Anti-NDUFB4 antibody [17G3D9E12] (ab110243)
![Western blot - Anti-NDUFB4 antibody [17G3D9E12] (ab110243)](https://www.abcam.com/ps/products/110/ab110243/Images/ab110243-123422-anti-ndufb4-antibody-17g3d9e12-western-blot.jpg "Western blot - Anti-NDUFB4 antibody [17G3D9E12] (ab110243)")
Key features and details
- Mouse monoclonal [17G3D9E12] to NDUFB4
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-NDUFB4 antibody [17G3D9E12]
See all NDUFB4 primary antibodies -
Description
Mouse monoclonal [17G3D9E12] to NDUFB4 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Cow, Human -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Isolated mitochondria from Human heart. HeLa cels. Cultured Human fibroblasts.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Near homogeneity as judged by SDS-PAGE (>95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
17G3D9E12 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol. |
| WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa.
|
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| ICC/IF |
Use a concentration of 5 - 15 µg/ml.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol. |
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
(heat-induced antigen-retrieval improves signal). ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol. |
|
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa. |
|
ICC/IF
Use a concentration of 5 - 15 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol. |
|
Flow Cyt
Use a concentration of 1 µg/ml. (heat-induced antigen-retrieval improves signal). ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Sequence similarities
Belongs to the complex I NDUFB4 subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4710 Human
- Entrez Gene: 288088 Rat
- Omim: 603840 Human
- SwissProt: O95168 Human
- Unigene: 304613 Human
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Alternative names
- B15 antibody
- CI-B15 antibody
- Complex I B15 subunit antibody
see all
Images
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Western blot - Anti-NDUFB4 antibody [17G3D9E12] (ab110243)All lanes : Anti-NDUFB4 antibody [17G3D9E12] (ab110243) at 0.5 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from cow heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Predicted band size: 15 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [17G3D9E12] (ab110243)](https://www.abcam.com/ps/products/110/ab110243/Images/ab110243-123421-anti-ndufb4-antibody-17g3d9e12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [17G3D9E12] (ab110243)Mitochondrial localization of complex I visualized by immunocytochemistry using aab110243.
Cultured Human fibroblasts were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110243 at 15µg/ml, followed by a fluorescent goat-anti-mouse IgG. -
![Flow Cytometry - Anti-NDUFB4 antibody [17G3D9E12] (ab110243)](https://www.abcam.com/ps/products/110/ab110243/Images/ab110243-123420-anti-ndufb4-antibody-17g3d9e12-flow-cytometry.jpg)
Flow Cytometry - Anti-NDUFB4 antibody [17G3D9E12] (ab110243)HeLa cells were stained with 1 µg/mL ab110243 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [17G3D9E12] (ab110243)](https://www.abcam.com/ps/products/110/ab110243/Images/ab110243-123419-anti-ndufb4-antibody-17g3d9e12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [17G3D9E12] (ab110243)This image is courtesy of Philippa Hepplewhite, University of Newcastle, UKImmunohistochemistical detection of Anti-NDUFB4 antibody [17G3D9E12] (ab110243; 1/100, incubated for 1hr) on formaldehyde fixed human cerebellum sections. Antigen retrieval step: Heat mediated. Permeabilization: No. ab110243 was used to investigate respiratory deficiency in 5µm cut sections of FFPE human cerebellum. Sections were cut and mounted on to glass adhesion slides for staining. The antibody stained specifically, identifying both normal neurons as well as those exhibiting respiratory deficiency. Non-specific background staining was not evident, and positive staining was crisp and punctate. Detection - Menarini Diagnostics X-Cell Plus HRP-Polymer Kit. SigmaFast DAB tablets were used for the final stage of staining.
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab110243 has been referenced in 15 publications.
- Anand R et al. MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes. Life Sci Alliance 3:N/A (2020). PubMed: 32788226
- Xin JW et al. Signature of high altitude adaptation in the gluteus proteome of the yak. J Exp Zool B Mol Dev Evol 334:362-372 (2020). PubMed: 32779369
- Liang JR et al. A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation. Cell 180:1160-1177.e20 (2020). PubMed: 32160526
- Anand R et al. Mic13 Is Essential for Formation of Crista Junctions in Mammalian Cells. PLoS One 11:e0160258 (2016). WB . PubMed: 27479602
- Chang HY et al. Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells. Cell Death Dis 5:e1540 (2014). IF ; Human . PubMed: 25429617
