Recombinant Anti-NEK7 antibody [EPR4900] (ab133514)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4900] to NEK7
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NEK7 antibody [EPR4900]
See all NEK7 primary antibodies -
Description
Rabbit monoclonal [EPR4900] to NEK7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, A549, C6, RAW264.7, PC12 and NIH/3T3 cell lysates. Wild-type HAP1 cell lysate. IP: NIH/3T3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4900 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/10000. Predicted molecular weight: 35 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/10000. Predicted molecular weight: 35 kDa. |
IP
1/10 - 1/100. |
Target
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Tissue specificity
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine. -
Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 140609 Human
- Entrez Gene: 59125 Mouse
- Entrez Gene: 360850 Rat
- Omim: 606848 Human
- SwissProt: Q8TDX7 Human
- SwissProt: Q9ES74 Mouse
- SwissProt: D3ZBE5 Rat
- Unigene: 24119 Human
see all -
Alternative names
- EC 2.7.11.1 antibody
- NEK7 antibody
- NEK7_HUMAN antibody
see all
Images
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ab133514 immunoprecipitating NEK7. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.
Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug
Lane 2: NIH/3T3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133514 in NIH/3T3 whole cell lysate -
All lanes : Anti-NEK7 antibody [EPR4900] (ab133514) at 1/10000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa -
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: NEK7 knockout HAP1 cell lysate (40 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133514 observed at 35 kDa. Red - loading control, ab18058, observed at 124 kDa.ab133514 was shown to specifically react with NEK7 when NEK7 knockout samples were used. Wild-type and NEK7 knockout samples were subjected to SDS-PAGE. Ab133514 and ab18058 (loading control to Vinculin) were diluted at 1/10000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-NEK7 antibody [EPR4900] (ab133514) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : A549 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : RAW264.7 cell lysate
Lane 5 : PC12 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (47)
ab133514 has been referenced in 47 publications.
- Liu C et al. Liquiritin Alleviates Depression-Like Behavior in CUMS Mice by Inhibiting Oxidative Stress and NLRP3 Inflammasome in Hippocampus. Evid Based Complement Alternat Med 2022:7558825 (2022). PubMed: 35069768
- Li J et al. A 4-Benzene-Indol Derivative Alleviates LPS-Induced Acute Lung Injury Through Inhibiting the NLRP3 Inflammasome. Front Immunol 13:812164 (2022). PubMed: 35222388
- Lin H et al. An RRx-001 Analogue With Potent Anti-NLRP3 Inflammasome Activity but Without High-Energy Nitro Functional Groups. Front Pharmacol 13:822833 (2022). PubMed: 35250572
- Li C et al. Inhibition of the NLRP3 Inflammasome Activation by Manoalide Ameliorates Experimental Autoimmune Encephalomyelitis Pathogenesis. Front Cell Dev Biol 10:822236 (2022). PubMed: 35252186
- Liang L et al. Oridonin relieves depressive-like behaviors by inhibiting neuroinflammation and autophagy impairment in rats subjected to chronic unpredictable mild stress. Phytother Res 36:3335-3351 (2022). PubMed: 35686337