Recombinant Anti-NeuN antibody [EPR12763] - Rat IgG2a (ab279297)
![Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Rat IgG2a (ab279297)](https://www.abcam.com/ps/products/279/ab279297/Images/ab279297-451032-ab279309520201223immunocytochemistryab279297epr12763rtigg2aneunshsy5y1ugpfa.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Rat IgG2a (ab279297)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [EPR12763] to NeuN - Rat IgG2a
- Suitable for: WB, ICC/IF, Flow Cyt (Intra), IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NeuN antibody [EPR12763] - Rat IgG2a
See all NeuN primary antibodies -
Description
Rat monoclonal [EPR12763] to NeuN - Rat IgG2a -
Host species
Rat -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra), IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, mouse and rat brain tissue lysate. Flow Cyt (intra): Rat primary neural/glia cells. IHC: Human cerebral cortex. ICC/IF: SHSY5Y cells.
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General notes
This rat antibody has been engineered from a RabMAb parent antibody (ab177487). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12763 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab279297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000.
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| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
1/1000.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
1/1000. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
RNA-binding protein that regulates alternative splicing events. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 146713 Human
- Entrez Gene: 52897 Mouse
- Entrez Gene: 287847 Rat
- SwissProt: A6NFN3 Human
- SwissProt: Q8BIF2 Mouse
- Unigene: 135229 Human
- Unigene: 341103 Mouse
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Alternative names
- FLJ56884 antibody
- FLJ58356 antibody
- Fox-1 homolog C antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Rat IgG2a (ab279297)
Immunofluorescence staining of NeuN using ab279297 in human SHSY5Y cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab279297 at 1.0 μg/ml. Cells were then incubated with ab150165, Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and nuclear DNA was labelled with DAPI (shown in blue). The secondary only control (bottom row) was not incubated with ab279297 but otherwise processed the same. Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] (ab279297)](https://www.abcam.com/ps/products/279/ab279297/Images/ab279297-5-antineunepr12763antibodyimmunohistochemistrycerebralcortexhumanyr061604psrtigg2a.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] (ab279297)IHC image of NeuN staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab279297, 1ug/ml, for 15 mins at room temperature. A rabbit anti-rat IgG2a, ab102248, was added for 8 mins at room temperature and detected using an HRP conjugated goat anti-rabbit compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Rat IgG2a (ab279297)](https://www.abcam.com/ps/products/279/ab279297/Images/ab279297-451031-ab279309620201223immunocytochemistryab279297epr12763rtigg2aneunshsy5y1ugmeoh.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Rat IgG2a (ab279297)Immunofluorescence staining of NeuN using ab279297 in human SHSY5Y cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab279297 at 1.0 μg/ml. Cells were then incubated with ab150165, Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and nuclear DNA was labelled with DAPI (shown in blue). The secondary only control (bottom row) was not incubated with ab279297 but otherwise processed the same. Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-NeuN antibody (ab279297)All lanes : Anti-NeuN antibody [EPR12763] - Rat IgG2a (ab279297) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilutionExposure time: Lane 1: 48 seconds; Lane 2, 3: 4.5 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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Flow Cytometry (Intracellular) - Anti-NeuN antibody (ab279297)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized rat primary neural/glia cells labelling NeuN with ab279297 at 1/1000 dilution (0.1µg)/ Right compared with a Mouse monoclonal IgG isotype control/ Left.
Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 647, ab150163) at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab279297 has been referenced in 3 publications.
- Fu Q et al. P-Rex2 mediation of synaptic plasticity contributes to bone cancer pain. Mol Pain 18:17448069221076460 (2022). PubMed: 35083941
- Numano R et al. Nanoscale-tipped wire array injections transfer DNA directly into brain cells ex vivo and in vivo. FEBS Open Bio 12:835-851 (2022). PubMed: 35293154
- Fu J et al. Spinal Nrf2 translocation may inhibit neuronal NF-?B activation and alleviate allodynia in a rat model of bone cancer pain. J Neurochem 158:1110-1130 (2021). PubMed: 34254317