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Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)

Reviews (5)Q&A (3)References (165)
Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
  • Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
  • Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)

Key features and details

  • Mouse monoclonal [20E9DH10C12] to NDUFB8
  • Suitable for: WB, IHC-Fr
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-NDUFB8 antibody [20E9DH10C12]
    See all NDUFB8 primary antibodies

  • Description

    Mouse monoclonal [20E9DH10C12] to NDUFB8

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Pig

  • Immunogen

    Full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart. Skeletal muscle tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 22 kDa.
IHC-Fr
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 22 kDa.
IHC-Fr
Use at an assay dependent concentration.

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

  • Sequence similarities

    Belongs to the complex I NDUFB8 subunit family.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ASHI antibody
      • CI-ASHI antibody
      • Complex I ASHI subunit antibody
      • Complex I-ASHI antibody
      • mitochondrial antibody
      • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody
      • NADH-ubiquinone oxidoreductase ASHI subunit antibody
      • NDUB8_HUMAN antibody
      • NDUFB8 antibody
      see all

    Images

    • Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
      Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
      All lanes : Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) at 0.5 µg/ml

      Lane 1 : Isolated mitochondria from Human heart at 5 µg
      Lane 2 : Isolated mitochondria from cow heart at 1 µg
      Lane 3 : Isolated mitochondria from rat heart at 10 µg
      Lane 4 : Isolated mitochondria from mouse heart at 10 µg

      Predicted band size: 22 kDa



      Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
    • Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
      Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)Wedding, I.M. et al PLoS One. 2014 Jan 22;9(1):e86340. doi: 10.1371/journal.pone.0086340. eCollection 2014. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunohistochemistry in serial sections of the muscle of patient AIV-5

      The patient is sufferening from Spastic paraplegia 7.

      Immunohistochemistry for complex I (NDUFB8) (A), complex II (B), complex III (C) and COX/SDH histochemistry (D) in serial sections of the muscle of patient AIV-5. There are complex I, III and IV deficient fibres, but complex I deficiency is most pronounced. Arrows mark serial sections of the same muscle fibers stained for different complexes.

      NDUFB8 (also refered to as complex I) was detected using ab110242 at 1/100 dilution).

      (After Figure 3 of Wedding et al)

    • Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
      Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
      Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.

    References (165)

    Publishing research using ab110242? Please let us know so that we can cite the reference in this datasheet.

    ab110242 has been referenced in 165 publications.

    • Visuttijai K  et al. Progressive external ophthalmoplegia associated with novel MT-TN mutations. Acta Neurol Scand 143:103-108 (2021). PubMed: 32869280
    • He Q  et al. Mtu1 defects are correlated with reduced osteogenic differentiation. Cell Death Dis 12:61 (2021). PubMed: 33431792
    • Erskine D  et al. Lipids, lysosomes and mitochondria: insights into Lewy body formation from rare monogenic disorders. Acta Neuropathol 141:511-526 (2021). PubMed: 33515275
    • Oláhová M  et al. POLRMT mutations impair mitochondrial transcription causing neurological disease. Nat Commun 12:1135 (2021). PubMed: 33602924
    • Zhou Z  et al. A ketogenic diet impacts markers of mitochondrial mass in a tissue specific manner in aged mice. Aging (Albany NY) 13:7914-7930 (2021). PubMed: 33735837
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-NDUFB8 antibody [20E9DH10C12]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Primary Fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (12% TGX)
    Loading amount
    10 µg
    Treatment
    different treatments/cell lines
    Specification
    Human Primary Fibroblasts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 21 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NDUFB8 antibody [20E9DH10C12]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Skeletal muscle)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Sodium citrate, 0.05% Tween 20, pH 6.0
    Permeabilization
    Yes - 0.3% triton X100 in PBS
    Specification
    Skeletal muscle
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 06 2020

    Western blot abreview for Anti-NDUFB8 antibody [20E9DH10C12]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Human brain lysate)
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Loading amount
    10 µg
    Specification
    Human brain lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Sandrine Wauters

    Verified customer

    Submitted Apr 01 2016

    Western blot abreview for Anti-NDUFB8 antibody [20E9DH10C12]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (Mouse mitos from liver (A) and muscle (B))
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (3-12%)
    Loading amount
    10 µg
    Specification
    Mouse mitos from liver (A) and muscle (B)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 29 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NDUFB8 antibody [20E9DH10C12]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain- Cerebellum)
    Specification
    Brain- Cerebellum
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH8.0 EDTA
    Permeabilization
    No
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. Philippa Hepplewhite

    Verified customer

    Submitted Jun 28 2012

    Question

    I need the MSDS for the following products ordered today with order number:

    Anti-GRIM19 antibody [6E1BH7] (ab110240)
    Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)
    Anti-SDHB antibody [21A11AE7] (ab14714)
    Anti-SDHA antibody [2E3GC12FB2AE2] - Mitochondrial Marker (ab14715)
    Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
    Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)
    Anti-FH antibody [8F12BB5] (ab110286)
    Anti-Pyruvate Dehydrogenase E2 antibody [15D3G9C11 ] (ab110332)

    Read More
    Answer

    Thank you for contacting us.

    The MSDS for these products have been added to each product datasheet. They should appear on the website within the next few hours (time for the cache to be regenerated).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question

    Does kit work with Drosophila? How is the homology of the antibodies used in ab110413 with Drosophila?

    Read More
    Answer

    Thank you for your call earlier.

    I now had a look at the homology between the immunogens of the antibodies included in the MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (ab110413) and the respective proteins in Drosophila.

    Unfortunately, the homologies are only between 34% and 76%. This is not enough to expect a cross-reaction of the antibody cocktail.

    Therefore I cannot recommend ab110413 with drosophila samples.

    I am sorry I did not have a positive answer to your question on this occasion and wish you good luck with your research.

    Read More

    Question

    thank you for your fast reply. The antibody I`m using is Anti-Rt/Ms Total OXPHOS MS604/B0315. Thank you in advance!

    Read More
    Answer

    Thank you for this additional information. I can confirm that ab110413 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail) contains 5 antibodies. The datasheets and more information about these antibodies can be found when following these links to their respective datasheets: ab14705: Click here (or use the following: https://www.abcam.com/MTCO1-antibody-1D6E1A8-ab14705.html). ab14714: Click here (or use the following: https://www.abcam.com/SDHB-antibody-21A11AE7-ab14714.html). ab14745: Click here (or use the following: https://www.abcam.com/UQCRC2-antibody-13G12AF12BB11-ab14745.html). ab14748: Click here (or use the following: https://www.abcam.com/ATP5A-antibody-15H4C4-Mitochondrial-Marker-ab14748.html). ab110242: Click here (or use the following: https://www.abcam.com/NDUFB8-antibody-20E9DH10C12-ab110242.html). As mentioned in my previous email all these antibodies have purified full-length proteins as immunogens and the actual binding site of the monoclonal antibodies was not further mapped. The purified proteins were extracted from cow (and human in case of ab14705). I am sorry I cannot provide more exact immunogen information on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me with any further questions.

    Read More

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