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Anti-Nanog antibody (ab80892)

Reviews (18)Q&A (9)References (335)

Key features and details

  • Rabbit polyclonal to Nanog
  • Suitable for: ICC, ICC/IF, IHC-P, Flow Cyt, IHC - Wholemount, WB
  • Reacts with: Mouse
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Nanog antibody [EPR2027(2)] (ab109250)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Nanog antibody
    See all Nanog primary antibodies

  • Description

    Rabbit polyclonal to Nanog

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, ICC/IF, IHC-P, Flow Cyt, IHC - Wholemount, WBmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab80892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC (1)
Use at an assay dependent concentration.
ICC/IF (5)
Use at an assay dependent concentration.
IHC-P (5)
Use at an assay dependent concentration.
Flow Cyt (2)
Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC - Wholemount (1)
Use at an assay dependent concentration.
WB (3)
Use at an assay dependent concentration.
Notes
ICC
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC - Wholemount
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

Target

  • Function

    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

  • Tissue specificity

    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

  • Sequence similarities

    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • Developmental stage

    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Embryonic stem cell specific homeobox protein (Nanog) antibody
      • ENK antibody
      • FLJ12581 antibody
      • hNanog antibody
      • Homeobox protein NANOG antibody
      • Homeobox transcription factor Nanog antibody
      • homeobox transcription factor Nanog-delta 48 antibody
      • NANOG antibody
      • Nanog homeobox antibody
      • NANOG_HUMAN antibody
      see all

    References (335)

    Publishing research using ab80892? Please let us know so that we can cite the reference in this datasheet.

    ab80892 has been referenced in 335 publications.

    • Lee J  et al. Production of large, defined genome modifications in rats by targeting rat embryonic stem cells. Stem Cell Reports 18:394-409 (2023). PubMed: 36525967
    • Morera C  et al. CRISPR-mediated correction of skeletal muscle Ca2+ handling in a novel DMD patient-derived pluripotent stem cell model. Neuromuscul Disord 32:908-922 (2022). PubMed: 36418198
    • Botigelli RC  et al. Acquisition and maintenance of pluripotency are influenced by fibroblast growth factor, leukemia inhibitory factor, and 2i in bovine-induced pluripotent stem cells. Front Cell Dev Biol 10:938709 (2022). PubMed: 36187479
    • Song JH  et al. Developmental Potency and Metabolic Traits of Extended Pluripotency Are Faithfully Transferred to Somatic Cells via Cell Fusion-Induced Reprogramming. Cells 11:N/A (2022). PubMed: 36291134
    • Yan S  et al. CTGF Promotes the Osteoblast Differentiation of Human Periodontal Ligament Stem Cells by Positively Regulating BMP2/Smad Signal Transduction. Biomed Res Int 2022:2938015 (2022). PubMed: 36158888
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry abreview for Anti-Nanog antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Rabbit Cultured Cells (brain)
    Permeabilization
    Yes - tritonx100
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Kyungjoo Seong

    Verified customer

    Submitted Oct 31 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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