Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)
![Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)](https://www.abcam.com/ps/products/110/ab110249/Images/ab110249-5-anti-NADH-dehydrogenase-ironsulfur-protein-2-antibody-7A12BE5AD5-immunocytochemistry-immunofluorescence-Hap1-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)")
Key features and details
- Mouse monoclonal [7A12BE5AD5] to NDUFS2
- Suitable for: WB, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-NDUFS2 antibody [7A12BE5AD5]
See all NDUFS2 primary antibodies -
Description
Mouse monoclonal [7A12BE5AD5] to NDUFS2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- WB: Isolated mitochondria from Human liver, Bovine heart, H9C2 cells (Rat cardiomyocyte) and MEF cells (Mouse embryo fibroblast). ICC/IF: HAP1, HDFn cultured cells (normal Human dermal fibroblasts, neonatal).
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
The purity of ab110249 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
7A12BE5AD5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.25 µg/ml. Predicted molecular weight: 53 kDa.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use a concentration of 0.25 µg/ml. Predicted molecular weight: 53 kDa. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Involvement in disease
Defects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
Sequence similarities
Belongs to the complex I 49 kDa subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 327697 Cow
- Entrez Gene: 4720 Human
- Entrez Gene: 226646 Mouse
- Entrez Gene: 289218 Rat
- Omim: 602985 Human
- SwissProt: O75306 Human
- SwissProt: Q91WD5 Mouse
- SwissProt: Q641Y2 Rat
see all -
Alternative names
- CI 49 antibody
- CI 49kD antibody
- CI-49kD antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)ab110249 staining NDUFS2 in wild-type Hap1 cells (top panel) and NDUFS2 knockout Hap1 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab110249 at 0.4μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
![Western blot - Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)](https://www.abcam.com/ps/products/110/ab110249/Images/ab110249-285150-anti-ndufs2-antibody-7a12be5ad5-western-blot.jpg)
Western blot - Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: NDUFS2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab110249 observed at 48 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab110249 was shown to specifically react with NDUFS2 in wild-type HAP1 cells whilst signal was lost in NDUFS2 knockout cells. Wild-type and NDUFS2 knockout samples were subjected to SDS-PAGE. ab110249 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 0.25 µg/ml and 1/20,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)](https://www.abcam.com/ps/products/110/ab110249/Images/ab110249-212977-anti-ndufs2-antibody-7a12be5ad5-flow-cytometry.jpg)
Flow Cytometry - Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)Overlay histogram showing HepG2 cells stained with ab110249 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110249, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Western blot - Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)](https://www.abcam.com/ps/products/110/ab110249/Images/ab110249-123435-anti-ndufs2-antibody-7a12be5ad5-western-blot.jpg)
Western blot - Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)All lanes : Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249) at 0.25 µg/ml
Lane 1 : Molecular Weight Markers
Lane 2 : Isolated mitochondria from Human liver
Lane 3 : Isolated mitochondria from Bovine heart
Lane 4 : Isolated mitochondria from H9C2 cells (Rat cardiomyocyte)
Lane 5 : Isolated mitochondria from MEF cells (Mouse embryo fibroblast)
Lane 6 : Isolated mitochondria from HepG2
Predicted band size: 53 kDa
Complex I-subunit NDUFS2 is detected in the Human samples with Human specific ab110249, while Complex I-subunit NDUFB8 is detected in all samples (Human, Mouse and Rat) with an anti-NDUFB8. -
![Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)](https://www.abcam.com/ps/products/110/ab110249/Images/ab110249-123434-anti-ndufs2-antibody-7a12be5ad5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)Mitochondrial localization of Complex I subunit NDUFS2 visualized by Immunocytochemistry in HDFn cultured cells (normal Human dermal fibroblasts, neonatal), using ab110249 at 5 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab110249 has been referenced in 8 publications.
- Sharanek A et al. OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation. Nat Commun 11:4116 (2020). PubMed: 32807793
- Formosa LE et al. Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly Complex Factors in the Biogenesis of Complex I. Cell Rep 31:107541 (2020). PubMed: 32320651
- Wakim J et al. CLUH couples mitochondrial distribution to the energetic and metabolic status. J Cell Sci 130:1940-1951 (2017). PubMed: 28424233
- Hernansanz-Agustín P et al. Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia. Redox Biol 12:1040-1051 (2017). WB . PubMed: 28511347
- Zurita Rendón O et al. A Mutation in the Flavin Adenine Dinucleotide-Dependent Oxidoreductase FOXRED1 Results in Cell-Type-Specific Assembly Defects in Oxidative Phosphorylation Complexes I and II. Mol Cell Biol 36:2132-40 (2016). PubMed: 27215383
- Coughlin CR et al. Mutations in the mitochondrial cysteinyl-tRNA synthase gene, CARS2, lead to a severe epileptic encephalopathy and complex movement disorder. J Med Genet 52:532-40 (2015). PubMed: 25787132
- Jacquemin G et al. Granzyme B-induced mitochondrial ROS are required for apoptosis. Cell Death Differ 22:862-74 (2015). PubMed: 25361078
- Zurita Rendón O et al. The arginine methyltransferase NDUFAF7 is essential for complex I assembly and early vertebrate embryogenesis. Hum Mol Genet 23:5159-70 (2014). PubMed: 24838397