Anti-NET1 antibody (ab113202)
Key features and details
- Rabbit polyclonal to NET1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NET1 antibody
See all NET1 primary antibodies -
Description
Rabbit polyclonal to NET1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 546-596 of Human NET1 (NP_001040625).
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Positive control
- WB: Jurkat whole cell lysate and HEK293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 62 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| Notes |
|---|
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WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 62 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10276 Human
- SwissProt: Q7Z628 Human
- Unigene: 25155 Human
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Alternative names
- ARHG8_HUMAN antibody
- ARHGEF8 antibody
- GLYT2 antibody
see all
Images
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Immunoprecipitation - Anti-NET1 antibody (ab113202)
Immunoprecipitation of NET1 (6 µg of antibody per mg of HEK-293T cell lysate; 20% of the IP loaded). Anti-Net 1 antibody (ab113202) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 seconds.
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Western blot - Anti-NET1 antibody (ab113202)All lanes : Anti-NET1 antibody (ab113202) at 0.1 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 62 kDa
Exposure time: 30 secondsDetected by chemiluminescence.
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Immunoprecipitation - Anti-NET1 antibody (ab113202)Immunoprecipitation of NET1 (6 µg of antibody per mg of Jurkat cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-Net1 antibody that recognizes an upstream epitope; Lane 2- Immunoprecipitation with ab113202; Lane 3- Immunoprecipitation with IgG control. Anti-Net 1 antibody (ab113202) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 3 min.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab113202 has been referenced in 2 publications.
- Zhao F et al. Baicalin suppresses lung cancer growth phenotypes via miR-340-5p/NET1 axis. Bioengineered 12:1699-1707 (2021). PubMed: 33955315
- Liang X et al. VTIQ evaluates antitumor effects of NET-1 siRNA by UTMD in HCC xenograft models. Oncol Lett 16:2893-2902 (2018). PubMed: 30127877