Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)

What species is your lysate from?


For human samples, the n-myc oncoprotein has a predicted MW of 50 kDa (http://www.uniprot.org/uniprot/P04198), but runs in WB at around 64 kDa. Cleavage products would run at smaller MWs.

Thank you for your enquiry. According to the following publication, monoclonal ab16898 n-Myc antibody [clone NCM II 100] interacts with an epitope unique to an N-myc region not present in C-myc regions tested (3' 60% mouse C-myc coding region and 5' 30% C-myc coding region); please see the second paragraph on page 5930 of the publication. I hope this is helpful. Please do not hesitate to contact me if you require any further assistance.

Thank you for your enquiry. This antibody has been characterised with others raised against NMYC in the following paper which may be of help to you: Identification and characterisation of the NMYC gene product in human neuroblastoma cells by monoclonal antibodies with defined specificities. Ikegaki N et al. Prodedings of National Acadamy of Science USA. 83 p5929. This indiates that epitope for this antibody lies in the second exon of NMYC and it will recognise the cleavage products from NMYC. I hope this helps. Please get in touch again if you require any more advice. With thanks

Thank you for contacting us for technical support. The problem you are experiencing may be due to the fact that the antibody is very fragile. We recommend to aliquot and store at -20°C upon arrival. Can you tell me how you proceeded? If you can also tell me your order details I will look at the shipping details to investigate if the antibody suffered from a delay. I look forward to hearing from you to resolve this matter,

Thank you for your researcher's further information. The information that accompanies this antibody is that the buffer is comprised of no preservatives as detailed below: Preservative: None Constituents: 50% Glycerol, 0.05M Sodium phosphate. Furthermore we recommend that the antibody is stored at -20°C and that freeze / thaw cycles are avoided. Again as stated below: Storage instructions: Aliquot and store at -20°C. Avoid freeze / thaw cycles. We DO recommend that the antibody is diluted in PBS for certain applications prior to use. However, this certainly would NOT be recommended if the intention is to store the antibody in the fridge for two weeks, especially given the lack of preservative. It is therefore of no surprise to me that the antibody has developed mould ("contaminated"). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abcam Points which can be redeemed on a number of rewards (a further 100 points will be offered for an image).

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC-formalin fixed paraffin embedded sections. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). If you have any additional questions, please contact us again.