Recombinant Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)
![Western blot - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)](https://www.abcam.com/ps/products/225/ab225844/Images/ab225844-314786-anti-natriuretic-peptides-a-antibody-epr22089-283-western-blot.jpg "Western blot - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22089-283] to Natriuretic peptides A
- Suitable for: IHC-Fr, IHC-P, IP, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Natriuretic peptides A antibody [EPR22089-283]
See all Natriuretic peptides A primary antibodies -
Description
Rabbit monoclonal [EPR22089-283] to Natriuretic peptides A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat heart tissue lysate. IHC-P: Mouse heart and heart atrium tissues; Rat heart atrium tissue. IHC-Fr: Mouse and rat heart tissues. IP: Mouse heart tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22089-283 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/100.
Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| IP |
1/30.
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| WB |
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
|
| Notes |
|---|
|
IHC-Fr
1/100. Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
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WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa). |
Target
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Function
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Involvement in disease
Atrial standstill 2
Atrial fibrillation, familial, 6 -
Sequence similarities
Belongs to the natriuretic peptide family. -
Post-translational
modificationsCleaved by CORIN upon secretion to produce the functional hormone.
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 230899 Mouse
- Entrez Gene: 24602 Rat
- SwissProt: P05125 Mouse
- SwissProt: P01161 Rat
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Alternative names
- ANF antibody
- ANF_HUMAN antibody
- ANP antibody
see all
Images
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Western blot - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)All lanes : Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 16 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Exposure time : Lane 1: 3 minutes; Lane 2: 8 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 16291870).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)](https://www.abcam.com/ps/products/225/ab225844/Images/ab225844-314787-anti-natriuretic-peptides-a-antibody-epr22089-283-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strong cytoplasmic staining mainly in the atria and at lower levels in ventricle of mouse heart (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)](https://www.abcam.com/ps/products/225/ab225844/Images/ab225844-314790-anti-natriuretic-peptides-a-antibody-epr22089-283-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart tissue labeling Natriuretic peptides A with ab225844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in cardiac muscle of mouse atrium is observed.
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)](https://www.abcam.com/ps/products/225/ab225844/Images/ab225844-314791-anti-natriuretic-peptides-a-antibody-epr22089-283-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with ab225844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in cardiac muscle of rat atrium is observed.
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)](https://www.abcam.com/ps/products/225/ab225844/Images/ab225844-314788-anti-natriuretic-peptides-a-antibody-epr22089-283-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)Immunohistochemical analysis of paraffin-embedded mouse heart atrium tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in cardiac muscle of mouse atrium (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)](https://www.abcam.com/ps/products/225/ab225844/Images/ab225844-314792-anti-natriuretic-peptides-a-antibody-epr22089-283-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)Natriuretic peptides A was immunoprecipitated from 0.35 mg Mouse heart tissue lysate with ab225844 at 1/1000 dilution. Western blot was perfromed from the immunoprecipitate using ab225844 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Lane 1: Mouse heart lysate 10 μg (Input).
Lane 2: ab225844 IP in Mouse heart tissue lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225844 in Mouse heart lysate (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)](https://www.abcam.com/ps/products/225/ab225844/Images/ab225844-314789-anti-natriuretic-peptides-a-antibody-epr22089-283-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)Immunohistochemical analysis of paraffin-embedded rat heart atrium tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in cardiac muscle of rat atrium (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)](https://www.abcam.com/ps/products/225/ab225844/Images/ab225844-8-benefits-of-recombinant-antibodies.png)
Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (9)
ab225844 has been referenced in 9 publications.
- Jiang X et al. MCC950 ameliorates ventricular arrhythmia vulnerability induced by heart failure. Bioengineered 13:8593-8604 (2022). PubMed: 35287557
- Gong H et al. Obstructive Sleep Apnea Impacts Cardiac Function in Dilated Cardiomyopathy Patients Through Circulating Exosomes. Front Cardiovasc Med 9:699764 (2022). PubMed: 35321104
- Li Z et al. Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting Proteostasis. Int J Biol Sci 18:2018-2031 (2022). PubMed: 35342336
- Yang Y et al. The lncRNA MIAT regulates CPT-1a mediated cardiac hypertrophy through m6A RNA methylation reading protein Ythdf2. Cell Death Discov 8:167 (2022). PubMed: 35383152
- Liang Q et al. Postnatal Deletion of Bmal1 in Cardiomyocyte Promotes Pressure Overload Induced Cardiac Remodeling in Mice. J Am Heart Assoc 11:e025021 (2022). PubMed: 35730615
- Huo S et al. Alleviation of Inflammation and Oxidative Stress in Pressure Overload-Induced Cardiac Remodeling and Heart Failure via IL-6/STAT3 Inhibition by Raloxifene. Oxid Med Cell Longev 2021:6699054 (2021). PubMed: 33824698
- Xie Y et al. The proteasome activator REG? accelerates cardiac hypertrophy by declining PP2Aca-SOD2 pathway. Cell Death Differ N/A:N/A (2020). PubMed: 32424140
- Cheng Z et al. lncRNA ZEB2-AS1 stimulates cardiac hypertrophy by downregulating PTEN. Exp Ther Med 20:92 (2020). PubMed: 32973941
- Ai F et al. Schisandrin B attenuates pressure overload-induced cardiac remodeling in mice by inhibiting the MAPK signaling pathway. Exp Ther Med 18:4645-4652 (2019). PubMed: 31798701