Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)
![Immunoprecipitation - Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)](https://www.abcam.com/ps/products/16/ab16898/Images/ab16898-25782-anti-n-myc-antibody-ncm-ii-100-chip-grade-immunoprecipitation.jpg "Immunoprecipitation - Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)")
Key features and details
- Mouse monoclonal [NCM II 100] to n-Myc/MYCN
- Suitable for: IP, IHC-Fr, ICC/IF, WB, ChIP, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-n-Myc/MYCN antibody [NCM II 100]
See all n-Myc/MYCN primary antibodies -
Description
Mouse monoclonal [NCM II 100] to n-Myc/MYCN -
Host species
Mouse -
Specificity
This antibody reacts with N-myc/MYCN encoded proteins and their cleavage products.
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Tested applications
Suitable for: IP, IHC-Fr, ICC/IF, WB, ChIP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Fusion protein corresponding to Human n-Myc/MYCN. Recombinant fusion protein. Original clone reference: PubMed ID - 2426708.
Database link: P04198 -
Positive control
- IMR5 Cells
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General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.82% Sodium phosphate, 50% Glycerol -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
NCM II 100 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. |
|
| IHC-Fr |
Use at an assay dependent concentration.
See references. |
|
| ICC/IF |
Use at an assay dependent concentration.
Pubmed ID: 2426708. |
|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
See references. |
| ChIP |
Use at an assay dependent concentration. PubMed: 19495417
Suggested to be used at 2 ug. |
|
| Flow Cyt |
Use at an assay dependent concentration.
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IP
Use at an assay dependent concentration. It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. |
|
IHC-Fr
Use at an assay dependent concentration. See references. |
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ICC/IF
Use at an assay dependent concentration. Pubmed ID: 2426708. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa. See references. |
|
ChIP
Use at an assay dependent concentration. PubMed: 19495417 Suggested to be used at 2 ug. |
|
Flow Cyt
Use at an assay dependent concentration. It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
May function as a transcription factor. -
Involvement in disease
Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585]. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Developmental stage
Expressed during fetal development. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4613 Human
- Entrez Gene: 18109 Mouse
- Omim: 164840 Human
- SwissProt: P04198 Human
- SwissProt: P03966 Mouse
- Unigene: 25960 Human
- Unigene: 16469 Mouse
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Alternative names
- bHLHe37 antibody
- Class E basic helix-loop-helix protein 37 antibody
- MODED antibody
see all
Images
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Immunoprecipitation - Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)
Immunoprecipitation of N-Myc/MYCN from S35 methionine-labeled IMR-5 cells using ab16898 (lane 1) and Normal Mouse IgG (lane 2).
ab16898 used at 1 mg/sample.This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)](https://www.abcam.com/ps/products/16/ab16898/Images/ab16898-25781-anti-n-myc-antibody-ncm-ii-100-chip-grade-flow-cytometry.jpg)
Flow Cytometry - Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)Overlay histogram showing SH-SY5Y cells stained with ab16898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16898, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab16898 has been referenced in 39 publications.
- Yi JS et al. The synergy of BET inhibitors with aurora A kinase inhibitors in MYCN-amplified neuroblastoma is heightened with functional TP53. Neoplasia 23:624-633 (2021). PubMed: 34107377
- Santiago T et al. Multinational Retrospective Central Pathology Review of Neuroblastoma: Lessons Learned to Establish a Regional Pathology Referral Center in Resource-Limited Settings. Arch Pathol Lab Med 145:214-221 (2021). PubMed: 33501494
- Kilinc S et al. Oncogene-regulated release of extracellular vesicles. Dev Cell 56:1989-2006.e6 (2021). PubMed: 34118203
- Lundsten S et al. p53-Mediated Radiosensitization of 177Lu-DOTATATE in Neuroblastoma Tumor Spheroids. Biomolecules 11:N/A (2021). PubMed: 34827693
- Bian X et al. The MYC Paralog-PARP1 Axis as a Potential Therapeutic Target in MYC Paralog-Activated Small Cell Lung Cancer. Front Oncol 10:565820 (2020). PubMed: 33134168