Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-NAT10 antibody [EPR18663] (ab194297)

Reviews (1) Submit a question References (11)
Immunocytochemistry/ Immunofluorescence - Anti-NAT10 antibody [EPR18663] (ab194297)
  • Western blot - Anti-NAT10 antibody [EPR18663] (ab194297)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)
  • Flow Cytometry (Intracellular) - Anti-NAT10 antibody [EPR18663] (ab194297)
  • Western blot - Anti-NAT10 antibody [EPR18663] (ab194297)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)
  • Immunocytochemistry/ Immunofluorescence - Anti-NAT10 antibody [EPR18663] (ab194297)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)
  • Immunoprecipitation - Anti-NAT10 antibody [EPR18663] (ab194297)
  • Anti-NAT10 antibody [EPR18663] (ab194297)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18663] to NAT10
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-NAT10 antibody [EPR18663]
    See all NAT10 primary antibodies

  • Description

    Rabbit monoclonal [EPR18663] to NAT10

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain and fetal heart lysates; Mouse brain, rat heart and rat spleen lysates. IHC-P: Human colon, mouse stomach and rat colon tissues. ICC/IF: HeLa and NIH/3T3 cells. IP: HeLa cell lysate. Flow Cyt (intra): NIH/3T3 cell lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/600.
WB
1/2000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
ICC/IF (1)
1/2000.
IP
1/80.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/600.
WB
1/2000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
ICC/IF
1/2000.
IP
1/80.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division.

  • Sequence similarities

    Belongs to the UPF0202 family.
    Contains 1 N-acetyltransferase domain.

  • Cellular localization

    Nucleus > nucleolus. Nucleolar in interphase and redistributes to the perichromosomal layer and to the midbody during telophase.
  • Information by UniProt

  • Database links

    • Alternative names

      • ac4C enzyme antibody
      • ac4C writer antibody
      • DKFZp434C116 antibody
      • FLJ10774 antibody
      • FLJ12179 antibody
      • FLJ23850 antibody
      • hALP antibody
      • KIAA1709 antibody
      • N acetyltransferase 10 antibody
      • N acetyltransferase 10 GCN5 related antibody
      • N acetyltransferase like antibody
      • N acetyltransferase like protein antibody
      • N-acetyltransferase 10 antibody
      • NAT10 antibody
      • NAT10_HUMAN antibody
      • NET43 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-NAT10 antibody [EPR18663] (ab194297)
      Immunocytochemistry/ Immunofluorescence - Anti-NAT10 antibody [EPR18663] (ab194297)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NAT10 with ab194297 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing nuclear staining on HeLa cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:-
      -ve control 1: ab194297 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

       

    • Western blot - Anti-NAT10 antibody [EPR18663] (ab194297)
      Western blot - Anti-NAT10 antibody [EPR18663] (ab194297)
      All lanes : Anti-NAT10 antibody [EPR18663] (ab194297) at 1/2000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat heart lysate
      Lane 3 : Rat spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 116 kDa
      Observed band size: 116 kDa


      Exposure time: 30 seconds
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)

      Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on rat colon tissue is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-NAT10 antibody [EPR18663] (ab194297)
      Flow Cytometry (Intracellular) - Anti-NAT10 antibody [EPR18663] (ab194297)

      Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryo) cells labelling NAT10 (red) with purified ab194297 at dilution of 1/600. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody. 

       

       

    • Western blot - Anti-NAT10 antibody [EPR18663] (ab194297)
      Western blot - Anti-NAT10 antibody [EPR18663] (ab194297)
      All lanes : Anti-NAT10 antibody [EPR18663] (ab194297) at 1/2000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 116 kDa
      Observed band size: 116 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on Human colon tissue is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-NAT10 antibody [EPR18663] (ab194297)
      Immunocytochemistry/ Immunofluorescence - Anti-NAT10 antibody [EPR18663] (ab194297)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling NAT10 with ab194297 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing nuclear staining on NIH/3T3 cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:-
      -ve control 1: ab194297 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)

      Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on mouse stomach tissue is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-NAT10 antibody [EPR18663] (ab194297)
      Immunoprecipitation - Anti-NAT10 antibody [EPR18663] (ab194297)

      NAT10 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate with ab194297 at 1/80 dilution.

      Lane 1: HeLa cell lysate 10ug (Input).

      Lane 2: ab194297 IP in HeLa cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194297 in HeLa cell lysate.

      Western blot was performed from the immunoprecipitate using ab194297 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

    • Anti-NAT10 antibody [EPR18663] (ab194297)
      Anti-NAT10 antibody [EPR18663] (ab194297)

    References (11)

    Publishing research using ab194297? Please let us know so that we can cite the reference in this datasheet.

    ab194297 has been referenced in 11 publications.

    • Qiu T  et al. GATA4 regulates osteogenic differentiation by targeting miR-144-3p. Exp Ther Med 23:83 (2022). PubMed: 34934452
    • Feng Z  et al. The LINC00623/NAT10 signaling axis promotes pancreatic cancer progression by remodeling ac4C modification of mRNA. J Hematol Oncol 15:112 (2022). PubMed: 35978332
    • Fei X  et al. Amniotic fluid mesenchymal stem cells repair mouse corneal cold injury by promoting mRNA N4-acetylcytidine modification and ETV4/JUN/CCND2 signal axis activation. Hum Cell 34:86-98 (2021). PubMed: 33010000
    • Zhu Z  et al. NAT10 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Mediating N4-Acetylcytidine Modification of Gremlin 1. Stem Cells Int 2021:8833527 (2021). PubMed: 33953754
    • Tao W  et al. NAT10 as a potential prognostic biomarker and therapeutic target for HNSCC. Cancer Cell Int 21:413 (2021). PubMed: 34362389
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-NAT10 antibody [EPR18663]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (PC12, a cell line derived from a pheochromocytoma)
    Permeabilization
    Yes - 0.1% TritonX-100
    Specification
    PC12, a cell line derived from a pheochromocytoma
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 18 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com