Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-NDUFB6 antibody [21C11BC11] (ab110244)

Submit a review Q&A (1)References (30)
Western blot - Anti-NDUFB6 antibody [21C11BC11] (ab110244)
  • Flow Cytometry - Anti-NDUFB6 antibody [21C11BC11] (ab110244)

Key features and details

  • Mouse monoclonal [21C11BC11] to NDUFB6
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-NDUFB6 antibody [21C11BC11]
    See all NDUFB6 primary antibodies

  • Description

    Mouse monoclonal [21C11BC11] to NDUFB6

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa.
Notes
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa.

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

  • Sequence similarities

    Belongs to the complex I NDUFB6 subunit family.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • B17 antibody
    • CI antibody
    • CI B17 antibody
    • CI-B17 antibody
    • Complex I mitochondrial respiratory chain B17 subunit antibody
    • Complex I-B17 antibody
    • Complex IB17 antibody
    • MGC13675 antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 6 antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 antibody
    • NADH ubiquinone oxidoreductase B17 subunit antibody
    • NADH ubiquinone oxidoreductase beta subunit 6 antibody
    • NADH-ubiquinone oxidoreductase B17 subunit antibody
    • NDUB6_HUMAN antibody
    • NDUFB6 antibody
    see all

Images

  • Western blot - Anti-NDUFB6 antibody [21C11BC11] (ab110244)
    Western blot - Anti-NDUFB6 antibody [21C11BC11] (ab110244)
    All lanes : Anti-NDUFB6 antibody [21C11BC11] (ab110244) at 0.5 µg/ml

    Lane 1 : isolated mitochondria from Human heart at 5 µg
    Lane 2 : isolated mitochondria from cow heart at 1 µg
    Lane 3 : isolated mitochondria from rat heart at 10 µg
    Lane 4 : isolated mitochondria from mouse heart at 10 µg

    Predicted band size: 15 kDa

  • Flow Cytometry - Anti-NDUFB6 antibody [21C11BC11] (ab110244)
    Flow Cytometry - Anti-NDUFB6 antibody [21C11BC11] (ab110244)
    Overlay histogram showing HepG2 cells stained with ab110244 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110244, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References (30)

Publishing research using ab110244? Please let us know so that we can cite the reference in this datasheet.

ab110244 has been referenced in 30 publications.

  • Acoba MG  et al. The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism. Cell Rep 34:108869 (2021). PubMed: 33730581
  • D'Angelo L  et al. NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate. Cell Rep 35:109002 (2021). PubMed: 33882309
  • Kasahara T  et al. Cardiolipin is essential for early embryonic viability and mitochondrial integrity of neurons in mammals. FASEB J 34:1465-1480 (2020). PubMed: 31914590
  • Kalpage HA  et al. Brain-Specific Serine-47 Modification of Cytochrome c Regulates Cytochrome c Oxidase Activity Attenuating ROS Production and Cell Death: Implications for Ischemia/Reperfusion Injury and Akt Signaling. Cells 9:N/A (2020). PubMed: 32781572
  • Rouzier C  et al. NDUFS6 related Leigh syndrome: a case report and review of the literature. J Hum Genet N/A:N/A (2019). PubMed: 30948790
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Dear Tech support

Are antibodies tested for WB expected to work with BN-PAGE method and is it guaranteed?

More specificaly I'm asking about the following antibodies:

ab14713
ab55521
ab110244
ab14715
ab14745
ab14705

As always, Thank you for your kind suppot.
Reagrds,

Read More
Answer

Thank you for your enquiry.

I can confirm that the following antibodies are produced in our Mitosciences laboratories:


ab14713
ab110244
ab14715
ab14745
ab14705

These have been tested in blue native gel. These antibodies are also frequently referenced in the literature for Blue Native. An example is a paper attached showing these in BNPAGE.

The other antibody, ab55521 has not been tested in blue native gel. We do produce many products in house, but also source many externally. Therefore all our antibodieswill have been tested in different ways. Therefore, if an antibody has been tested in western blot, this does not mean it has also been tested in blue native gel. In this case, if you have the same question regarding any of our other antibodies, please do not hesitate to contact us again and we will be happy to confirm the information for you.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to let me know.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com