Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)
![Western blot - Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)](https://www.abcam.com/ps/products/87/ab87399/Images/ab87399-102494-anti-ndufs4-antibody-2c7cd4ag3-western-blot.jpg "Western blot - Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)")
Key features and details
- Mouse monoclonal [2C7CD4AG3] to Ndufs4
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Ndufs4 antibody [2C7CD4AG3]
See all Ndufs4 primary antibodies -
Description
Mouse monoclonal [2C7CD4AG3] to Ndufs4 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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Positive control
- WB: isolated mitochondria from human, bovine, rat, and mouse heart tissue. Flow Cyt: HepG2 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES, Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
2C7CD4AG3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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| Notes |
|---|
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
Involvement in disease
Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
Sequence similarities
Belongs to the complex I NDUFS4 subunit family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 327680 Cow
- Entrez Gene: 4724 Human
- Entrez Gene: 17993 Mouse
- Entrez Gene: 499529 Rat
- Omim: 602694 Human
- SwissProt: Q02375 Cow
- SwissProt: O43181 Human
- SwissProt: Q9CXZ1 Mouse
see all -
Alternative names
- AQDQ antibody
- CI 18 antibody
- CI 18 kDa antibody
see all
Images
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Western blot - Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)All lanes : Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)
Lane 1 : Isolated mitochondria from human heart at 15 µg
Lane 2 : Isolated mitochondria from bovine heart at 6 µg
Lane 3 : Isolated mitochondria from rat heart at 30 µg
Lane 4 : Isolated mitochondria from mouse heart at 30 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa -
![Flow Cytometry - Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)](https://www.abcam.com/ps/products/87/ab87399/Images/ab87399-102493-anti-ndufs4-antibody-2c7cd4ag3-flow-cytometry.jpg)
Flow Cytometry - Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)Overlay histogram showing HepG2 cells stained with ab87399 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87399, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab87399 has been referenced in 15 publications.
- Esparza-Moltó PB et al. Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition. PLoS Biol 19:e3001252 (2021). PubMed: 33983919
- Hernansanz-Agustín P et al. Na+ controls hypoxic signalling by the mitochondrial respiratory chain. Nature 586:287-291 (2020). PubMed: 32728214
- Lee CF et al. Targeting NAD+ Metabolism as Interventions for Mitochondrial Disease. Sci Rep 9:3073 (2019). PubMed: 30816177
- Suo J et al. Met-enkephalin improves metabolic syndrome in high fat diet challenged mice through promotion of adipose tissue browning. Toxicol Appl Pharmacol 359:12-23 (2018). PubMed: 30222981
- Wang M et al. Mitochondrial complex I deficiency leads to the retardation of early embryonic development in Ndufs4 knockout mice. PeerJ 5:e3339 (2017). PubMed: 28533980