Name: Anti-NCAPH2 antibody [EPR17170]
Brand: RabMAb
SKU: ab200659
Rating: 4 (1 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-NCAPH2 antibody [EPR17170] (ab200659)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17170] to NCAPH2
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-NCAPH2 antibody [EPR17170]
See all NCAPH2 primary antibodies
Description

Rabbit monoclonal [EPR17170] to NCAPH2
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HepG2, Jurkat, RAW 264.7 and PC-12 whole cell lysates; Mouse and rat heart lysates. IHC-P: Human tonsil, Human clear cell carcinoma of kidney, Human hepatocellular carcinoma and rat kidney tissues. ICC/IF: HeLa and Jurkat cells. IP: HeLa whole cell lysate. Flow: Jurkat (human acute T cell leukemia)
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17170
Isotype

IgG
Research areas

Alternative Versions
- Anti-NCAPH2 antibody [EPR17170] - BSA and Azide free (ab251316)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab200659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		Use a concentration of 10 µg/ml.
WB		1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P		1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF	(1)	1/100.
IP		1/60.

Notes
Flow Cyt (Intra) Use a concentration of 10 µg/ml.
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
IP 1/60.

Function

Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development.
Sequence similarities

Belongs to the CND2 H2 (condensin-2 subunit 2) family.
Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization

Nucleus. Chromosome. Distributed along the arms of chromosomes assembled in vivo and in vitro.
Target information above from: UniProt accession Q6IBW4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 29781 Human
- Entrez Gene: 52683 Mouse
- Entrez Gene: 300149 Rat
- Omim: 611230 Human
- SwissProt: Q6IBW4 Human
- SwissProt: Q8BSP2 Mouse
- SwissProt: Q4V8I2 Rat
- Unigene: 180903 Human
- Unigene: 143167 Mouse
- Unigene: 4227 Rat
see all
Alternative names
- CAP H2 antibody
- CAP H2 subunit of the condensin II complex antibody
- CAPH2 antibody
- Chromosome associated protein H2 antibody
- Chromosome-associated protein H2 antibody
- CNDH2_HUMAN antibody
- Condensin-2 complex subunit H2 antibody
- hCAP H2 antibody
- hCAP-H2 antibody
- Kleisin beta antibody
- Kleisin-beta antibody
- Ncaph2 antibody
- Non SMC condensin II complex subunit H2 antibody
- Non-SMC condensin II complex subunit H2 antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-NCAPH2 antibody [EPR17170] (ab200659)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NCAPH2 with ab200659 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Nuclear and weakly cytoplasm staining on HeLa cell line is observed.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab200659 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-NCAPH2 antibody [EPR17170] (ab200659)

All lanes : Anti-NCAPH2 antibody [EPR17170] (ab200659) at 1/10000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry (Intracellular) - Anti-NCAPH2 antibody [EPR17170] (ab200659)

ab200659 staining NCAPH2 in Jurkat (human acute T cell leukemia) cellsby intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/240. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Western blot - Anti-NCAPH2 antibody [EPR17170] (ab200659)

All lanes : Anti-NCAPH2 antibody [EPR17170] (ab200659) at 1/1000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-NCAPH2 antibody [EPR17170] (ab200659)

All lanes : Anti-NCAPH2 antibody [EPR17170] (ab200659) at 1/1000 dilution

Lane 1 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPH2 antibody [EPR17170] (ab200659)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NCAPH2 with ab200659 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear and weakly cytoplasm staining on Human tonsil tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPH2 antibody [EPR17170] (ab200659)

Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling NCAPH2 with ab200659 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear and cytoplasm staining on Human clear cell carcinoma of kidney tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPH2 antibody [EPR17170] (ab200659)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling NCAPH2 with ab200659 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear and weakly cytoplasmic staining on Human hepatocellular carcinoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAPH2 antibody [EPR17170] (ab200659)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling NCAPH2 with ab200659 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear and weakly cytoplasmic staining on rat kidney is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NCAPH2 antibody [EPR17170] (ab200659)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NCAPH2 with ab200659 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Nuclear and weakly cytoplasm staining on Jurkat cell line is observed.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab200659 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunoprecipitation - Anti-NCAPH2 antibody [EPR17170] (ab200659)

NCAPH2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200659 at 1/60 dilution.
Western blot was performed from the immunoprecipitate using ab200659 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab200659 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200659 in HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-NCAPH2 antibody [EPR17170] (ab200659)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab200659? Please let us know so that we can cite the reference in this datasheet.

ab200659 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Permeabilization

Yes - 0.5% Triton X-100

Specification

HeLa

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

Submitted Jan 04 2018

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-NCAPH2 antibody [EPR17170]

Post-translational
modifications