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Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)

Reviews (1)Q&A (2)References (7)
Immunocytochemistry/ Immunofluorescence - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
  • Flow Cytometry - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
  • Western blot - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)

Key features and details

  • Mouse monoclonal [N134/12] to Nav1.8/SCN10A
  • Suitable for: ICC, ICC/IF, IHC-Fr, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human, Monkey
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Nav1.8/SCN10A antibody [N134/12]
    See all Nav1.8/SCN10A primary antibodies

  • Description

    Mouse monoclonal [N134/12] to Nav1.8/SCN10A

  • Host species

    Mouse

  • Specificity

    No cross reactivity against other Nav channels.

  • Tested applications

    Suitable for: ICC, ICC/IF, IHC-Fr, WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey

  • Immunogen

    Recombinant fragment within Rat Nav1.8/SCN10A aa 1700-2000. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q62968

  • Positive control

    • WB: COS cell lysate transiently expressing Nav1.8/SCN10A. Flow Cyt: SH-SY5Y cells. IHC-P: Mouse backskin tissue. ICC: HaCaT cells

  • General notes

    The clone number has been updated from S134-12 to N134/12, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab93616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 220 kDa.
IHC-P (1)
Use a concentration of 0.1 - 1 µg/ml.
Flow Cyt
Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Notes
ICC
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 220 kDa.
IHC-P
Use a concentration of 0.1 - 1 µg/ml.
Flow Cyt
Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.

  • Tissue specificity

    Expressed in the dorsal root ganglia and sciatic nerve.

  • Involvement in disease

    Episodic pain syndrome, familial, 2

  • Sequence similarities

    Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
    Contains 1 IQ domain.

  • Domain

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

  • Post-translational
    modifications

    Ubiquitinated by NEDD4L; which promotes its endocytosis.
    Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.

  • Cellular localization

    Cell membrane. It can be translocated to the cell membrane through association with S100A10.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • hPN3 antibody
    • mPN3 antibody
    • Peripheral nerve sodium channel 3 antibody
    • Pn3 (gene name) antibody
    • PN3 antibody
    • SCN10A antibody
    • SCNAA_HUMAN antibody
    • Sensory neuron sodium channel antibody
    • Sns (gene name) antibody
    • SNS antibody
    • Sodium channel protein type 10 subunit alpha antibody
    • Sodium channel protein type X alpha subunit antibody
    • Sodium channel protein type X subunit alpha antibody
    • Voltage-gated sodium channel alpha subunit Nav1.8 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.8 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
    Immunocytochemistry/ Immunofluorescence - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)

    Immunofluorescent analysis of 100% methanol-fixed (10 minutes at -20°C) HaCaT (human keratinocytes) cells labelling Nav1.8/SCN10A with ab93616 at 1/100 dilution, followed by Goat Anti-Mouse (FITC) secondary antibody at 1/50 dilution (Green). Fluorescence image showing cytoplasmic staining and some dull nuclear staining on HaCaT cell line.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)

    Paraffin-embedded mouse backskin tissue stained for Nav1.8/SCN10A using ab93616 at 1/100 dilution in immunohistochemical analysis.

    Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 dilution for 1 hour at room temperature.

  • Flow Cytometry - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
    Flow Cytometry - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)

    Overlay histogram showing SH-SY5Y cells stained with ab93616 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93616, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
    Western blot - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
    Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616) at 1/1000 dilution + Monkey COS cell lysate transiently expressing Nav1.8/SCN10A. at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP

    Predicted band size: 220 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (7)

Publishing research using ab93616? Please let us know so that we can cite the reference in this datasheet.

ab93616 has been referenced in 7 publications.

