Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)
Key features and details
- Mouse monoclonal [17-3-4-1] to N6-methyladenosine (m6A)
- Suitable for: Northwestern, IP, Southern Blot
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-N6-methyladenosine (m6A) antibody [17-3-4-1]
See all N6-methyladenosine (m6A) primary antibodies -
Description
Mouse monoclonal [17-3-4-1] to N6-methyladenosine (m6A) -
Host species
Mouse -
Specificity
This antibody recognises N6-methyladenosine in both modified RNA and DNA. -
Tested applications
Suitable for: Northwestern, IP, Southern Blotmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to N6-methyladenosine (m6A) conjugated to bovine serum albumin. Hapten N6-methyladenosine-5'-mono-phosphate conjugated to BSA of all N6-methyladenosine
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Positive control
- methylated RNA (negative control non methylated RNA); single stranded DNA;
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
17-3-4-1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Northwestern |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
Best results are obtained by pre-binding the antibody to the beads before washing off unbound immunoglobulin. It is recommended to start with an excess of antibody in order to ensure the Protein G beads are saturated.Used 3µg in 500µl for IP which yielded positive results. |
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Southern Blot |
Use at an assay dependent concentration.
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Notes |
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Northwestern
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. Best results are obtained by pre-binding the antibody to the beads before washing off unbound immunoglobulin. It is recommended to start with an excess of antibody in order to ensure the Protein G beads are saturated.Used 3µg in 500µl for IP which yielded positive results. |
Southern Blot
Use at an assay dependent concentration. |
Target
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Relevance
N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. -
Alternative names
- m6A antibody
- N6-methyladenosine antibody
Images
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N6-methyladenosine (m6A) was immunoprecipitated using ab208577 from radiolabeled synthetic methylated transcript (Bound); radiolabeled Non methylated control (Non bound) and radiolabeled Input RNA control.
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Use of ab208577 for northwestern blot using synthetic methlyated and non methlyated RNA.
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Lane 1 Single stranded DNA
Lane 2 Double stranded DNA
Labelled with ab208577. Arrow shows m6A containing fragment.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab208577 has been referenced in 41 publications.
- Shu B et al. METTL3-mediated macrophage exosomal NEAT1 contributes to hepatic fibrosis progression through Sp1/TGF-ß1/Smad signaling pathway. Cell Death Discov 8:266 (2022). IP, IHC-P ; Rat . PubMed: 35585044
- Zhou L et al. Hypoxia-induced lncRNA STEAP3-AS1 activates Wnt/ß-catenin signaling to promote colorectal cancer progression by preventing m6A-mediated degradation of STEAP3 mRNA. Mol Cancer 21:168 (2022). WB ; Human . PubMed: 35986274
- Miao YQ et al. N(6)-adenosine-methyltransferase-14 promotes glioma tumorigenesis by repressing argininosuccinate synthase 1 expression in an m6A-dependent manner. Bioengineered 13:1858-1871 (2022). PubMed: 35012429
- Meng L et al. METTL14 suppresses pyroptosis and diabetic cardiomyopathy by downregulating TINCR lncRNA. Cell Death Dis 13:38 (2022). PubMed: 35013106
- Zheng H et al. m5 C and m6 A modification of long noncoding NKILA accelerates cholangiocarcinoma progression via the miR-582-3p-YAP1 axis. Liver Int 42:1144-1157 (2022). PubMed: 35274813