Recombinant Anti-NEK6 antibody [EPR5283] (ab133494)
![Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)](https://www.abcam.com/ps/products/133/ab133494/Images/ab133494-389438-anti-nek6-antibody-epr5283-western-blot-human-knockout-lysate.jpg "Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5283] to NEK6
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NEK6 antibody [EPR5283]
See all NEK6 primary antibodies -
Description
Rabbit monoclonal [EPR5283] to NEK6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, HeLa, 293T and HT1080 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5283 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
Target
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Function
Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation. -
Tissue specificity
Ubiquitous, with highest expression in heart and skeletal muscle. -
Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Clone ID: BC004174 Human
- Entrez Gene: 10783 Human
- Entrez Gene: 59126 Mouse
- Entrez Gene: 360161 Rat
- IMAGE Id: 2958695 Human
- Omim: 604884 Human
- SwissProt: Q9HC98 Human
- SwissProt: Q9ES70 Mouse
see all -
Alternative names
- Highly similar to cell cycle protein kinase CDC5/MSD2 antibody
- NEK6 antibody
- NEK6_HUMAN antibody
see all
Images
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Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)All lanes : Anti-NEK6 antibody [EPR5283] (ab133494) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NEK6 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 36 kDaLanes 1-3: Merged signal (red and green). Green - ab133494 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.
ab133494 Anti-NEK6 antibody [EPR5283] was shown to specifically react with NEK6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265910 (knockout cell lysate ab258072) was used. Wild-type and NEK6 knockout samples were subjected to SDS-PAGE. ab133494 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)](https://www.abcam.com/ps/products/133/ab133494/Images/ab133494-147154-anti-nek6-antibody-epr5283-western-blot.jpg)
Western blot - Anti-NEK6 antibody [EPR5283] (ab133494)All lanes : Anti-NEK6 antibody [EPR5283] (ab133494) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HT1080 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa -
![Anti-NEK6 antibody [EPR5283] (ab133494)](https://www.abcam.com/ps/products/133/ab133494/Images/ab133494-3-benefits-of-recombinant-antibodies.png)
Anti-NEK6 antibody [EPR5283] (ab133494)
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab133494 has been referenced in 7 publications.
- Chang H et al. Micro-ribonucleic acid-23a-3p prevents the onset of type 2 diabetes mellitus by suppressing the activation of nucleotide-binding oligomerization-like receptor family pyrin domain containing 3 inflammatory bodies-caused pyroptosis through negatively regulating NIMA-related kinase 7. J Diabetes Investig 12:334-345 (2021). PubMed: 32881354
- Gan W et al. LATS suppresses mTORC1 activity to directly coordinate Hippo and mTORC1 pathways in growth control. Nat Cell Biol 22:246-256 (2020). PubMed: 32015438
- Ting G et al. microRNA-219-5p targets NEK6 to inhibit hepatocellular carcinoma progression. Am J Transl Res 12:7528-7541 (2020). PubMed: 33312387
- Freixo F et al. NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization. Nat Commun 9:2330 (2018). PubMed: 29899413
- Choudhury AD et al. Castration Resistance in Prostate Cancer Is Mediated by the Kinase NEK6. Cancer Res 77:753-765 (2017). PubMed: 27899381
- Shi H et al. NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Nat Immunol 17:250-8 (2016). WB . PubMed: 26642356
- He Y et al. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature 530:354-7 (2016). PubMed: 26814970