Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
![Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)](https://www.abcam.com/ps/products/110/ab110242/Images/ab110242-123418-anti-ndufb8-antibody-20e9dh10c12-western-blot.jpg "Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)")
Key features and details
- Mouse monoclonal [20E9DH10C12] to NDUFB8
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-NDUFB8 antibody [20E9DH10C12]
See all NDUFB8 primary antibodies -
Description
Mouse monoclonal [20E9DH10C12] to NDUFB8 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human
Predicted to work with: Pig
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Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart. Skeletal muscle tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
20E9DH10C12 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 22 kDa.
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| IHC-Fr |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 22 kDa. |
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IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Sequence similarities
Belongs to the complex I NDUFB8 subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4714 Human
- Entrez Gene: 67264 Mouse
- Entrez Gene: 293991 Rat
- Omim: 602140 Human
- SwissProt: O95169 Human
- SwissProt: Q9D6J5 Mouse
- Unigene: 523215 Human
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Alternative names
- ASHI antibody
- CI-ASHI antibody
- Complex I ASHI subunit antibody
see all
Images
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Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)All lanes : Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) at 0.5 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from cow heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Lane 4 : Isolated mitochondria from mouse heart at 10 µg
Predicted band size: 22 kDa
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs. -
![Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)](https://www.abcam.com/ps/products/110/ab110242/Images/ab110242-319473-anti-ndufb8-antibody-20e9dh10c12-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)Wedding, I.M. et al PLoS One. 2014 Jan 22;9(1):e86340. doi: 10.1371/journal.pone.0086340. eCollection 2014. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry in serial sections of the muscle of patient AIV-5
The patient is sufferening from Spastic paraplegia 7.
Immunohistochemistry for complex I (NDUFB8) (A), complex II (B), complex III (C) and COX/SDH histochemistry (D) in serial sections of the muscle of patient AIV-5. There are complex I, III and IV deficient fibres, but complex I deficiency is most pronounced. Arrows mark serial sections of the same muscle fibers stained for different complexes.
NDUFB8 (also refered to as complex I) was detected using ab110242 at 1/100 dilution).
(After Figure 3 of Wedding et al)
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![Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)](https://www.abcam.com/ps/products/110/ab110242/Images/ab110242-123417-anti-ndufb8-antibody-20e9dh10c12-immunohistochemistry.gif)
Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.
Datasheets and documents
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SDS download
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Datasheet download
References (189)
ab110242 has been referenced in 189 publications.
- Silva-Pinheiro P et al. A library of base editors for the precise ablation of all protein-coding genes in the mouse mitochondrial genome. Nat Biomed Eng 7:692-703 (2023). PubMed: 36470976
- Tian L et al. Microtubule Affinity-Regulating Kinase 4 Promotes Oxidative Stress and Mitochondrial Dysfunction by Activating NF-κB and Inhibiting AMPK Pathways in Porcine Placental Trophoblasts. Biomedicines 10:N/A (2022). PubMed: 35052845
- Marković A et al. Genetic Complementation of ATP Synthase Deficiency Due to Dysfunction of TMEM70 Assembly Factor in Rat. Biomedicines 10:N/A (2022). PubMed: 35203486
- Bury AG et al. A subcellular cookie cutter for spatial genomics in human tissue. Anal Bioanal Chem 414:5483-5492 (2022). PubMed: 35233697
- Antuña E et al. Removal of Environmental Nanoparticles Increases Protein Synthesis and Energy Production in Healthy Humans. Front Bioeng Biotechnol 10:800011 (2022). PubMed: 35237574