Name: Anti-MYOM1 antibody [EPR17322]
Brand: RabMAb
SKU: ab201228
Rating: 5 (1 reviews)

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Western blot - Anti-MYOM1 antibody [EPR17322] (ab201228)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17322] to MYOM1
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-MYOM1 antibody [EPR17322]
See all MYOM1 primary antibodies
Description

Rabbit monoclonal [EPR17322] to MYOM1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse heart and muscle lysates; Rat heart lysate; Human fetal heart lysate. IHC-P: Human, mouse and rat skeletal muscle tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17322
Isotype

IgG
Research areas

Alternative Versions
- Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab201228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/20000. Detects a band of approximately 188 kDa (predicted molecular weight: 188 kDa).
IHC-P		1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB 1/20000. Detects a band of approximately 188 kDa (predicted molecular weight: 188 kDa).
IHC-P 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent.
Sequence similarities

Contains 5 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Target information above from: UniProt accession P52179 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 8736 Human
- Entrez Gene: 17929 Mouse
- Entrez Gene: 316740 Rat
- Omim: 603508 Human
- SwissProt: P52179 Human
- SwissProt: Q62234 Mouse
- Unigene: 464469 Human
Alternative names
- 190 kDa connectin-associated protein antibody
- 190 kDa titin-associated protein antibody
- EH myomesin antibody
- MGC134946 antibody
- MGC134947 antibody
- MYOM1 antibody
- MYOM1_HUMAN antibody
- Myomesin (M protein) 1 (190kD) antibody
- Myomesin 1 (skelemin) 185kDa antibody
- Myomesin 1 185kDa antibody
- Myomesin 1, 185kDa antibody
- Myomesin family member 1 antibody
- Myomesin-1 antibody
- Skelemin antibody
see all

Western blot - Anti-MYOM1 antibody [EPR17322] (ab201228)

All lanes : Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse muscle lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa
Observed band size: 188 kDa

Exposure time: 1 minute

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-MYOM1 antibody [EPR17322] (ab201228)

All lanes : Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution

Lane 1 : Rat heart lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat kidney lysate
Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa
Observed band size: 188 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-MYOM1 antibody [EPR17322] (ab201228)

Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution + Human fetal heart tissue lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa
Observed band size: 188 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (ab201228)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (ab201228)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human tonsil tissue is a negative control for MYOM1. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (ab201228)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (ab201228)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-MYOM1 antibody [EPR17322] (ab201228)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab201228? Please let us know so that we can cite the reference in this datasheet.

ab201228 has been referenced in 2 publications.

Xie C et al. Analysis of characteristic genes and ceRNA regulation mechanism of endometriosis based on full transcriptional sequencing. Front Genet 13:902329 (2022). PubMed: 35938015
Chen S et al. MiR-135a is highly expressed and aggravates inflammatory response in sepsis by targeting MYOM1. Acta Biochim Pol 69:587-592 (2022). PubMed: 35977075

Customer reviews and Q&As

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Application

Western blot

Sample

Jaculus jaculus Tissue lysate - whole (muscle)

Gel Running Conditions

Reduced Denaturing (4-20%)

Loading amount

15 µg

Specification

muscle

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mai Tran

Verified customer

Submitted May 08 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (2)

Customer reviews and Q&As

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Western blot abreview for Anti-MYOM1 antibody [EPR17322]