normal heart tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-147516-anti-myod1-antibody-epr6653-131-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)ab133627 showing negative staining in Thyroid gland carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-147515-anti-myod1-antibody-epr6653-131-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)ab133627 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-147514-anti-myod1-antibody-epr6653-131-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)ab133627 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-7-benefits-of-recombinant-antibodies.png)
Anti-MyoD1 antibody [EPR6653-131] (ab133627)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (17)
ab133627 has been referenced in 17 publications.
- Chen L et al. CPNE1 regulates myogenesis through the PERK-eIF2α pathway mediated by endoplasmic reticulum stress. Cell Tissue Res 391:545-560 (2023). PubMed: 36525128
- Feng J et al. TGF-β signaling and Creb5 cooperatively regulate Fgf18 to control pharyngeal muscle development. Elife 11:N/A (2022). PubMed: 36542062
- Hsu JY et al. SIX1 reprograms myogenic transcription factors to maintain the rhabdomyosarcoma undifferentiated state. Cell Rep 38:110323 (2022). PubMed: 35108532
- Jin Z et al. Role of skeletal muscle satellite cells in the repair of osteoporotic fractures mediated by β-catenin. J Cachexia Sarcopenia Muscle 13:1403-1417 (2022). PubMed: 35178895
- Sakai H et al. Uhrf1 governs the proliferation and differentiation of muscle satellite cells. iScience 25:103928 (2022). PubMed: 35243267