  • Matsuo S  et al. Central, but not peripheral, nervous system ERK2 is essential for itch signals in murine allergic skin inflammation. Allergy 76:3422-3432 (2021). PubMed: 33884632
  • Atmaramani RR  et al. Conserved Expression of Nav1.7 and Nav1.8 Contribute to the Spontaneous and Thermally Evoked Excitability in IL-6 and NGF-Sensitized Adult Dorsal Root Ganglion Neurons In Vitro. Bioengineering (Basel) 7:N/A (2020). PubMed: 32429423
  • Ni S  et al. Sensory innervation in porous endplates by Netrin-1 from osteoclasts mediates PGE2-induced spinal hypersensitivity in mice. Nat Commun 10:5643 (2019). PubMed: 31822662
  • Goldstein RH  et al. Location and Plasticity of the Sodium Spike Initiation Zone in Nociceptive Terminals In Vivo. Neuron 102:801-812.e5 (2019). PubMed: 30926280
  • Cejudo-Roman A  et al. The voltage-gated sodium channel nav1.8 is expressed in human sperm. PLoS One 8:e76084 (2013). Flow Cyt, ICC/IF ; Human . PubMed: 24086692
  • Ramachandra R  et al. Identification of CaV channel types expressed in muscle afferent neurons. J Neurophysiol 110:1535-43 (2013). PubMed: 23843437
  • Ramachandra R  et al. Tetrodotoxin-resistant voltage-dependent sodium channels in identified muscle afferent neurons. J Neurophysiol 108:2230-41 (2012). IHC . PubMed: 22855776

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nav1.8 antibody [S134-12]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako FLEX Peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako High pH
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 02 2014

Question

How was non-crossreactivity with other Nav channels of both of these antibodies determined?

Read More
Answer

The specificity of these antibodies to their target Nav channel was determined by performing western blot analysis on lysates from various HEK cell lines expressing one Nav channel. These antibodies were used to detect the presence of the channel in the lysates, and it was determined that ab85015 only detects Nav1.7 and ab93616 only detects Nav1.8.
Both these products were tested in the neuroblastoma cell line SH-SY5Y due to expression in dorsal root ganglion. These products are both tested and guaranteed to be specific for their respective Nav channels in the applications and species listed on their datasheets.

Read More

Question

Phone call requesting information of how the anti-Nav1.8 antibodies (ab66743 and ab93616) have been used in staining paraffin embedded tissue sections.

Read More
Answer

Thank you for contacting us in regards to the anti-Nav1.8 antibodies ab66743, ab83936, ab93616. I am sorry for the delay in my reply, I have been seeking information in regards to the IHC protocols used with these antibodies.

The antibodies you were most interested in I believe were ab66743 and ab93616 which are both directed towards the C-terminal portion of Nav1.8. I have unfortunately found that ab66743 has not been tested in IHC, only ICC of Spermatozoa. This information information is now being updated. I am sorry that this was not made clear previously.

With the antibody ab93616, the antibody was used to stain mouse skin tissue. This was performed on PFA fixed paraffin embedded tissue. Citrate buffer (pH 6) was used for antigen retrieval using a microwave to heat on high power for 2x5minutes. The primary antibody was incubated with the slide at room temperature for 1 houra dilution of1 ug/mLin PBST. The secondary antibody was incubated for1 hour at room temperature. The results obtained can be observed in the attachment to this email.

The ab83936, raised against the N-terminal portion of Nav1.8 has also been used in IHC. Citrate buffer was again used for the antigen retrieval (pH6) using the microwave for 10 minutesThe antibody was used in a dilution of 1/300 at room temperature for 1 hour.

I would like to reassure you that both ab93616 and ab83936 would be covered by the Abrpomise and if you were to have any problems in obtaining the expected staining and it has been used as according to the datasheet you would be entitled to a replacement antibody of your choice, credit note or refund. More information on the Abpromise can be found from the following link:

https://www.abcam.com/abpromise

Alternatively, if you would like to try ab83936 in IHC I would be able to offer you a testing discount. As we have not tested this antibody ourselves in IHC, if you were to purchase this antibody (normal price), test in in IHC and share the results with us through an Abreview, you would be entitled to a free primary antibody (or the equivalent off any product in our catalogue). More information on this scheme can be found from the following link:

https://www.abcam.com/collaboratordiscount

The only constraint to this offer is that the antibody must be purchased, tested, the Abreview submittedand the free product claimed within a 4 month period. If you would liketo participate in this schemeplease do letme know as adiscount code needs to be issuedprior to purchase of the antibody to betested.

Unfortunately I can confirm that Imperial do not get a discount from Abcam but I would be able to give you an initial discount of 5% if you would be interested in purchasing any of these antibodies.

I hope this information has been of help.If you would like any further information please do not hesitate to contact us again.

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