Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
![Immunocytochemistry/ Immunofluorescence - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)](https://www.abcam.com/ps/products/93/ab93616/Images/ab93616-479365-anti-Nav18-SCN10A-antibody-N13412-ICC-human-HaCaT-cells.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)")
Key features and details
- Mouse monoclonal [N134/12] to Nav1.8/SCN10A
- Suitable for: ICC, ICC/IF, IHC-Fr, WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human, Monkey
- Isotype: IgG2a
Overview
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Product name
Anti-Nav1.8/SCN10A antibody [N134/12]
See all Nav1.8/SCN10A primary antibodies -
Description
Mouse monoclonal [N134/12] to Nav1.8/SCN10A -
Host species
Mouse -
Specificity
No cross reactivity against other Nav channels. -
Tested applications
Suitable for: ICC, ICC/IF, IHC-Fr, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Monkey -
Immunogen
Recombinant fragment within Rat Nav1.8/SCN10A aa 1700-2000. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q62968 -
Positive control
- WB: COS cell lysate transiently expressing Nav1.8/SCN10A. Flow Cyt: SH-SY5Y cells. IHC-P: Mouse backskin tissue. ICC: HaCaT cells
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General notes
The clone number has been updated from S134-12 to N134/12, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N134/12 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 220 kDa.
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| IHC-P | (1) |
Use a concentration of 0.1 - 1 µg/ml.
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| Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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ICC
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 220 kDa. |
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IHC-P
Use a concentration of 0.1 - 1 µg/ml. |
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Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms. -
Tissue specificity
Expressed in the dorsal root ganglia and sciatic nerve. -
Involvement in disease
Episodic pain syndrome, familial, 2 -
Sequence similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
Contains 1 IQ domain. -
Domain
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsUbiquitinated by NEDD4L; which promotes its endocytosis.
Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents. -
Cellular localization
Cell membrane. It can be translocated to the cell membrane through association with S100A10. - Information by UniProt
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Database links
- Entrez Gene: 6336 Human
- Entrez Gene: 20264 Mouse
- Entrez Gene: 29571 Rat
- Omim: 604427 Human
- SwissProt: Q9Y5Y9 Human
- SwissProt: P70276 Mouse
- SwissProt: Q6QIY3 Mouse
- SwissProt: Q62968 Rat
see all -
Alternative names
- hPN3 antibody
- mPN3 antibody
- Peripheral nerve sodium channel 3 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
Immunofluorescent analysis of 100% methanol-fixed (10 minutes at -20°C) HaCaT (human keratinocytes) cells labelling Nav1.8/SCN10A with ab93616 at 1/100 dilution, followed by Goat Anti-Mouse (FITC) secondary antibody at 1/50 dilution (Green). Fluorescence image showing cytoplasmic staining and some dull nuclear staining on HaCaT cell line.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)](https://www.abcam.com/ps/products/93/ab93616/Images/ab93616-309702-anti-nav18-antibody-n134-12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)Paraffin-embedded mouse backskin tissue stained for Nav1.8/SCN10A using ab93616 at 1/100 dilution in immunohistochemical analysis.
Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 dilution for 1 hour at room temperature.
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![Flow Cytometry - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)](https://www.abcam.com/ps/products/93/ab93616/Images/ab93616-108653-anti-nav18-antibody-n134-12-flow-cytometry.jpg)
Flow Cytometry - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)Overlay histogram showing SH-SY5Y cells stained with ab93616 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93616, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Western blot - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)](https://www.abcam.com/ps/products/93/ab93616/Images/ab93616-309703-anti-nav18-antibody-n134-12-western-blot.jpg)
Western blot - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616) at 1/1000 dilution + Monkey COS cell lysate transiently expressing Nav1.8/SCN10A. at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP
Predicted band size: 220 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab93616 has been referenced in 7 publications.
- Matsuo S et al. Central, but not peripheral, nervous system ERK2 is essential for itch signals in murine allergic skin inflammation. Allergy 76:3422-3432 (2021). PubMed: 33884632
- Atmaramani RR et al. Conserved Expression of Nav1.7 and Nav1.8 Contribute to the Spontaneous and Thermally Evoked Excitability in IL-6 and NGF-Sensitized Adult Dorsal Root Ganglion Neurons In Vitro. Bioengineering (Basel) 7:N/A (2020). PubMed: 32429423
- Ni S et al. Sensory innervation in porous endplates by Netrin-1 from osteoclasts mediates PGE2-induced spinal hypersensitivity in mice. Nat Commun 10:5643 (2019). PubMed: 31822662
- Goldstein RH et al. Location and Plasticity of the Sodium Spike Initiation Zone in Nociceptive Terminals In Vivo. Neuron 102:801-812.e5 (2019). PubMed: 30926280
- Cejudo-Roman A et al. The voltage-gated sodium channel nav1.8 is expressed in human sperm. PLoS One 8:e76084 (2013). Flow Cyt, ICC/IF ; Human . PubMed: 24086692
- Ramachandra R et al. Identification of CaV channel types expressed in muscle afferent neurons. J Neurophysiol 110:1535-43 (2013). PubMed: 23843437
- Ramachandra R et al. Tetrodotoxin-resistant voltage-dependent sodium channels in identified muscle afferent neurons. J Neurophysiol 108:2230-41 (2012). IHC . PubMed: 22855776